Molecular evidence for hybrid origin of Aster chusanensis, an endemic species of Ulleungdo, Korea

Shin, Hyunchur; Oh, Sang‐Hun; Lim, Yongseok; Hyun, Chang-Woo; Cho, Seong-Hyun; Kim, Young Dong

doi:10.1007/s12374-014-0135-9

Cited by 19 publications

(14 citation statements)

References 55 publications

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“…The island is very small with a total area of 73 km 2 , its highest peak rises up to 984 m, and it contains approximately 500 native vascular plant species, about 30-40 of which are endemic to the island (Hong et al 2002;Jung et al 2013;Sun et al 2014;Yang et al 2015). Although it was recently confirmed that some of the endemic species originated via hybridization (Gil and Kim 2016;Shin et al 2014), the predominant mode of generating the diversity of endemic plant lineages on Ulleung Island was considered anagenetic speciation. Several studies have confirmed the origin of endemic vascular plant species via anagenesis and have addressed the genetic consequences of anagenetic speciation (e.g., Pfosser et al 2002Pfosser et al , 2006Pfosser et al , 2011Takayama et al 2012Takayama et al , 2013.…”

Section: Introductionmentioning

confidence: 99%

Origin of the critically endangered endemic species Scrophularia takesimensis (Scrophulariaceae) on Ulleung Island, Korea: implications for conservation

et al. 2020

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Scrophularia takesimensis is a critically endangered endemic species of Ulleung Island, Korea. A previous molecular phylogenetic study based on nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequences with very limited sampling suggested that it is most closely related to the clade comprising S. alata and S. grayanoides. To determine the origin of S. takesimensis, we sampled a total of 171 accessions including S. takesimensis (9 populations and 63 individuals) and two closely related species, S. alata (11 populations and 68 individuals) and S. grayanoides (5 populations and 40 individuals) from eastern Asia and sequenced ITS and two chloroplast DNA (cpDNA) non-coding regions. Previously sequenced representative species of Scrophularia (109 taxa for ITS and 80 taxa for cpDNA) were combined with our data set and analyzed. While the global scale ITS phylogenetic tree suggests monophyly for each of the three eastern Asian species, S. takesimensis appears to be more closely related (albeit weakly) to a clade containing eastern North American/Caribbean species than to either S. alata or S. grayanoides. By contrast, the global scale cpDNA phylogenetic tree demonstrates that the eastern North America/Caribbean clade is sister to a clade comprising the three eastern Asian species. In addition, the monophyletic S. takesimensis is deeply embedded within paraphyletic S. alata, sharing its most recent common ancestor with populations from Japan/Sakhalin. Two divergent, geographically structured cp haplotype groups within S. takesimensis suggest at least two independent introductions from different source areas. A new and accurate chromosome number of S. takesimensis (2n = 94) is reported and some conservation strategies are discussed.

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Section: Introductionmentioning

confidence: 99%

Origin of the critically endangered endemic species Scrophularia takesimensis (Scrophulariaceae) on Ulleung Island, Korea: implications for conservation

et al. 2020

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“…PCR-mediated recombination results from either polymerase template switching during PCR or annealing of prematurely terminated products to non-homologous templates [ 43 , 44 ]. This phenomenon has been well characterized from other hybrid plant species including Potamogeton intortusifolius [ 45 ], Malus toringoides [ 46 ] and Aster chusanensis [ 47 ]. In this study, the detection of chimeric haplotypes (H3) from an artificial DNA template (1:1 DNA mixture of C .…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)

et al. 2021

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Natural hybridization has been considered a source of taxonomic complexity in Cryptocoryne. A combined study of DNA sequencing data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA and the trnK-matK region of chloroplast DNA was used to identify the parents of Cryptocoryne putative hybrids from Peninsular Malaysia. Based on the intermediate morphology and sympatric distribution area, the plants were tentatively identified as the hybrid Cryptocoryne ×purpurea nothovar. purpurea. The plants were pollen sterile and had long been considered as hybrids, supposedly between two related and co-existing species, C. cordata var. cordata and C. griffithii. The status of C. ×purpurea nothovar. purpurea was independently confirmed by the presence of an additive ITS sequence pattern from these two parental species in hybrid individuals. An analysis of the chloroplast trnK-matK sequences showed that the hybridization is bidirectional with the putative hybrids sharing identical sequences from C. cordata var. cordata and C. griffithii, indicating that both putative parental species had been the maternal parent in different accessions.

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“…Recent studies, particularly ones based on DNA analysis, revealed that the features of indica and japonica have been found in the wild relatives of Oryza sativa (Qiu et al 2015). Such genetic differentiation of weedy rice populations is likely to be associated with the introgression from their two locally corresponding subspecies of rice, indica and japonica (Craig et al 2014;Shin et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, this analysis of the origin of weedy rice by means of sequences of nrDNA ITS and chloroplast DNA is for the first time. Sequences of nrDNA ITS and cpDNA can complement each other and the combination of them can improve the ability to discriminate at the species or population level due to their different pattern of inheritance (Shin et al 2014;Carlos et al 2015). Here, the multi-locus DNA sequences provided a similar grouping pattern for the Chinese weedy rice accessions.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is known that phylogenetic hypotheses based on a single gene or character may not represent true organism relationships and single DNA sequence probably restricted reality and reliability in the study. The combined use of nuclear and chloroplast DNA (cpDNA) data would help researchers to comprehensively understand the origin and evolutionary history of the weedy rice (Shin et al 2014;Carlos et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Molecular phylogeny of the Chinese weedy rice based on nrDNA ITS and two cpDNA sequences

Liu¹,

Shiqi²,

Zhai³

et al. 2016

Ind. Jrnl. Gen. Plnt. Bree.

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Direct sequencing data from the nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) region and two chloroplast DNA (cpDNA) fragments (psbA-trnH and petG-trnP) of Chinese weedy rice (Oryza stavia L. f. sp. spontanea) were analyzed separately and simultaneously in the MEGA 6.0 software. The nrDNA, cpDNA and cpDNA+nrDNA phylogenetic trees showed that the weedy rice collected from Northern China, Oryza sativa Japonica group and Oryza rufipogon, were on the one phylogenic node whereas weedy rice were collected from Southern China and Oryza sativa Indica group were on the other phylogenic node. The results revealed that there were geographical and indica-japonica differentiations in Chinese weedy rice which were corresponding to their geographical distribution and cultivated rice (Oryza sativa) indica-japonica differentiation. These results demonstrated that Chinese weedy rice from different regions have diverse origins and they were the relative origin group of the cultivated rice as there is no wild form available in Northern China. Additionally, clone sequence variation of the nrDNA ITS regions in intraindividual weedy rice provides evidence origin of other Oryza taxa through the process of hybridization or introgression. Data from this research further confirmed that the Chinese weedy rice was evolved from local rice varieties (as the female parent) which naturally hybridized with other oryza materials.

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Molecular evidence for hybrid origin of Aster chusanensis, an endemic species of Ulleungdo, Korea

Cited by 19 publications

References 55 publications

Origin of the critically endangered endemic species Scrophularia takesimensis (Scrophulariaceae) on Ulleung Island, Korea: implications for conservation

Origin of the critically endangered endemic species Scrophularia takesimensis (Scrophulariaceae) on Ulleung Island, Korea: implications for conservation

Molecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)

Molecular phylogeny of the Chinese weedy rice based on nrDNA ITS and two cpDNA sequences

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