Nematology - Concepts, Diagnosis and Control 2017
DOI: 10.5772/intechopen.69075
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Molecular Diagnostic Tools for Nematodes

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Cited by 10 publications
(3 citation statements)
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“…An interesting finding from the comparison of these two diagnostic methods is that an additional 41.2% of microscopy-negative samples were found T. trichiura- positive when assessed by qPCR. We hypothesise that lower DNA loads found in Kato-Katz-negative samples reflect higher detection rates by qPCR due to a higher sensitivity rather than lower specificity of the qPCR assays as remaining DNA of already dead worms or eggs can still act as a template DNA during qPCR [ 43 ].…”
Section: Discussionmentioning
confidence: 99%
“…An interesting finding from the comparison of these two diagnostic methods is that an additional 41.2% of microscopy-negative samples were found T. trichiura- positive when assessed by qPCR. We hypothesise that lower DNA loads found in Kato-Katz-negative samples reflect higher detection rates by qPCR due to a higher sensitivity rather than lower specificity of the qPCR assays as remaining DNA of already dead worms or eggs can still act as a template DNA during qPCR [ 43 ].…”
Section: Discussionmentioning
confidence: 99%
“…Even though molecular diagnosis of nematodes may be objective and straightforward [ 46 ], a proper nematode identification should include morphological analysis of specimens [ 14 ]. Molecular data complement morphological observations of worms, which may lead to the identification of cryptic diversity sensu lato or sensu stricto, and the reclassification of certain taxa or the description of new species [ 18 ].…”
Section: Discussionmentioning
confidence: 99%
“…As mentioned above, parasitology requires constant updating since taxonomic classification of organisms may change with new molecular, ecological and morphological findings [ 25 ]. In addition, diagnostics such as DNA-based techniques may be developed with improved sensitivity and specificity as well as simplified accessibility [ 26 , 27 ]. However, interestingly, the popularity of pets may change, like for reptiles and amphibians; thus, updates on their associated parasites and potential role as reservoirs should be studied.…”
Section: Lectures and Practical Activities: From Theory To Practicementioning
confidence: 99%