Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium

Glembotski, Christopher C.; Wildey, Gary; Gibson, Thomas R.

doi:10.1016/0006-291x(85)91944-8

Cited by 75 publications

(20 citation statements)

References 23 publications

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“…It not only exhibited the best displacement curve of antibody bound to 125 1 CDN 1-16 by unlabelled CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] but also demonstrated the highest cross-reactivity with endogenous pro-ANP in both human and rat. As the complete endogenous molecule (pro-ANP was not available synthetically formal cross-reactivity studies could not be performed, but this antiserum could detect the highest concentrations of cardiodilatin-IR and exhibited the best correlation with ANP concentrations, determined by the ANP radioimmunoassay, in the same human atrial extracts.…”

Section: Antiseramentioning

confidence: 99%

“…As the complete endogenous molecule (pro-ANP was not available synthetically formal cross-reactivity studies could not be performed, but this antiserum could detect the highest concentrations of cardiodilatin-IR and exhibited the best correlation with ANP concentrations, determined by the ANP radioimmunoassay, in the same human atrial extracts. The cross-reactivity of the antiserum with human synthetic CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] was 100% and with CDN 1-67 44%. The antiserum showed no cross-reactivity with alpha-human-ANP.…”

Section: Antiseramentioning

confidence: 99%

“…The iodinated CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] was eluted from the HPLC column by a concentration of 30% (v j v) acetonitrile in water containing 0.05% (v j v) trifluoroacetic acid, in a single radioactivity peak (specific activity of 60-70 Bqj fmol). In the antibody-free tube, non-specific binding was < 3%, zero standard binding was 45-50% and excess antibody binding was 85-90%.…”

Section: Iodinationmentioning

confidence: 99%

“…The 126 arnino acid gamma atrial natriuretic peptide, or pro-ANP, alpha-ANP consisting of the 28 arnino acid sequence of the C-terrninal of pro-ANP and beta-ANP which is an antiparallel dimer of alpha-ANP in the human and an N-terrninally extended alpha ANP in the rat [7]. A number of reports have appeared in the literature describing the pres en ce of N-terrninally extended and C-and N-terrninally truncated forms of alpha-ANP in atrial tissues [8][9][10][11][12][13][14], which are probably extraction and purification artefacts [5,15] but the presence of the N-terrninal peptide of pro-ANP, lacking alpha-ANP in its sequence,has not been reported to date.…”

Section: Introductionmentioning

confidence: 99%

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The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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A sensitive and specific radioimmunoassay has been developed for cardiodilatin; the N-terrninal peptide sequence of the atrial natriuretic peptide (ANP) prohorrnone. Cardiodilatin-immunoreactivity (-IR) concentrations in the human right atrial appendage we re found to correlate with ANP-IR concentrations, deterrnined by an established radioimmunoassay, (cardiodilatin-IR = 13.2 ± 1.2 nmoljg, ANP-IR = 19.8 ± 2.0 nmoljg, r = 0.80, p < 0.001). Characterisation of the cardiodilatin-IR in the human and rat right atrium by gel permeation and fast protein liquid chromatography revealed only two cardiodilatin-IR molecular forms. The larger more hydrophobic form, the majority of the cardiodÜatin-IR, contained in addition ANP-IR and therefore represents the prohorrnone. The smaller, less hydrophobic form, lacked ANP-IR and thus represents the cleaved N-terrninal peptide sequence of the prohorrnone. These findings indicate that the prohorrnone is the major molecular form in the human and rat atrium. Furthermore, they demonstrl:lte that a single large N-terrninal peptide, cardiodilatin, derived from the prohorrnone, may exist as a distinct molecular form in the atrium of these species.

