Molecular identification and pathogenicity study of virulent Vibrio cholerae non O1/O139 serotype associated with mortality of farmed Labeo rohita (Hamilton, 1822), in India

Devi, Manoharmayum Shaya; Paria, Prasenjit; Kumar, Vikash; Parida, Pranaya Kumar; Maurye, Praveen; Behera, Bijay Kumar; Das, Basanta Kumar

doi:10.1016/j.aquaculture.2021.737529

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…Although V. cholerae is not referred to as a primary fish pathogen following Koch's postulates, it has been isolated from several freshwater and marine fish, which are considered a broad reservoir of V. cholerae strains that may cause infections in humans (Halpern and Izhaki, 2017 ). More recently, Devi et al ( 2022 ) reported on a non-O1, non-O139 V. cholerae serotype (EMM1) capable of inducing high mortality in the freshwater species Labeo rohita , suggesting that V. cholerae strains other than the typical human pathogens shall be considered relevant aquatic pathogens as well. Vibriosis caused by the abovementioned species is the most common and devastating bacterial disease in fish larviculture and aquaculture, being a public health and economical concern affecting marine fishes, crustaceans, and bivalves worldwide (Balebona et al, 1998a ; Sarjito et al, 2009 ; Ringø, 2020 ).…”

Section: Vibrio Species and Vibriosis In Aquaculturementioning

confidence: 99%

Vibriosis Outbreaks in Aquaculture: Addressing Environmental and Public Health Concerns and Preventive Therapies Using Gilthead Seabream Farming as a Model System

2022

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Bacterial and viral diseases in aquaculture result in severe production and economic losses. Among pathogenic bacteria, species belonging to the Vibrio genus are one of the most common and widespread disease-causing agents. Vibrio infections play a leading role in constraining the sustainable growth of the aquaculture sector worldwide and, consequently, are the target of manifold disease prevention strategies. During the early, larval stages of development, Vibrio species are a common cause of high mortality rates in reared fish and shellfish, circumstances under which the host organisms might be highly susceptible to disease preventive or treatment strategies such as vaccines and antibiotics use, respectively. Regardless of host developmental stage, Vibrio infections may occur suddenly and can lead to the loss of the entire population reared in a given aquaculture system. Furthermore, the frequency of Vibrio–associated diseases in humans is increasing globally and has been linked to anthropic activities, in particular human-driven climate change and intensive livestock production. In this context, here we cover the current knowledge of Vibrio infections in fish aquaculture, with a focus on the model species gilthead seabream (Sparus aurata), a highly valuable reared fish in the Mediterranean climatic zone. Molecular methods currently used for fast detection and identification of Vibrio pathogens and their antibiotic resistance profiles are addressed. Targeted therapeutic approaches are critically examined. They include vaccination, phage therapy and probiotics supplementation, which bear promise in supressing vibriosis in land-based fish rearing and in mitigating possible threats to human health and the environment. This literature review suggests that antibiotic resistance is increasing among Vibrio species, with the use of probiotics constituting a promising, sustainable approach to prevent Vibrio infections in aquaculture.

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Section: Vibrio Species and Vibriosis In Aquaculturementioning

confidence: 99%

Vibriosis Outbreaks in Aquaculture: Addressing Environmental and Public Health Concerns and Preventive Therapies Using Gilthead Seabream Farming as a Model System

2022

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“…The bacterial isolate was primarily distinguished by the Gram-staining technique [33]. The strain was then subjected to biochemical characterization following standard procedure, viz., lysine utilization, oxidase, lactose, trehalose, glucose, raffinose, melibiose, saccharose, adonitol, cellobiose, rhamnose, xylose, arabinose, malonate utilization, esculin hydrolysis, indole, Voges Proskauer's (VP), methyl red, H 2 S production, citrate utilization, phenylalanine utilization, nitrate reduction, urease and ONPG (β-galactosidase) by using the biochemical kit (KB003, HiMedia, Mumbai, India).…”

Section: Biochemical Characterizationmentioning

confidence: 99%

“…To perform the liquid phase assay, the bacterial cells were centrifuged for 5 min at 5000 rpm, and the resulting cell pellet was washed three times with PBS before being resuspended in PBS. The bacterial cell suspension and Sheep RBC were gently mixed in a 1:1 ratio, with a final 2% RBC concentration maintained [33]. After centrifuging the mixture for 30 min at 1200 rpm, it was incubated at 37 • C. Later, the hemolysis was observed spectrophotometrically at 540 nm [41] every 2 h until 18 h [40] on CLARIOSTAR R Plus (BMG Labtech, Ortenberg, Germany).…”

