Molecular identification and morphological characteristics of native and invasive Asian brush-clawed crabs (Crustacea: Brachyura) from Japanese and German coasts: Hemigrapsus penicillatus (De Haan, 1835) versus Hemigrapsus takanoi Asakura &amp; Watanabe 2005

Markert, Alexandra; Raupach, Michael J.; Segelken-Voigt, Alexandra; Wehrmann, Achim

doi:10.1007/s13127-014-0176-4

Cited by 27 publications

(13 citation statements)

References 39 publications

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“…Following the guidelines of Collins & Cruickshank (2012), we aim at (i) assembling a DNA COI library from specimens identified morphologically to species level, (ii) testing the efficacy of this DNA library with simulations of sequence-based specimen identifications and (iii) exploring the possible causes of incongruence between morphological and sequence-based identifications. This study is part of the ongoing molecular characterization of the North Sea marine fauna conducted at the German Centre for Marine Biodiversity Research of the Senckenberg Institute (Laakmann et al 2013;Knebelsberger & Thiel 2014;Markert et al 2014;Vogt et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification ofNorthSea molluscs withDNAbarcoding

Barco

Raupach

Laakmann

et al. 2015

Molecular Ecology Resources

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Sequence-based specimen identification, known as DNA barcoding, is a common method complementing traditional morphology-based taxonomic assignments. The fundamental resource in DNA barcoding is the availability of a taxonomically reliable sequence database to use as a reference for sequence comparisons. Here, we provide a reference library including 579 sequences of the mitochondrial cytochrome c oxidase subunit I for 113 North Sea mollusc species. We tested the efficacy of this library by simulating a sequence-based specimen identification scenario using Best Match, Best Close Match (BCM) and All Species Barcode (ASB) criteria with three different threshold values. Each identification result was compared with our prior morphology-based taxonomic assignments. Our simulation resulted in 87.7% congruent identifications (93.8% when excluding singletons). The highest number of congruent identifications was obtained with BCM and ASB and a 0.05 threshold. We also compared identifications with genetic clustering (Barcode Index Numbers, BINs) computed by the Barcode of Life Datasystem (BOLD). About 68% of our morphological identifications were congruent with BINs created by BOLD. Forty-nine sequences were clustered in 16 discordant BINs, and these were divided in two classes: sequences from different species clustered in a single BIN and conspecific sequences divided in more BINs. Whereas former incongruences were probably caused by BOLD entries in need of a taxonomic update, the latter incongruences regarded taxa requiring further investigations. These include species with amphi-Atlantic distribution, whose genetic structure should be evaluated over their entire range to produce a reliable sequence-based identification system.

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Section: Introductionmentioning

confidence: 99%

Identification ofNorthSea molluscs withDNAbarcoding

Barco

Raupach

Laakmann

et al. 2015

Molecular Ecology Resources

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“…Therefore, integrating genetics and ecology is crucial in the context of biological invasions. Insights from these fields, combined with the understanding of evolutionary processes, could determine whether an invasion may take place, succeed, or fail (Sakai et al 2001, Lee 2002, Lawson Handley et al 2011, Seebens et al 2013, Markert et al 2014. Molecular identification and population genetics render new and exciting tools to improve our understanding of the population dynamics during biological invasions and have already shed light on the management, prevention and restoration strategies (Holland 2000, Sakai et al 2001, Le Roux & Wieczorek 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the non-native crab Rhithropanopeus harrisii (Brachyura: Panopeidae) in the Polish coastal waters − an example of patchy genetic diversity at a small geographic scale

Hegele-Drywa

Thiercelin

Schubart

et al. 2015

Oceanological and Hydrobiological Studies

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I n t e r n a t i o n a l J o u r n a l o f O c e a n o g r a p h y a n d H y d r o b i o l o g y by The Oceanological and Hydrobiological Studies is online at oandhs.ocean.ug.edu.pl pages (305-315) AbstractThe American panopeid crab species Rhithropanopeus harrisii (Gould, 1841) is listed as an non-native species in European waters. In Poland, it occurred in the 1950s at two sites at the Baltic Sea coast, the Dead Vistula River (DVR) and the Vistula Lagoon (VL). Almost 50 years later, two additional populations were identified in the Gulf of Gdańsk (GG) and its inner part, Puck Bay (PB). In the present study, we sequenced and analyzed part of the mitochondrial cytochrome oxidase subunit I gene of the four Polish populations of R. harrisii in order to assess their genetic diversity and connectivity. The analyzed sequences of a length of 989 base pairs revealed eight different haplotypes. The highest number of haplotypes (n=6) was observed in the population from GG, whereas the lowest (n=3) in VL. The most common haplotype was recorded in 37% of the analyzed individuals. Pairwise Φ ST values were mostly non-significant, with the exception of the comparison between DVR and VL (Φ ST = 0.267; P < 0.05) and between PB and VL (Φ ST = 0.194; P < 0.05), indicating a restricted gene flow or different sources of colonization.

