Molecular evidence that the whitefly-transmitted sweetpotato mild mottle virus belongs to a distinct genus of thePotyviridae

Colinet, Dominique; Kummert, J.; Lepoivre, Philippe

doi:10.1007/bf01718593

Cited by 37 publications

(26 citation statements)

References 32 publications

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“…Serological and PCR procedures have been developed to detect viruses from sweetpotato vines (Gibbs and Padovan, 1993;Colinet et al, 1996;Alicai et al, 1999;Kreuze et al, 2000). However, these procedures were designed to test for a single virus per reaction and this increases the amount of reagents, time and labour required to test each sample.…”

Section: Introductionmentioning

confidence: 99%

Detection and elimination of sweetpotato viruses

Rukarwa

Mashingaidze²,

Kyamanywa³

et al. 2011

African Crop Science Journal

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In sub-Saharan Africa, sweetpotato (Impomoea batatas L.) production is greatly constrained by sweetpotato virus disease (SPVD) complex. This study was conducted to assess the incidence of viruses in healthy-looking sweetpotato in Uganda and to optimise modern technologies for virus diagnosis. A collection of healthy-looking sweetpotato vines from central Uganda were serologically assayed for sweetpotato viruses and the positive samples were confirmed by RT-PCR. A multiplex RT-PCR assay was optimised for simultaneous detection of Sweet potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV). The use of in vitro thermotherapy was also investigated as a means of eliminating sweetpotato viruses. Four viruses namely SPCSV, SPFMV, SPMMV and SPCFV were detected mostly as single infections in the healthy looking plants. SPCSV (70. 6%) recorded highest incidence followed by co-infection of SPFMV and SPCSV (8.3%). Based on shoot survival and effectiveness of virus elimination, the best results were obtained by exposing plantlets to daily temperature regime of 32 RÉSUMÉEn Afrique sub saharienne, la production de la patate douce (Impomoea batatas L.) est grandement affectée par un complexe de maladies de virus (SPVD). Cette étude était conduite pour évaluer l'incidence maladie des virus sur des boutures apparemment saines de la patate douce en Ouganda et optimiser les technologies pour diagnostic de virus. Des boutures apparemment saines de patate douce collectées au centre de l'Ouganda étaient sérologiquement testées et les échantillons infectés étaient confirmés par RT-PCR. Un essai multiplexe RT-PCR était optimisé pour la detection simultanée du virus du rabougrissement chlorotique de la patate douce (SPCSV), le virus de la marbrure duveteuse de la patate (SPSMV) et le virus de marbrure modérée de la patate douce (SPMMV). L'usage de la thermothérapie in vitro était aussi testé comme moyen d'élimination des virus de la patate. Quatre virus dont SPCSV, SPFMV, SPMMV et SPCFV étaient detectés surtout comme seules infections des plantes apparemment saines. Le SPCSV (70. 6%) avait présenté une incidence élevée, suivi de SPFMV et SPCSV dont le niveau d'infection était le même (8.3%). Basé sur la survie des pousses et l'efficacité de l'élimination de virus, les meilleurs résultats étaient obtenus en exposant les plantules à un regime de température de 32 o C pendant 8 heures sous obscurité et 36 o C pendant 16 heures sous lumière durant quatre semaines. La culture du bout du méristème combinée à la thermothérapie a perimis l'élimination de SPFMV et SPMMV dans 77 % des plants qui étaient au départ infectés avec des virus respectifs. Par ailleurs, l'élimination de SPCSV avait échoué.

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Section: Introductionmentioning

confidence: 99%

Detection and elimination of sweetpotato viruses

Rukarwa

Mashingaidze²,

Kyamanywa³

et al. 2011

African Crop Science Journal

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“…During the past decade, it has become apparent that fungus-transmitted, bipartite viruses, such as barley yellow mosaic virus (BaYMV), share an evolutionary relationship with the aphid-transmitted potyviruses (20,23) and are members of the genus Bymovirus (the type species is BaYMV) within the family Potyviridae. The whitefly-transmitted sweet potato mild mottle virus (SPMMV) is clearly a member of the family Potyviridae, and the genus "Ipomovirus" has been proposed, with SPMMV as the sole species proposed (12). Thus, for these three genera of the family Potyviridae, sequence comparisons have validated species affiliations within genera that group virus species transmitted by the same vector taxa.…”

