Molecular hydrogen ameliorates several characteristics of preeclampsia in the Reduced Uterine Perfusion Pressure (RUPP) rat model

Ushida, Takafumi; Kotani, Tomomi; Tsuda, Hiroyuki; Imai, Kenji; Nakano, Tomoko; Hirako, Shima; Ito, Yukihiro; Li, Hua; Mano, Yukio; Wang, Jingwen; Miki, Rika; Yamamoto, Eiko; Iwase, Akira; Bando, Yoshimi; Hirayama, Masaaki; Ohno, Kinji; Toyokuni, Shinya; Kikkawa, Fumitaka

doi:10.1016/j.freeradbiomed.2016.10.491

Cited by 29 publications

(27 citation statements)

References 57 publications

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“…Accordingly, hydrogen is now believed to reduce oxidative stress and ischemia-reperfusion injury in the brain, spinal cord (10), myocardium (11), intestinal epithelium (12), retina, testes (13) and kidneys (14). Hydrogen has been used to treat various states associated with oxidative stress, including trauma (15), neurodegenerative disease (16), inflammatory disease (17), organ transplantation, metabolic syndrome (18), diabetes mellitus (19), sepsis (20), burns (21), adverse reactions to chemotherapy (22), radiation injury (23), hearing disorders and preeclampsia (24). There are several studies on the preventive and therapeutic effects of hydrogen on various diseases, including cancer (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen gas restores exhausted CD8+ T�cells in patients with advanced colorectal cancer to improve prognosis

Akagi

Baba

2018

Oncol Rep

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Exhausted cluster of differentiation (CD)8 + T cells lose immunological activity due to mitochondrial dysfunction caused by peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) inactivation, resulting in a poor prognosis in patients with cancer. As hydrogen gas was recently reported to activate PGC-1α, the present study investigated whether it restores exhausted CD8 + T cells to improve prognosis in patients with stage IV colorectal cancer. A total of 55 patients with histologically and clinically diagnosed stage IV colorectal carcinoma were enrolled between July 2014 and July 2017. The patients inhaled hydrogen gas for 3 h/day at their own homes and received chemotherapy at the Tamana Regional Health Medical Center (Tamana, Kumamoto, Japan). The CD8 + T cells were isolated from the peripheral blood and their phenotype was analyzed by flow cytometry. It was found that exhausted terminal programmed cell death 1 (PD-1) + CD8 + T cells in the peripheral blood are independently associated with worse progression-free survival (PFS) and overall survival (OS). Notably, hydrogen gas decreased the abundance of exhausted terminal PD-1 + CD8 + T cells, increased that of active terminal PD-1 -CD8 + T cells, and improved PFS and OS times, suggesting that the balance between terminal PD1 + and PD1 -CD8 + T cells is critical for cancer prognosis. Therefore, a novel system for patient classification (category 1-4) was developed in the present study based on these two indices to assist in predicting the prognosis and therapeutic response.Collectively, the present results suggested that hydrogen gas reverses imbalances toward PD-1 + CD8 + T cells to provide an improved prognosis.

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Section: Introductionmentioning

confidence: 99%

Hydrogen gas restores exhausted CD8+ T�cells in patients with advanced colorectal cancer to improve prognosis

Akagi

Baba

2018

Oncol Rep

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“…Preeclampsia (PE) is a pregnancy-specific hypertensive syndrome affecting ~2-7% of pregnant women globally, developing subsequent to 20 weeks of gestational age (1). The clinical manifestations of PE include hypertension, placental hypoxia, proteinuria, endothelial dysfunction, end-organ ischemia and increased vascular permeability (2).…”

Section: Introductionmentioning

confidence: 99%

Regulation of steroid hormones in the placenta and serum of women with preeclampsia

et al. 2017

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Preeclampsia (PE) is a pregnancy‑specific hypertensive syndrome that results in substantial maternal and fetal morbidity and mortality. The exact cause of PE has not been completely elucidate, although abnormal formation of the placenta has been considered. The placenta connects the developing fetus to the uterine wall, producing a large quantity of steroid hormones to maintain pregnancy. Although steroid hormones, particularly progesterone (P4) and estrogen (E2), in the serum of women with PE have been studied, steroidogenesis in the placenta has not well been established. The present study compared the concentrations of steroid hormones, including pregnenolone (PG), P4, dehydroepiandrosterone (DHEA), testosterone (T) and E2, in the serum and placenta of women with PE. PG, P4, DHEA and E2 concentrations tended to be decreased in PE serum and placentas, and the results were statistically significant for P4 and E2 in the serum. Quantification of genes associated with steroidogenesis in the placenta was performed, and the expression of the P4‑ and E2‑synthesizing enzymes testosterone 17‑β‑dehydrogenase 3 and 3 β‑hydroxysteroid dehydrogenase/δ5 4‑isomerase type 1 was reduced. Notably, aromatase, an enzyme required for the production of E2, was upregulated in the PE placenta, suggesting that steroidogenic enzymes may be dynamically regulated and may affect the symptoms of PE. In conclusion, the results of the present study suggested that the levels of steroid hormones, including P4 and E2, in the serum and placenta of women with PE are downregulated, which may be mediated by the regulation of steroidogenic enzyme expression in the PE placenta.

