Molecular identification and phylogenetic analysis of GABA-producing lactic acid bacteria isolated from indigenous dadih of West Sumatera, Indonesia

Abstract

Background: Dadih (fermented buffalo milk) is a traditional Indonesian food originating from West Sumatra province. The fermentation process is carried out by lactic acid bacteria (LAB), which are naturally present in buffalo milk. Lactic acid bacteria have been reported as one of potential producers of γ-aminobutyric acid (GABA). GABA acts as a neurotransmitter inhibitor of the central nervous system. Methods: In this study, molecular identification and phylogenetic analysis of GABA producing LAB isolated from indigenous dadih of West Sumatera were determined. Identification of the GABA-producing LAB DS15 was based on conventional polymerase chain reaction. 16S rRNA gene sequence analysis was used to identify LAB DS15. Results: PCR of the 16S rRNA gene sequence of LAB DS15 gave an approximately 1400 bp amplicon. Phylogenetic analysis showed that LAB DS15 was Pediococcus acidilactici, with high similarity of 99% at 100% query coverage to Pediococcus acidilactici strain DSM 20284. Conclusions: It can be concluded that GABA producing LAB isolated from indigenous dadih was Pediococcus acidilactici.

Strain-level Identification of Beneficial Lactobacilli of Dairy Origin using 16S rRNA Sequencing: A Biotechnology Approach

“…brevisN1 showed 100% similarity at 100% query coverage for L. brevis (365799371) and was considered same species. 99% at 100% query coverage in 16S rRNA gene sequence analysis has been documented to having a high homology level which affirms the strain of the organism [24]. a phylogenetic tree has been described by [23] to produce a clear cut bootstrap value.…”

Bioprospecting of Fragrant Ginger (Zingiber aromaticum) Endophytic Bacteria from Enggano Island, Indonesia as Antimicrobial Compounds Producer

“…Genomic DNA was extracted by using Presto™ Mini gDNA Bacteria Kit (Genaid, GBB100, Taiwan) based on manufacture protocol. All isolates were amplified using 16S rRNA to investigate the bacterial species based on minor modifications from Anggraini et al, (2018). All amplicon products were then sent to Genetika Sains Indonesia in Jakarta/Indonesia for sequencing.…”

Characterization of Dadih: Traditional Fermented Buffalo Milk of Minangkabau

“…The LAB isolated from indigenous dadih from West Sumatra was reported as a potential producer of GABA, identified as Pediococcus acidilactici [19] and L. plantarum N5 [18]. The optimization of GABA production by P. acidilactici DS15 [74] and L. plantarum [18] were then investigated.…”

“…Dadih is known as a potential probiotic as it has a huge amount of LAB (typically 10 8 colony-forming units (cfu/g) [8,9], which potentially support the intestinal wall and has many benefits for life and health of the host [10]. These LAB of dadih were reported to have antimicrobial (antipathogenic) properties [10], hypocholesterolemic properties [11][12][13], antimutagenicity properties [14], antioxidant properties [15], immunomodulatory properties [16,17], anti-stress properties [18,19], and folate-producing ability [20].…”