Molecular epidemiology of vancomycin-resistant Enterococcus faecium in Argentina

Corso, Alejandra; Gagetti, Paula; Rodríguez, Marisa; Melano, Roberto G.; Ceriana, Paola; Faccone, Diego; Galas, Marcelo

doi:10.1016/j.ijid.2006.02.003

Cited by 37 publications

(33 citation statements)

References 39 publications

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“…22% of enterococcal infections were caused by multi-resistant E. faecium, similarly to what was reported in the US [23]. In Argentina, Corso et al [24], through a nation-wide antimicrobial resistance survey, proved that most of vancomycin resistant enterococci carried vanA gene (98%). In addition, these strains, expressed high-level resistance to gentamicin (77.2%) and streptomycin (95.8%).…”

Section: Introductionsupporting

confidence: 52%

“…In different hospitals predominance of one epidemic clone carrying vanA has been observed, with an increased incidence of vancomycin-resistant E. faecium clonal spread [24]. In 2014, according to the Antimicrobial Resistance Surveillance Network WHONET-Argentina (89 Microbiology Laboratories), clinical E. faecium shows a high rate (61%) of vancomycin resistance (www.antimicrobianos.com.ar).…”

Section: Discussionmentioning

confidence: 99%

“…Chromosomal DNA was prepared in agarose plugs, digested with the restriction enzyme SmaI. DNA fragments were separated in a CHEF-DRIII apparatus (Bio-Rad Laboratories, Richmond, CA, USA), following the protocol of Corso et al [24]. SmaI was chosen upon the base composition (%G+C content) of the DNA of the microorganisms in study.…”

Section: Pfge Typingmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

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Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n:13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n:7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.

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Section: Introductionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

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“…This species has been recognized as the third leading cause of healthcare-associated bloodstream infections in the USA, generating an enormous cost burden for the healthcare system [3]. Enterococci have gained resistance to almost the entire antimicrobial spectrum, including vancomycin, undoubtedly the most potential antimicrobial agent as a treatment for enterococcal infections [4]. In 1986, vancomycin-resistant Enterococcus (VRE) was initially was reported from clinical isolates in the UK and France.…”

Section: Introductionmentioning

confidence: 99%

“…In 1986, vancomycin-resistant Enterococcus (VRE) was initially was reported from clinical isolates in the UK and France. According to the data, a year later VRE was documented in US hospitals and emerged as one of the crucial hospital-acquired infectious agents [4]. In 2004, VRE strains were initially reported in Iran; and presently pertain to be a highly relevant nosocomial pathogen [5,6].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of vancomycin-resistant Enterococcus in Iran: a systematic review and meta-analysis

Emaneini

Hosseinkhani

Jabalameli

et al. 2016

Eur J Clin Microbiol Infect Dis

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Vancomycin-resistant Enterococcus (VRE) is considered to be a major nosocomial pathogen that results in serious morbidity and mortality worldwide. Limited information is available concerning the prevalence of VRE infections in Iran. We carried out a systematic search by using different electronic databases including: Medline (via PubMed), Embase, Web of Science, and the Iranian Database. Meta-analysis was performed using comprehensive meta-analysis software. The meta-analyses revealed that the prevalence of VRE infections was 9.4 % (95 % confidence interval [95 % CI] 7.3-12) among culture-positive cases for Enterococcus species. The prevalence of VRE in Iran is compared with the results of developed countries. The prevalence of VRE in Germany, the United Kingdom (UK), and Italy was 11.2 %, 8.5-12.5 %, and 9 % respectively. Additionally, the frequency of vancomycin resistance among E. faecalis isolates was higher than for E. faecium. The results of this study indicate that a comprehensive infection control strategy based on hand hygiene, educating the hospital staff members, providing clinical guidance and principles for the appropriate use of antibiotics, sanitizing the hospitals, contact precautions, and active surveillance systems on the basis of international criteria is urgently needed.

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Problematic Groups of Multidrug-Resistant Bacteria and Their Resistance Mechanisms

Kohler

Vaishampayan

Grohmann

2019

Antibacterial Drug Discovery to Combat MDR

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Molecular epidemiology of vancomycin-resistant Enterococcus faecium in Argentina

Abstract: The emergence of vancomycin-resistant Enterococcus faecium in Argentina seems to be related to the intra- and inter-hospital dissemination of an epidemic clone carrying the vanA element.

Cited by 37 publications

References 39 publications

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Prevalence of vancomycin-resistant Enterococcus in Iran: a systematic review and meta-analysis

Problematic Groups of Multidrug-Resistant Bacteria and Their Resistance Mechanisms

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