Molecular genetic studies on Nellore and Deccani sheep using microsatellite markers

Abstract

The study was undertaken to understand the genetic architecture of Deccani and Nellore sheep breeds and to assess the genetic distance between the breeds. Allele diversity, genetic variability and population structure in the two breeds were estimated using 30 microsatellite markers. A total of 100 sheep, 50 each from Deccani and Nellore breeds were genotyped. The total number of alleles observed was 254 and 260 in Deccani and Nellore, respectively. The mean expected heterozygosity among the breeds ranged from 0.79 (Deccani) to 0.80 (Nellore) whereas the mean observed heterozygosity levels ranged from 0.77 in Deccani to 0.78 in Nellore sheep. The overall mean inbreeding coefficient was 0.029 in Deccani and 0.031 in Nellore sheep and of the 30 loci studied, 14 loci in Deccani and 16 loci in Nellore sheep showed negative inbreeding coefficient indicating the presence of outbreeding. However, eight loci (BM1314, BM6506, BM8125, ILSTS28, MAF70, MCM140, OarCB226 and OarFCB128) showed positive, mild to moderate inbreeding ranging from 0.007 to 0.25 in Deccani and 0.066 to 0.37 in Nellore sheep studied. The F IS values ranged from -0.372 to 0.74, F ST values ranged from 0.001 to 0.172 and F IT values ranged from -0.370 to 0.728 between the Deccani and Nellore sheep breeds studied. The PIC values ranged from 0.498 to 0.886 with a mean of 0.773 in Deccani and from 0.574 to 0.894 with a mean of 0.777 in Nellore breed. The information generated from the present study will be valuable for setting conservation priority of these two breeds.

Genetic diversity analysis of native sheep population of Eastern Indian Himalayas using microsatellite DNA markers

“…Six loci each in Assam and Meghalaya sheep and eight loci in Arunachal Pradesh sheep were found to be monomorphic for which no subsequent analysis was done. Similar reports of 242 alleles scored in different sheep breed was reported by Amareswari et al (2018) in Deccani (254 alleles) and Nellore sheep breed (260 alleles) and Molabanti et al (2019) in Macherla Brown sheep breed (275 alleles). However, lower total alleles scored by Ravimurugan (2015) in Madras Red sheep breed (175 alleles) and Martínez et al (2021) in Mexican Pelibuey sheep population (99 alleles); and a higher total allele was scored in Deccan sheep (407 alleles) by Yadav et al (2017).…”

“…A relatively similar finding for number of alleles was reported by Arora et al(2008) and Pramod et al (2009) in Jalauni and Vembur sheep breeds which ranged from two to nine. However, a higher range was reported by Amareswari et al (2018) The observed and effective numbers of alleles are also an index used to reveal the genetic diversity of the populations. The mean observed numbers of alleles (N a ) were found to be 3.160, 3.360 and 3.160 in Assam, Meghalaya and Arunachal Pradesh sheep respectively.…”

Genetic admixture and diversity in Thai domestic chickens revealed through analysis of Lao Pa Koi fighting cocks

“…By contrast, a remarkable difference between H o and H e was observed for LPK chicken population, where H o was higher than H e , and there was no subpopulation within LPK chicken population examined (negative F value). This higher heterozygosity than expected may reflect that genetic contamination, such as outbreeding with individuals from populations with different origins or ecotypes, occurred during the process of breeding in the past [ 50 , 51 ]. In the Lamphun region, a suitable environment for both LPK chickens and red junglefowl is provided by the forest.…”

The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

“…Further research can be carried out by comparing samples from other regions in Indonesia and from neighboring countries [ 40 ]. Apart from studying part of mtDNA, more detailed studies can also be carried out such as microsatellite [ 41 , 42 ], complete mitochondrial genome [ 27 , 43 ], whole-genome DNA [ 44 , 45 ], and the other studies using the next-generation sequencing [ 46 ] and/or the other methods.…”