Molecular evidence for the occurrence of tomato leaf curl New Delhi virus on chayote (Sechium edule) in southern India

Nagendran, K.; Mohankumar, S.; Faisal, P. Mohammed; Basavaraj, B.; Karthikeyan, G.

doi:10.1007/s13337-017-0403-7

Cited by 17 publications

(8 citation statements)

References 13 publications

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“…The ToLCDNV can cause symptoms of leaf yellow spots on cucumber, and symptoms of leaf curling and yellow spots on pumpkin, leaf curling and yellowing on bottle gourd, and leaf curling and blistering on wax gourd. Those symptoms are similar to what was previously described [30,[38][39][40][41]. ToLCNDV-infected oriental melon and tobacco plants developed symptoms of leaf curling and blistering, which are also similar to those previously described [42,43]; however, both plants infected by pseudorecombinant ToLCNDV-A[MY-BG120-17] exhibited severe symptoms of leaf curling, blistering, necrosis and yellow spots.…”

Section: Discussionsupporting

confidence: 87%

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Chen

Lai

Lin

et al. 2021

Plants

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Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of Squash leaf curl China virus (SLCCNV) and Tomato leaf curl New Delhi virus (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.

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Section: Discussionsupporting

confidence: 87%

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Chen

Lai

Lin

et al. 2021

Plants

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“…crops in these regions. ToLCNDV was found to cause a yield loss of more than 60%, with a maximum disease incidence of 100% for chayote (Sechium edule) in India (Nagendran et al, 2017). Up to 22% of zucchini crops cultivated in the greenhouses at Almeria were affected by ToLCNDV (Crespo et al, 2020), and melon yield losses were approximately 20% in open fields in central Spain (Sáez et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…), bottle gourd (Lagenaria siceraria), Luffa spp., and Sechium edule. (Chang et al, 2010;Ito et al, 2008;Nagendran et al, 2017;Sohrab et al, 2003;Phaneendra et al, 2012). Since 2012, ToLCNDV has spread westward, with reports from the Middle East, Spain, Portugal, Italy, Greece, Morocco, Tunisia, and Algeria in the Mediterranean Basin (EPPO, 2020;Juárez et al, 2014;Kheireddine et al, 2019;Mnari-Hattab et al, 2015;Orfanidou et al, 2019;Panno et al, 2016;Ruiz et al, 2015;Sifres et al, 2018;Yazdani-Khameneh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Southeast Asian Isolate of the Tomato Leaf Curl New Delhi Virus Shows Higher Pathogenicity Against Tomato and Cucurbit Crops Compared to that of the Mediterranean Isolate

et al. 2021

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The tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (genus Begomovirus, family Geminiviridae) that was originally isolated in India, but has become a serious threat to various horticultural crops in the Indian subcontinent, Southeast Asia, East Asia, Middle East, and western Mediterranean Basin. In Southeast Asia, East Asia, and the western Mediterranean Basin, ToLCNDV has caused huge economic damage to cucurbit crops (cucumber, melon, squash, and zucchini) for which commercial ToLCNDV-resistant cultivars are currently unavailable. In this study, full-length ToLCNDV viral sequences were isolated from cucumber, melon, and zucchini plants exhibiting yellow leaf curl disease in Almeria, Spain. Phylogenetic analysis showed that the isolated viruses consisted of the ToLCNDV-ES strain and strains that were genetically distant from ToLCNDV- in Southeast Asia, which were previously isolated in the Aceh province of Indonesia. Infectious clones of ToLCNDV-[ES-Alm-Cuc-16] from Spain and of ToLCNDV-[BACu-20] from Indonesia were prepared, and ToLCNDV isolates were agroinoculated to Nicotiana benthamiana, tomato, and cucurbit crops (cucumber, melon, and zucchini). Infection rates were nearly 100% for all inoculated plant species, and disease symptoms were consistently more severe in plants inoculated with ToLCNDV-[BACu-20] compared to plants inoculated with ToLCNDV-[ES-Alm-Cuc-16]. For tomatoes inoculated with ToLCNDV-[ES-Alm-Cuc-16], infection occurred without symptoms in most plants, and only a few plants showed slight vein-yellowing symptoms. In contrast, ToLCNDV-[BACu-20] infection induced typical begomoviral disease symptoms of yellowing and curling of leaves in the same tomato cultivar.The present study highlights the importance of screening genetic resources for ToLCNDV resistance by using ToLCNDV isolates from the intended production area; furthermore, the agroinoculation method used in this study will facilitate breeding resistance against both the Southeast Asian and Mediterranean ToLCNDV isolates.

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“…These results showed that despite of co-circulation of different satellites in the same region, this virus has not captured any of them. Earlier cucurbit hosts were infected with ToLCNDV (Nagendran et al 2017;Fortes et al 2016;Nagendran et al 2016) but now it is becoming clear that a new bipartite begomovirus ToL-CPMV is becoming a major viral threat to cucurbits. The intercropping of cucurbits and other crops such as chilies, cotton and especially tomato (a natural host for ToLCPMV) is a common practice in the farmer's fields, so they may become the infection source for the next tomato-growing season.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

et al. 2019

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Leaf samples of Cucumis Sativus L. (C. sativus) (Family; Cucurbitaceae) showing vein thickening, mild leaf curling and leaf enations were collected from the farmer's field. Amplification of the full-length viral molecules was performed through rolling circle amplification (RCA). Cloning of the full-length viral molecules was done through standard cloning procedure followed by sequencing. Sequence similarity analysis and phylogenetic studies showed that the virus associated with leaf curling and enations in C. sativus was a bipartite begomovirus, where DNA-A and DNA-B showed highest nucleotide sequence homology of 98% and 97% to tomato leaf curl Palampur virus (ToLCPMV) from India. Attempts to isolate betasatellites and alphasatellites through PCR using RCA product as template, did not result in any amplification. A maximum likelihood phylogenetic tree grouped DNA-A and B components with other isolates from India. SDT was used to find the pairwise identity scores of different sequences of ToLCPMV present in the database. Phylogenetic analysis showed that sequences of ToLCPMV DNA-A and B components in this study share high degree of homology with existing viruses and are isolates of ToLCPMV-India. Infectious molecules of both components (Accessions, MG252783 and MG252784, respectively) were constructed for infectivity analysis to fulfill the Koch's postulate. Infectivity analysis revealed that ToLCPMV DNA-A is infectious to model host plant Nicotiana benthamiana and viral accumulation was confirmed through Southern blot analysis. Accumulation of DNA-B was confirmed through PCR. Infectivity analysis was also conducted using the original host, C. sativus, but plants were unable to survive the agroinoculation. To our knowledge this is the first report of ToLCPMV associated with C. sativus L. in Pakistan.

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Molecular evidence for the occurrence of tomato leaf curl New Delhi virus on chayote (Sechium edule) in southern India

Cited by 17 publications

References 13 publications

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Southeast Asian Isolate of the Tomato Leaf Curl New Delhi Virus Shows Higher Pathogenicity Against Tomato and Cucurbit Crops Compared to that of the Mediterranean Isolate

Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

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