Molecular identification and functional analysis of MyD88 in giant freshwater prawn (Macrobrachium rosenbergii) and expression changes in response to bacterial challenge

Gao, Qiang; Tang, Qisheng; Xia, Zhenglong; Yi, Shaokui; Cai, Miuying; Du, Houkuan; Yang, Jie; Jing-fen, LI; Xing, Qianqian; Luo, Jinping; Yang, Guoliang

doi:10.1016/j.ijbiomac.2021.02.177

Cited by 9 publications

(1 citation statement)

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“…Relish, an NF-κB transcription factor, also plays a key role in the innate immunity of crustaceans [ 80 ]. In crustaceans, the TLR2-MyD88 pathway regulates the immune response to pathogenic bacterial infections [ 81 , 82 ]. A transcriptomic analysis revealed that Cu 2+ exposure significantly affects the TLR pathway in Mizuhopecten yessoensis [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Feng

Song

et al. 2022

Antioxidants

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High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.

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