Molecular diversity, haplotype distribution and genetic variation flow of Bipolaris sorokiniana fungus causing spot blotch disease in different wheat-growing zones

Kashyap, Prem Lal; Kumar, Sudheer; Sharma, Anju; Kumar, Ravi; Mahapatra, Sunita; Kaul, Noyonika; Khanna, Annie; Jasrotia, Poonam; Singh, Gyanendra Pratap

doi:10.1007/s13353-022-00716-w

Cited by 8 publications

(1 citation statement)

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“…GenAlEx version 6.503 [ 32 ] was used to estimate the basic statistics, such as major allele frequency, the number of alleles per locus, heterozygosity, polymorphic information content (PIC), and gene diversity. The PIC value for each SSR marker was estimated using the formula as described by Kashyap et al (2022) [ 33 ]. Additionally, Shannon’s information index, observed heterozygosity, expected heterozygosity, observed heterozygosity, number of private alleles per locus, banding pattern across the population, and number of effective alleles per locus were estimated for each population.…”

Section: Methodsmentioning

confidence: 99%

Development of Diagnostic Markers and Applied for Genetic Diversity Study and Population Structure of Bipolaris sorokiniana Associated with Leaf Blight Complex of Wheat

Kashyap

Manzar

Maurya

et al. 2023

JoF

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Bipolaris sorokiniana, a key pathogenic fungus in the wheat leaf blight complex, was the subject of research that resulted in the development of fifty-five polymorphic microsatellite markers. These markers were then used to examine genetic diversity and population structure in Indian geographical regions. The simple sequence repeat (SSR) like trinucleotides, dinucleotides, and tetranucleotides accounted for 43.37% (1256), 23.86% (691), and 16.54% (479) of the 2896 microsatellite repeats, respectively. There were 109 alleles produced by these loci overall, averaging 2.36 alleles per microsatellite marker. The average polymorphism information content value was 0.3451, with values ranging from 0.1319 to 0.5932. The loci’s Shannon diversity varied from 0.2712 to 1.2415. These 36 isolates were divided into two main groups using population structure analysis and unweighted neighbour joining. The groupings were not based on where the isolates came from geographically. Only 7% of the overall variation was found to be between populations, according to an analysis of molecular variance. The high amount of gene flow estimate (NM = 3.261 per generation) among populations demonstrated low genetic differentiation in the entire populations (FST = 0.071). The findings indicate that genetic diversity is often minimal. In order to examine the genetic diversity and population structure of the B. sorokiniana populations, the recently produced microsatellite markers will be helpful. This study’s findings may serve as a foundation for developing improved management plans for the leaf blight complex and spot blotch of wheat diseases in India.

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Section: Methodsmentioning

confidence: 99%