mentioning

confidence: 99%

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

Stenger¹,

Hall²,

Choi³

et al. 1998

Phytopathology®

125

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The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5′ leader and 149-nt 3′-untranslated region and is polyadenylated at the 3′ end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5′-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3′). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is the type species. Phylogenetic analyses were conducted with complete polyprotein or NIb protein sequences of 11 members of the family Potyviridae, including viruses of monocots or dicots and viruses transmitted by aphids, whiteflies, and mites. WSMV and the monocot-infecting, mite-transmitted brome streak mosaic virus (BrSMV) are sister taxa and share a most recent common ancestor with the whitefly-transmitted sweet potato mild mottle virus, the type species of the proposed genus “Ipomovirus.” In contrast, RGMV shares a most recent common ancestor with aphid-transmitted species of the genus Potyvirus. These results indicate that WSMV and BrSMV should be classified within a new genus of the family Potyviridae and should not be considered species of the genus Rymovirus.

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“…Although no séquence information was available for viruses taxonomically related to SPMMV, thé particular problems associated with this virus were overcome by designing broad spectrum PCR primers derived from conserved régions among members of thé family Potyviridae (Colinet et al, 1996) and using thé RACE procédure (Frohman, 1990). The strategy employed to amplify thé complète SPMMV genomic RNA is shown in Fig.…”

Section: Cloning Of Spmmv Rna Génomementioning

confidence: 99%

“…However, thé zinc-fmger motif, which is suspected to be involved in aphid transmission of potyviruses, is not complète in SPMMV. Furthermore, it is noteworthy that thé motif DAG, involved in aphid transmission of potyviruses, is not found in thé coat protein of SPMMV (Colinet et al, 1996). Mutagenesis studies would help to détermine if such modifications in SPMMV HC-Pro and CP reflect its transmission by a différent vector.…”

Section: Comparison Of Proposed Spmmv Mature Proteins With Those Of Omentioning

confidence: 99%

“…Recently, we reported thé séquence of «2100 nucleotides derived from thé 3' end of thé SPMMV génome by family-specific RT-PCR primers (Colinet et al, 1996). Limited séquence identity in thé coat protein core with other members of thé family Potyviridae suggested that SPMMV should be assigned to a distinct genus (Colinet et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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The nucleotide sequence and genome organization of the whitefly transmitted sweetpotato mild mottle virus: a close relationship with members of the family Potyviridae

1998

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Abs tractPrimers corresponding to conservée! régions in thé RNA-dependent RNA polymerase and thé RACE procédure led to thé cloning of thé complète sweetpotato mild mottle virus (SPMMV) RNA génome. The assembled SPMMV genomic séquence was 10818 nucleotides in length with a polyadenylated tract at thé 3' terminus. The structure and organization of thé SPMMV génome appear to be similar to those of potyviruses and rymoviruses. A 5' untranslated région, rich in A and U residues, is présent between nucleotides 1 and 139. A putative initiation codon, at nucleotides 140-142, marks thé beginning of a large open reading frame (ORF) which ends in UAA at positions 10 508-10 510. A 308-nucleotide untranslated région is présent between thé termination codon of thé ORF and thé beginning of thé 3' polyadenylated région. Almost ail known potyvirus motifs are présent in thé polyprotein of SPMMV. However, motifs in thé putative helper-component and coat protein of SPMMV are incomplète or missing, which may account for ils vector relations. Despite similarities with rymoviruses, potyviruses and, to a lesser extent, bymoviruses, comparative séquence analyses demonstrated that SPMMV belongs to a distinct genus of thé family Potyviridae.

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Molecular evidence that the whitefly-transmitted sweetpotato mild mottle virus belongs to a distinct genus of thePotyviridae

Cited by 37 publications

References 32 publications

Detection and elimination of sweetpotato viruses

Detection and elimination of sweetpotato viruses

Phylogenetic Relationships Within the Family Potyviridae: Wheat Streak Mosaic Virus and Brome Streak Mosaic Virus Are Not Members of the Genus Rymovirus

The nucleotide sequence and genome organization of the whitefly transmitted sweetpotato mild mottle virus: a close relationship with members of the family Potyviridae

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