Molecular genetic characterization and haplotype diversity of swine leukocyte antigen in Chinese Rongshui miniature pigs

Gao, Caixia; Xin, Chang; Wang, Xiuying; Quan, Jinqiang; Li, Changwen; Wang, Jingyu; Chen, Hongyan

doi:10.1016/j.molimm.2019.05.009

Cited by 8 publications

(13 citation statements)

References 37 publications

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“…The frequency and diversity of SLA alleles have already been characterized in several purebred populations including, Canadian Yorkshire and Landrace (8), Chinese Bama miniature pig (9), Duroc (10), German Landrace (11), Gottingen minipigs (12), Guizhou minipigs (13), Korean native pig (14), Meishan (15), Microminipig (16), Pietrain (17), Yucatan miniature pig (18), and recently, Babraham (19) and Rongshui miniature pigs (20). However, almost all of these studies were conducted in populations which were purposely bred as a resource for scientific experimentation.…”

Section: Introductionmentioning

confidence: 99%

“…Despite being the majority, information on SLA diversity in these crossbred populations are rather scarce, with studies conducted in the mixed US herds (50%LY/50%D and 50%L/50%Y) (21,22), Danish herds (50%LY/50%D) (12,23) and Belgian herds (50%L/50%P) (12). Moreover, almost all SLA class II diversity studies were in the purebred animals (8,10,13,15,(18)(19)(20), with only one conducted in the US outbred population (22).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

et al. 2021

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Among swine genetic markers, the highly polymorphic swine leukocyte antigen (SLA) is one of the key determinants, associated with not only immune responses but also reproductive performance and meat quality. The objective of this study was to characterize the SLA class I and II diversities in the commercial pig populations. In this study, a total number of 158 pigs (126 gilts and 32 boars) were randomly selected from different breeding herds of five major pig-producing companies, which covered ~70% of Thai swine production. The results indicate that a moderate level of SLA diversity was maintained in the Thai swine population, despite the performance-oriented breeding scheme. The highly common SLA class I alleles were SLA-1*08:XX, SLA-2*02:XX, and SLA-3*04:XX at a combined frequency of 30.1, 18.4, and 34.5%, respectively, whereas DRB1*04:XX, DQB1*02:XX and DQA*02:XX were the common class II alleles at 22.8, 33.3, and 38.6%, respectively. The haplotype Lr-32.0 (SLA-1*07:XX, SLA-2*02:XX, and SLA-3*04:XX) and Lr-0.23 (DRB1*10:XX, DQB1*06:XX, DQA* 01:XX) was the most common SLA class I and II haplotype, at 15.5 and 14.6%, respectively. Common class I and II haplotypes were also observed, which Lr-22.15 was the most predominant at 11.1%, followed by Lr-32.12 and Lr-4.2 at 10.8 and 7.9%, respectively. To our knowledge, this is the first report of SLA class I and II diversities in the commercial pigs in Southeast Asia. The information of the common SLA allele(s) in the population could facilitate swine genetic improvement and future vaccine design.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

et al. 2021

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“…Given the strong linkage disequilibrium exhibited by the SLA loci, it is sometimes more appropriate and convenient for researchers to communicate and present findings in terms of haplotypes rather than individual allele specificities [ 4 ]. To the best of our knowledge, at least 91 SLA class I haplotypes and 47 SLA class II haplotypes have been submitted to the ISAG SLA Nomenclature Committee [ 29 ]. A variety of molecular methods have been described for typing SLA alleles, including reverse transcription PCR (RT-PCR) sequence-based typing, PCR-sequence-specific primers (PCR-SSP), PCR-restriction fragment length polymorphism (PCR-RFLP), and MS.…”

Section: Discussionmentioning

confidence: 99%

“…A variety of molecular methods have been described for typing SLA alleles, including reverse transcription PCR (RT-PCR) sequence-based typing, PCR-sequence-specific primers (PCR-SSP), PCR-restriction fragment length polymorphism (PCR-RFLP), and MS. Many SLA class I and II haplotypes have been identified in different pig breeds and porcine cell lines using these methods [ 29 – 31 ]. Among them, the MS marker technique is a fast and cost-efficient method for molecular SLA typing [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Many phenotypic characteristics of Mingguang small-ear pigs are also between those of the two types; therefore, phenotypic characteristics , and theyit areis not suitable forto classifyingclassify Mingguang small-ear pigs based on phenotypic characteristics. Therefore, some scholars have classified Mingguang small-ear pigspig as a plateau tTtransition-type[28].However, another scholars have questioned the view that Mingguang small-ear pigs have become a new strain independently of Diannan small-ear pigs[29]. The results showed that the genetic distance between Mingguang small-ear pigspig and Diannan small-ear pigspig was the smallestwere the closest, suggesting that they have a very similarremarkably similar genetic backgrounds and originate from the same population.…”

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Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites

Liu

et al. 2021

Anim Biosci

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Background: The sSwine leukocyte antigen (SLA) gene group, which is, a closely linked and highly polymorphic gene group, has important biomedical significance in the protection and utilization of germplasm resources. However, studiesthe study onof genetic polymorphismpolymorphisms analysesis of SLA microsatellite markers (MS) in Chinese miniature pigs areis limited. Methods: To study the genetic diversity of Chinese miniature pigs, eEighteen pairs of microsatellite primers were used designed to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from in different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The geneticGenetic differentiation coefficient (Fst) and neighbor-joining (NJ) methodsmethod were used for cluster analysis ofamong the breeds. Results: In miniature pigs, tThe results showed that Thethe microsatellite polymorphism of the SLA I region had the was the highest numbers of polymorphisms,, followed by the SLA II and SLA III regions in the miniature pigs;, and the region near the centromere had the lowest number of polymorphismsshowed the lowest polymorphism. Among the seven miniature pig breeds, Diannan small-ear pigspig had the highest genetic diversity (PIC value = 0.6396), whereasile the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst valuesvalue

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A comparative analysis of SLA-DRB1 genetic diversity in Colombian (creoles and commercial line) and worldwide swine populations

Celis-Giraldo

Bohórquez

Camargo

et al. 2021

Sci Rep

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Analysing pig class II mayor histocompatibility complex (MHC) molecules is mainly related to antigen presentation. Identifying frequently-occurring alleles in pig populations is an important aspect to be considered when developing peptide-based vaccines. Colombian creole pig populations have had to adapt to local conditions since entering Colombia; a recent census has shown low amounts of pigs which is why they are considered protected by the Colombian government. Commercial hybrids are more attractive regarding production. This research has been aimed at describing the allele distribution of Colombian pigs from diverse genetic backgrounds and comparing Colombian SLA-DRB1 locus diversity to that of internationally reported populations. Twenty SLA-DRB1 alleles were identified in the six populations analysed here using sequence-based typing. The amount of alleles ranged from six (Manta and Casco Mula) to nine (San Pedreño). Only one allele (01:02) having > 5% frequency was shared by all three commercial line populations. Allele 02:01:01 was shared by five populations (around > 5% frequency). Global FST indicated that pig populations were clearly structured, as 20.6% of total allele frequency variation was explained by differences between populations (FST = 0.206). This study’s results confirmed that the greatest diversity occurred in wild boars, thereby contrasting with low diversity in domestic pig populations.

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Molecular genetic characterization and haplotype diversity of swine leukocyte antigen in Chinese Rongshui miniature pigs

Cited by 8 publications

References 37 publications

Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

Genetic diversity analysis in Chinese miniature pigs using swine leukocyte antigen complex microsatellites

A comparative analysis of SLA-DRB1 genetic diversity in Colombian (creoles and commercial line) and worldwide swine populations

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