Molecular genetics and the initiation of solventogenesis inClostridium beijerinckii(formerlyClostridium acetobutylicum) NCIMB 8052

Abstract

A physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome has been constructed, encompassing about 90 rare restriction sites. The 14 rrn operons together with about 40 genes have been assigned positions on the map. Genetic analysis and gene transfer have been developed in this organism to enable in vivo analysis of the roles of cloned genes using marker replacement technology. Experiments using the available genetic tools have shown that spo0A plays a cardinal role in controlling several aspects of the transition from exponential growth to stationary phase in C. beijerinckii. These include initiation of sporulation, accumulation of the storage polysaccharide, granulose, and production of acetone and butanol. Several C. beijerinckii and C. acetobutylicum genes concerned with fermentative metabolism, whose expression is modulated at the onset of solventogenesis, contain sequence motifs resembling 0A boxes in their 5' regulatory regions. This invites the speculation that they are under the direct control of Spo0A, and additional data are now required to test this prediction.

Genome-wide dynamic transcriptional profiling in clostridium beijerinckii NCIMB 8052 using single-nucleotide resolution RNA-Seq

“…Comparatively, the transcription level of spo0A was much higher than that of spo0H (Figure 4B). Putative σ H -dependent promoter and Spo0A-binding motif (0A box) have been identified upstream of the spo0A gene in C. beijerinckii 8052 [35]. …”

Metabolic Engineering of Histidine Kinases in Clostridium beijerinckii for Enhanced Butanol Production

“…Cac3319, Cac0903, and Cac0323 are essential for Spo0A activation, which controls the initiation of sporulation and solventogenesis (Steiner et al, 2011;Xu et al, 2015). The "0A box" that can be bound with Spo0A∼P has been proved present in the upstream regions of lots of genes whose expressions are either activated or repressed at the initiation of solventogenesis in both C. acetobutylicum and C. beijerinckii (Wilkinson et al, 1995). Generally, the sporulating phenotype is considered to be profitable for solvent formation, but solvent production ceases when mature spores form (Kolek et al, 2016).…”

“…Especially, orphan HKs directly phosphorylate Spo0A, which is the master regulator of both sporulation and solventogenesis in C. acetobutylicum (Steiner et al, 2011). Spo0A directly controls the genes with the presence of one or more "0A boxes" (TGNCGAA) in their 5 regulatory regions (Wilkinson et al, 1995). The "0A boxes" are present in the upstream regions of many genes involving sporulation such as sigE and spoIIE, and central metabolism such as adc, sol operon, bdhA and bdhB (Wilkinson et al, 1995;Kolek et al, 2017).…”

“…Spo0A directly controls the genes with the presence of one or more "0A boxes" (TGNCGAA) in their 5 regulatory regions (Wilkinson et al, 1995). The "0A boxes" are present in the upstream regions of many genes involving sporulation such as sigE and spoIIE, and central metabolism such as adc, sol operon, bdhA and bdhB (Wilkinson et al, 1995;Kolek et al, 2017). To regulate the transcription of these genes, the phosphorylated Spo0A will bind to the "0A boxes" to function.…”

Continuous Butanol Production Using Suspended and Immobilized Clostridium beijerinckii NCIMB 8052 with Supplementary Butyrate

“…Clostridium beijerinckii NCIMB 8052 is one of the most common solventogenic clostridia studied – and the parental strain of C. beijerinckii BA101, a solvent-hyperproducing mutant …”