Section: Hemolytic Propertymentioning

confidence: 99%

“…The virulence genes like aerA (Aerolysin), act (Cytotoxic enterotoxin), ser (Serine protease), gcaT (Glycerophospholipid: cholesterol acyltransferase), Lip (Lipase), ast (Cytotonic enterotoxin), alt (Heat labile cytotonic enterotoxin), ahyB (Elastase), exu (Dnase), hlyA (Hemolysins) and ascV (Type III secretion system) were analyzed individually using gene-specific primers (Table 1). The conventional procedures for DNA extraction by the sarkosyl method, virulence gene amplification, gel electroporation, and gel image were taken according to the process described previously [33].…”

Section: Identification Of Virulence Genesmentioning

confidence: 99%

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Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

et al. 2023

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A case of severe mortality in farmed Labeo rohita was investigated to characterize the causative agent. We identified the bacterial strain as Aeromonas veronii isolated from the gut of infected L. rohita by biochemical assay, scanning electron microscopy and 16S rRNA gene sequence analysis. The in vivo challenge experiment showed that the LD50 of A. veronii was 2.2 × 104 CFU/fish. Virulence gene investigation revealed that the isolated A. veronii possesses Aerolysin, Cytotoxic enterotoxin, Serine protease, Dnase and Type III secretion system genes. The isolated strain was resistant to two antibiotics (ampicillin and dicloxacillin) while susceptible to 22 other antibiotics. The study further revealed that A. veronii induced both stresses along with non-specific and specific immune responses marked by elevated cortisol HSP70, HSP90 and IgM levels in the treated L. rohita fingerlings. Although the bacterial pathogen enhances the immune response, the negative effect on fish, including stress, and high mortality, create concern and a need for A. veronii management in L. rohita farms. The knowledge gained from this study would facilitate future research aimed at assessing the pathogenicity of A. veronii, with an emphasis on microbial disease management in other farmed fish species.

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“…are referred to as somatic antigen type-1/139 non-agglutinable strains (SANAS-1/139- Vc or non-serogoup agglutinating O1/O139 V. cholerae (NSA-O1/139 Vc ) (Chatterjee et al 2011 ; Sack et al 2003 ). The SANAS-1/139- Vc member does not possess cell surface polysaccharide and antigenic determinant for agglutinating somatic antgen type-1/139 as the name specifies, yet its members ( V. cholerae strains) (Shan et al 2022 ; Devi et al 2022 ; Malayil et al 2011 ) may be implicated in cholera outbreak case, wound, septicemia, gastro-enteritis and diarrhea. These Vibrio members have been in existence in the environment (water, plankton/sediment and fishes) since the discovery of the family Vibrionaceae in 1890 (Huq et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance

2022

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Somatic antigen agglutinable type-1/139 Vibrio cholerae (SAAT-1/139- Vc ) members or O1/O139 V. cholerae have been described by various investigators as pathogenic due to their increasing virulence potential and production of choleragen. Reported cholera outbreak cases around the world have been associated with these choleragenic V. cholerae with high case fatality affecting various human and animals. These virulent Vibrio members have shown genealogical and phylogenetic relationship with the avirulent somatic antigen non-agglutinable strains of 1/139 V. cholerae (SANAS-1/139- Vc ) or O1/O139 non-agglutinating V. cholerae (O1/O139-NAG- Vc ). Reports on implication of O1/O139-NAGVc members in most sporadic cholera/cholera-like cases of diarrhea, production of cholera toxin and transmission via consumption and/or contact with contaminated water/seafood are currently on the rise. Some reported sporadic cases of cholera outbreaks and observed change in nature has also been tracable to these non-agglutinable Vibrio members (O1/O139-NAGVc) yet there is a sustained paucity of research interest on the non-agglutinable V. cholerae members. The emergence of fulminating extraintestinal and systemic vibriosis is another aspect of SANAS-1/139- Vc implication which has received low attention in terms of research driven interest. This review addresses the need to appraise and continually expand research based studies on the somatic antigen non-serogroup agglutinable type-1/139 V. cholerae members which are currently prevalent in studies of water bodies, fruits/vegetables, foods and terrestrial environment. Our opinion is amassed from interest in integrated surveillance studies, management/control of cholera outbreaks as well as diarrhea and other disease-related cases both in the rural, suburban and urban metropolis.

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Molecular identification and pathogenicity study of virulent Vibrio cholerae non O1/O139 serotype associated with mortality of farmed Labeo rohita (Hamilton, 1822), in India

Cited by 12 publications

References 32 publications

Vibriosis Outbreaks in Aquaculture: Addressing Environmental and Public Health Concerns and Preventive Therapies Using Gilthead Seabream Farming as a Model System

Vibriosis Outbreaks in Aquaculture: Addressing Environmental and Public Health Concerns and Preventive Therapies Using Gilthead Seabream Farming as a Model System

Aeromonas veronii Is a Lethal Pathogen Isolated from Gut of Infected Labeo rohita: Molecular Insight to Understand the Bacterial Virulence and Its Induced Host Immunity

Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance

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