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“…En la PAD sólo se observaron diferencias entre los grupos RPPU y control. Ushida et al (2016) reportó en su investigación un aumento de las presiones arteriales sistólica y diastólica en modelos de ratas RPPU las cuales fueron medidas el día 19 postconcepción. Esto resulta interesante, pues en la mayoría de los estudios que utilizan modelos de ratas preeclámpticas sólo se mide la PAM porque revela cómo están siendo perfundidos los órganos corporales, pero un aumento de la PAS y la PAD podrían indicar que hay una disminución de la complacencia de la aorta y un aumento de la resistencia vascular periférica, muy similar a lo que ocurre durante la preeclampsia (Brennan et al, 2014).…”

Section: Discussionunclassified

Efectos de la Aspirina en Fetos de Rata con Reducción de la Presión de la Perfusión Uterina

et al. 2019

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CONEI, D.; CÁCERES, G. D.; HAVERBECK, M.; FIERRO, R.; VEUTHEY, C.; DEL SOL, M. & ROJAS, R. M.Efectos de la aspirina en fetos de rata con reducción de la presión de la perfusión uterina. Int. J. Morphol., 37(2): 739-743, 2019. RESUMEN: La preeclampsia (PE) es un trastorno hipertensivo inducido por el embarazo donde se reduce la presión de la perfusión uterina. Investigaciones avalan el uso de dosis baja de aspirina (DBAAS) y su utilidad en la prevención de PE en gestantes con factores de riesgo. Sus beneficios en modelos animales sometidos a esta reduccción no están determinados. El objetivo de la investigación fue analizar la presión arterial sistémica y los hallazgos morfológicos a nivel renal en fetos de ratas con reducción de la presión de perfusión uterina (RPPU) expuestas a DBAAS en comparación a las no expuestas. Se conformaron cuatro grupos de ratas hembras preñadas Sprague Dawley (n=5). A los 14,5 días post-concepción (dpc), vía quirúrgica se indujo RPPU, ligando arterias uterinas, conformándose el grupo RPPU y el grupo RPPU+DBAAS al que se le administró 5 mg/kg/día de aspirina vía oral. El grupo control lo conformaron las no operadas y el grupo DBAAS se le administró aspirina en igual dosis desde el 14,5 dpc. A los 18,5 dpc, previo a la eutansia se midió la presión arterial sistémica con pletismógrafo caudal Insight v2.11 y se extrajeron los fetos. Se midió la longitud céfalo-caudal (LCC), se procesaron y tiñeron con hematoxilina-eosina, describiéndose cortes histológicos transversales a nivel renal. Se determinó que en la presión arterial media, hubo diferencias significativas entre el grupo RPPU y RPPU+DBAAS (p<0,05). El tamaño de los fetos fue menor en el grupo RPPU (p<0,0001), donde 1 feto presentó hernia umbilical congénita. La cuantificación de vesículas renales también fue menor (p<0,005). En conclusión, la administración de DBAAS disminuye los efectos inducidos por la RPPU en cuanto al tamaño fetal, morfología renal y malformaciones congénitas como hernia umbilical. En cuanto a la presión arterial sistémica, tendría efectos sólo en presión arterial media. PALABRAS CLAVE: Preeclampsia; Hipertensión inducida por el embarazo; Presión arterial; Aspirina. CONEI, D.; CÁCERES, G. D.; HAVERBECK, M.; FIERRO, R.; VEUTHEY, C.; DEL SOL, M. & ROJAS, R. M. Effects of aspirin on rat fetuses with reduction of uterine perfusion pressure. Int. J. Morphol., 37(2):739-743, 2019 SUMMARY: Preeclampsia (PE) is a hypertensive disorder induced by pregnancy where there is a reduction in the uterine perfusion pressure. Research supports the use of low dose aspirin (LDAAS) and CONEI, D.; CÁCERES, G. D.; HAVERBECK, M.; FIERRO, R.; VEUTHEY, C.; DEL SOL, M. & ROJAS, R. M.Efectos de la aspirina en fetos de rata con reducción de la presión de la perfusión uterina. Int. J. Morphol., 37(2):739-743, 2019.

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Molecular hydrogen ameliorates several characteristics of preeclampsia in the Reduced Uterine Perfusion Pressure (RUPP) rat model

Cited by 29 publications

References 57 publications

Hydrogen gas restores exhausted CD8+ T�cells in patients with advanced colorectal cancer to improve prognosis

Hydrogen gas restores exhausted CD8+ T�cells in patients with advanced colorectal cancer to improve prognosis

Regulation of steroid hormones in the placenta and serum of women with preeclampsia

Efectos de la Aspirina en Fetos de Rata con Reducción de la Presión de la Perfusión Uterina

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