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Section: Antiseramentioning

confidence: 99%

Section: Antiseramentioning

confidence: 99%

Section: Iodinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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“…Rat ANP-IV with the amino acid sequence H 2 N-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Ile-Asp-Arg-Ile-GlyAla-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr-COOH (Misono et al 1984a,b) was synthesized as previously described (Sugiyama et al 1984), coupled to thyroglobulin, and injected intradermally in Dutch belted rabbits (Tanaka et al 1984). ANP-IV is contained within the rat ANP 1-28 sequence and is identical to the low molecular weight form isolated from rat brain (Glembotski et al 1985;Shiono et al 1986). Antisera against synthetic ANP-IV crossreacted 100% with natural ANP-IV, 100% with rat ANP-II, 42.5% with human ANP ( ␣ hANP 4-28 ), and 28% with atriopeptin I, but did not crossreact with arginine-vasopressin, angiotensin I (AI), or angiotensin II (AII) (McKenzie et al 1985).…”

mentioning

confidence: 99%

Atrial Natriuretic Peptide-like Immunoreactivity in Neurons and Astrocytes of Human Cerebellum and Inferior Olivary Complex

McKenzie

Juan

Thomas

et al. 2001

J Histochem Cytochem.

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S U M M A R Y Atrial natriuretic peptide (ANP) has previously been localized in areas of mammalian brain associated with olfaction, cardiovascular function, and fluid/electrolyte homeostasis. Despite the presence of several types of natriuretic peptide receptors in mammalian cerebellum, neither intrinsic nor extrinsic sources of the natriuretic peptides have been described. In this report we describe the immunohistochemical localization of both intrinsic and extrinsic sources for ANP in human cerebellum. ANP-like immunoreactivity (ANP-LIR) was observed in climbing fibers in the cerebellar molecular layer that probably originated from isolated immunopositive neurons of the inferior olivary complex. Intrinsic sources of ANP-LIR included small subpopulations of protoplasmic and fibrous astrocytes and Bergmann glia, as well as Golgi and Lugaro neurons of the granule cell layer. These results suggest that, in addition to its presumptive roles in local vasoregulation, ANP may serve as a modulator of the activity of Purkinje neurons.

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Organization of atriopeptin‐like immunoreactive neurons in the central nervous system of the rat

Standaert

Needleman

Saper

1986

J of Comparative Neurology

192

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The atrial natriuretic peptide, atriopeptin, is a circulating hormone that plays an important role in the regulation of fluid and electrolyte homeostasis. Several recent studies have shown that atriopeptin-like immunoreactivity is present within the central nervous system as well as peripheral tissues. In the present report, we describe in detail the organization of atriopeptin-like immunoreactive (APir) perikarya and fibers in the central nervous system of the rat. The most prominent collection of APir perikarya was found in the hypothalamus, adjacent to the anteroventral tip of the third ventricle. Additional groups of APir perikarya were observed along the wall of the third ventricle and in the paraventricular and arcuate nuclei. Separate, smaller groups with distinctive morphology were seen in the lateral hypothalamic area, in the supra-mammillary, medial, and lateral mammillary nuclei, medial habenular nucleus, bed nucleus of the stria terminalis, and the central nucleus of the amygdala. In the pons and brain-stem, APir neurons were observed in the pedunculopontine and laterodorsal tegmental nuclei, as well as in the ventral tegmental area, Barrington's nucleus, the parabrachial nucleus, and the nucleus of the solitary tract. The densest terminal fields of APir fibers were found in the paraventricular nucleus of the hypothalamus, the bed nucleus of the stria terminalis, the median eminence, and the interpeduncular nucleus. The presence of atriopeptin immunoreactivity within the central nervous system suggests that atriopeptin may function as a central neuromediator. Potential functions of this candidate neuromediator deduced from its anatomical distribution are discussed, including the possibility that atriopeptin may function as both a central neuromediator and a systemic hormone in the regulation of the cardiovascular system.

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Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium

Cited by 75 publications

References 23 publications

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Atrial Natriuretic Peptide-like Immunoreactivity in Neurons and Astrocytes of Human Cerebellum and Inferior Olivary Complex

Organization of atriopeptin‐like immunoreactive neurons in the central nervous system of the rat

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