“Molecular extracts” for allergy diagnostics and therapy

Matricardi, Paolo Maria; Dramburg, Stephanie; Skevaki, Chrysanthi; Renz, Harald

doi:10.1111/pai.13001

Cited by 12 publications

(14 citation statements)

References 32 publications

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“…Our results showed that although >70% of the patients were sensitized to any of the major allergens, a combination panel including mid-tier allergens like Der p 5, Der p 7, and Der p 21 enhanced identification up to >94% of the moderate-to-severe mite-sensitized asthmatics. The role of both major and mid-tier D. pteronyssinus allergens needs further investigation not only in terms of prevalence but also in the pathophysiology underlying the type 2 inflammation in moderate-to-severe asthma to promote a genuine diagnosis, leading to a tailored mite AIT in the near future [65]. The proposed CRD panel approach appears to be sufficient to obtain a precise D. pteronyssinus molecular diagnosis in moderate-to-severe asthmatics with a marked mite allergen exposure, thus providing an enhanced depiction of the Th2-high asthma endotype.…”

Section: Discussionmentioning

confidence: 99%

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

González‐Pérez

Poza‐Guedes

Pineda

et al. 2020

Int Arch Allergy Immunol

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Background: The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in the concurrent asthma phenotypes has not been determined. Objective: The aim of the present study lays on a component-resolved diagnosis (CRD) model to investigate the mite molecular signature in subjects with type-2 inflammation asthma. Methods: We selected patients showing a clinically relevant sensitization to HDMs with moderate-to-severe persistent asthma. Skin prick test (SPT) with standardized mite extracts, a broad customized CRD serum sIgE panel including 9 Dermatophagoides pteronyssinus allergens and the related protein allergenic characterization, was investigated in all serum samples. Results: Ninety out of 93 (96.77%) patients with a positive SPT to HDM showed a concordant sIgE (≥0.35 kUA/L) to the crude extract of D. pteronyssinus. Major allergens (Der p 2, Der p 23, and Der p 1) were present in >70% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 51% in the present cohort. A complex pleomorphic repertoire of HDM molecules recognized by IgE was depicted, including 38 distinct profiles. Conclusions and Clinical Relevance: The proposed CRD panel approach, containing the most prevalent HDM allergens, appeared to be sufficient to obtain a precise D. pteronyssinus molecular diagnosis in asthmatics with a climate-dependent high-mite allergen exposure and coexisting sensitization. A dominant role of both major and mid-tier allergens has been confirmed in moderate and severe persistent asthmatics with the preponderant Th2-high endotype.

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Section: Discussionmentioning

confidence: 99%

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

González‐Pérez

Poza‐Guedes

Pineda

et al. 2020

Int Arch Allergy Immunol

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“…Skin prick tests (SPTs) to wheat and oats (1:10 w/v 0.9% NaCl), and gliadin (1 mg/mL, Sigma) were performed as previously described in 14 of 15 children. 3 The median SPT reactions to oats, wheat, and gliadin were 7 mm, 10 mm, and 6 mm, respectively. One child had a negative oat SPT ( Table 1).…”

Section: Children With Wheat Allergy Usually Tolerate Oatsmentioning

confidence: 92%

“…Sensitization to gliadin predicts persistent wheat allergy and a slower course of recovery. 3 The gluten-containing cereals wheat, rye, and barley are taxonomically closely related, and their storage proteins display extensive sequence homology and IgE cross-reactivity. Oats belong to the same grass family but are more distantly related to wheat.…”

Section: Children With Wheat Allergy Usually Tolerate Oatsmentioning

confidence: 99%

“…It allows diagnosing genuine versus cross-reactive sensitization with high specificity and thus identifying patients for specific immunotherapy. 2,3 Furthermore, an emerging field of research deals with the establishment of serological parameters based on sensitization patterns which are associated with phenotypes of allergy. It was shown that in food allergic patients, the sensitization to food allergens with high stability, like the major peanut allergen Ara h 2, is associated with a higher risk of severe systemic reactions, while sensitization to food allergens which are less stable to heat and digestion is linked to local reactions.…”

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confidence: 99%

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Molecular allergy diagnosis: A potential tool for the assessment of severity of grass pollen‐induced rhinitis in children

Douladiris

Garib

Piskou

et al. 2019

Pediatric Allergy Immunology

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“…In this context, a product with HDM allergenic extract has been developed using Indonesian HDM (IHDM) to diagnose the sensitivity of patients to HDM allergen. The HDM allergenic extracts originating from different regions and prepared by different manufacturers are expected to have different allergenicity [10][11][12] and have been reported to induce a variety of T-cell responses and IgE binding due to different sequences of the allergenic protein, diversity of raw materials, and different compositions [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Indonesian house dust mite allergenic extract in triggering allergic rhinitis sensitivity in a mouse model: A preliminary study

et al. 2022

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Background and Aim: Perennial allergic rhinitis (AR) is a chronic upper respiratory disease, with inflammation mediated by immunoglobulin E in the nasal mucosa caused by house dust mites. Recently, allergen immunotherapy showed promising allergic healing in patients with a definite history of sensitization. Based on this finding, a product was developed using Indonesian house dust mite (IHDM). This study aimed to optimize the allergenic rhinitis mouse model that was generated using IHDM to test the in vivo sensitivity and safety of this product. Materials and Methods: Seven groups of mice were used for effectiveness testing – normal, negative control with IHDM challenge, positive control with 0.1% histamine challenge, and AR group by both IHDM-induced sensitization at 12.5, 50, 250, or 500 μg and IHDM challenge. Mice were sensitized by intraperitoneal administration of IHDM once a week for 3 consecutive weeks. Thereafter, the challenge was given intranasally 5 times on alternate days. The number of nose rubbing and sneezing was noted. Eosinophil infiltration was assessed histologically using hematoxylin and eosin staining. The expression of interleukin-5 (IL-5) mRNA in the nasal mucosa was determined using semi-quantitative reverse transcription-polymerase chain reaction. Results: The induction of AR with IHDM significantly increased the number of nose rubbing and sneezing in the mouse model. Eosinophil infiltration was observed in the nasal mucosa; however, no significant change occurred in the expression of IL-5 mRNA. Conclusion: Overall, these data indicate that IHDM allergenic extract could be an effective sensitizing agent in a mouse model of AR. Although the use of IHDM is a limitation of this study because other sources of house dust mites might have different effects, this study provides a proper model for immunotherapy effectivity testing for in vivo pre-clinical studies.

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“Molecular extracts” for allergy diagnostics and therapy

Cited by 12 publications

References 32 publications

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen Exposure

Molecular allergy diagnosis: A potential tool for the assessment of severity of grass pollen‐induced rhinitis in children

Effectiveness of Indonesian house dust mite allergenic extract in triggering allergic rhinitis sensitivity in a mouse model: A preliminary study

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