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“…In Russia, Marin (2013) confirmed the occurrence of only H. takanoi from Sivuch ya Bay (near the border between Russia and North Korea) to Vostok Bay, and speculated that Sakhalin Island and other cold-water regions of the Sea of Japan could be inhabited by H. takanoi. The method presented here will also assist the species identification of non-indigenous H. penicillatus (Asian brush-clawed crab), which has been recorded since 1993 from Northeast Atlantic coasts (Europe, see Asakura & Watanabe 2005, Gothland et al 2014, Markert et al 2014; the crab specimens have not been confidently identified as either H. penicillatus or H. takanoi in most of the introduced areas (see Asakura & Watanabe 2005), though all 10 of the specimens collected on the German coast of the central Wadden Sea in 2008 were identified as H. takanoi by both molecular and mor- phological analyses (Markert et al 2014). Markert et al (2014) concluded that the morphological identification was not confidently feasible, but their qualitative data of spot sizes (large or small, Table 1 in Markert et al 2014) seem to indicate that the spot size on the ventral face of the cephalothorax and on the third maxillipeds could be a useful diagnostic character to distinguish H. penicillatus and H. takanoi, supporting the results of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Later, form II was described as a new species, H. takanoi, and form I was identified as H. penicillatus sensu stricto (Asakura & Watanabe 2005). Recently, sequence analysis of mitochondrial and nuclear genes confirmed that H. penicillatus and H. takanoi are distinct at the species level (Yama saki et al 2011, Markert et al 2014.…”

Section: Introductionmentioning

confidence: 99%

“…However, dark spots are often absent on the abdominal segments of H. penicillatus (Yamasaki et al 2011, Markert et al 2014), whereas they are sometimes present there in H. takanoi (Markert et al 2014), suggesting that the presence or absence of dark spots on the abdomen may not be a reliable diagnostic character. As for the dark spots on the ventral face of the cephalothorax, third maxillipeds and chelipeds, Sakai (2007) compared the number and the relative size (large or small) of these dark spots between H. penicillatus and H. takanoi and reported that it was difficult to distinguish the two species in this manner because the dark spots were random in number and shape.…”

Section: Introductionmentioning

confidence: 99%

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Usefulness of the size of dark spots on the body surface as a diagnostic character distinguishing two morphologically similar <i>Hemigrapsus</i> species (Decapoda: Brachyura: Varunidae)

Makino

Kan

Sato

et al. 2015

Plankton Benthos Res

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Abstract:The size of dark spots on the body surface was evaluated quantitatively as a diagnostic character to distinguish two species of varunid crabs of similar morphology: Hemigrapsus penicillatus and H. takanoi, common estuarine species in eastern Asia. From 37 Japanese localities, 171 H. penicillatus (106 males, 65 females) and 185 H. takanoi (113 males, 72 females) were collected and identified to species level by a molecular method. Spot size (the longest diameter of the largest dark spot) on the ventral face of the cephalothorax and on the outer faces of chelae were measured, with carapace width as an index of body size. Although the spot sizes at both loci increased with increases in carapace width, the spot sizes were significantly larger in H. penicillatus than H. takanoi. In both sexes, the ranges of variation in spot size partially overlapped between the species but the extent of such interspecific overlap was smaller for spot sizes on the ventral face of the cephalothorax in both sexes. Based on the spot size on the ventral face of the cephalothorax and carapace width, 92% and 82% of males and females were identified correctly into species via quadratic discriminant analyses. The spot size measured from the ventral face of the cephalothorax was therefore judged to be a useful diagnostic character to distinguish H. penicillatus and H. takanoi.

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Molecular identification and morphological characteristics of native and invasive Asian brush-clawed crabs (Crustacea: Brachyura) from Japanese and German coasts: Hemigrapsus penicillatus (De Haan, 1835) versus Hemigrapsus takanoi Asakura & Watanabe 2005

Cited by 27 publications

References 39 publications

Identification ofNorthSea molluscs withDNAbarcoding

Identification ofNorthSea molluscs withDNAbarcoding

Genetic diversity of the non-native crab Rhithropanopeus harrisii (Brachyura: Panopeidae) in the Polish coastal waters − an example of patchy genetic diversity at a small geographic scale

Usefulness of the size of dark spots on the body surface as a diagnostic character distinguishing two morphologically similar <i>Hemigrapsus</i> species (Decapoda: Brachyura: Varunidae)

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