Molecular Identification of Acidophilic Manganese (Mn)-Solubilizing Bacteria from Mining Effluents and Their Application in Mineral Beneficiation

Sanket, A. S.; Ghosh, Shreya; Sahoo, R.; Nayak, Sanghamitra; Das, Alok Prasad

doi:10.1080/01490451.2016.1141340

Cited by 61 publications

(24 citation statements)

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“…Finally, the isolation of acidophilic microorganisms on solid media is very difficult due to production of monosaccharides and oligosaccharides from gelling agents under extreme acidic conditions. In fact, acidophilic microbes are poorly growing on solid media , however, several attempts were made for acidophilic bacterial isolation by using plating techniques in recent years . The plating technique used in this study exhibited effective medium for the isolation of indigenous acidophilic bacteria on solid 9KFe +2 media used and improved by Khalid et al with little modification in solidifying agent using agarose instead of gelrite and isolated indigenous iron‐oxidizing bacteria.…”

Section: Discussionmentioning

confidence: 80%

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Sajjad

Zheng

et al. 2018

J Basic Microbiol

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The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous ironoxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod. The optimum growth pH and temperature for all isolates were 1.5 and 30°C, respectively, and showed extreme acidophilic nature. All the isolates showed obligate chemoautotrophic nature and used ferrous iron and pyrite as an energy source, however, isolates WG102 and WG103 were unable to use sulfur, while isolate WG101 could use elemental sulfur and reduced inorganic sodium thiosulfate as an energy source. The phylogenetic analyses based on 16S rRNA sequences revealed that the isolates WG101, WG102, and WG103 were homologous with Acidithiobacillus ferrooxidans strain AS2 (99%), Leptospirillum ferriphilum strain YSK (98%), and Leptospirillum ferrooxidans strain L15 (98%), respectively. These bacterial isolates showed efficient copper and zinc dissolution from the ore. The metals dissolution rate of At. ferrooxidans strain WG101 was 54.5 ± 4.33% (copper) and 49.6 ± 5% (zinc). The metals recovery rate of L.ferriphilum strain WG102 was 45.7 ± 3.5% (copper) and 40.5 ± 2.5% (zinc). The recovery rate of copper and zinc was 49.6 ± 4% and 46.5 ± 3% respectively in the case of L. ferrooxidans strain WG103. The findings of this study are consistent with the notion that the indigenous bacteria are more efficient in minerals dissolution. K E Y W O R D SAcidithiobacillus ferrooxidans, Baiyin Copper Mine, bioleaching, Leptospirillum ferriphilum, Leptospirillum ferrooxidansAbbreviations: AMD, acid mine drainage; EPS, exopolysaccharide; ORP, oxidation-reduction potential.

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Section: Discussionmentioning

confidence: 80%

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Sajjad

Zheng

et al. 2018

J Basic Microbiol

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“…Bacillus cereus can cause diarrhoeal and emetic syndrome with concentrations ranging from 10 3 -10 7 cells or spores, although the exact dose response has not yet been established as described by Santa María et al 37 . Bacillus cereus AMSB3 has also been reported as an Manganese solubilizer according to Sanket et al 38 , together with Bacillus nealsonii AMSB4, Enterobacter spp. AMSB1, and Staphylococcus hominis AMSB5.…”

Section: Scientific Reports |mentioning

confidence: 94%

Bacillus spp. characterization and his intervention as a possible non-traditional etiology of chronic renal insufficiency in Tierra Blanca, Veracruz, Mexico

Quiñones-Muñoz

Villares-Bueno

Ramírez

et al. 2020

Sci Rep

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Environmental, socioeconomic, educational, custom, occupation, and native pathogen microbiota factors have been identified as unique etiological factors by region for chronic renal insufficiency (CRI). In the region of Tierra Blanca, Veracruz, there is a significant incidence of CRI. The objective of this research was to identify the presence of the genus Bacillus spp. and its kinetic characterization for recognition as a possible non-traditional etiology of cRi in the region. the methodology included the isolation and morphological, biochemical, molecular and kinetic characterization of strains of the genus Bacillus spp. and an analysis of factors that indicate that their presence could affect the occupational health of the population, prompting cases of CRI. The presence of Bacillus cereus (pathogenic strain for humans) was established (biochemical identification, similarity 99%, by 16S rRNA gene) in sugarcane crops, mainly in the MEX-69-290 variety, with the higher growth rate and lower lag phase, compared to the other isolates. the strains are reported as a potential danger of direct infection and a risk factor for the indirect development of CRI, in the non-traditional cause modality, in the sugarcane fields. it is recommended that committed actions be undertaken to protect and promote the health of the population.Chronic kidney insufficiency (CRI) is defined as kidney damage or a glomerular filtration rate lower than 60 mL/ min per 1.73 m 2 for three months, greater urinary albumin excretion, or both 1 . 8-16% of the population worldwide is affected by CRI 2 . Hypertension, diabetes mellitus, which causes 31% of cases of CRI due to diabetic nephropathy according to Rajapurkar et al. 3 , urinary tract infections, obesity, and smoking, have been reported as important risk factors for the presence of microalbuminuria (an indicator of kidney injury, between 30 and 300 mg in 24 h) 4 . CRI is considered as a serious threat, as it is usually detected in an advanced state, it is irreversible, and besides increasing the possibility of death, CRI is costly to treat and consumes financial resources at an alarming rate.In addition to the underestimation of cases of CRI, it has been reported that its incidence and prevalence, along with other diverse pathologies, differ substantially across different countries and regions 2 , indicating that 1

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“…Multicopper oxidase family enzymes are now known to be involved coping mechanism that is inherently present in them (Ghosh, Mohanty, Nayak, Sukla, & Das, 2015;Mohanty et al, 2016b;Sanket et al, 2016). Negligible Mn recovery was observed in the sterile control flasks.…”

Section: Possible Mechanism For Variation In Protein Expressionmentioning

confidence: 99%

“…Almost half of the natural reserves of Mn consists of low-grade ores. Several studies have been reported on bioleaching of Mn using micro-organisms, but most of these are restricted to use a few well-known bacterial strains (Sanket, Ghosh, Sahoo, Nayak, & Das, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bioleaching of manganese from mining waste residues using Acinetobacter sp.

Ghosh

Das

2017

Geology, Ecology, and Landscapes

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The rapid decrease of natural resources and generation of huge amount of metallic wastes from mining industries has led to the focus of researchers to shift to alternative methods of waste benefaction and resource recycling. This study aims at the development of an eco friendly technique to recover Manganese (Mn) from mining waste residues using Acinetobacter sp. Bioleaching experiments were conducted in shake flasks at initial pH 6.5, 5% w/v inoculums and 2% pulp density at 30 °C with agitation speed 200 RPM and Acinetobacter sp. as inoculum. Mn recovery of 76% was recorded in 20 days. The analysis of the changes in cellular protein expression and conformation was carried out through sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and fourier transform infrared spectroscopy. The results reveal that bioleaching can alter protein expression and also result in conformational changes in protein structure. The present study sheds light on the greener alternative to recover and recycle manganese from wastes native bacteria. Exciting prospects for the utilization of mining wastes are in store in the near future; providing an economic and ecologically sound alternative to pyrometallurgical processes.

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Molecular Identification of Acidophilic Manganese (Mn)-Solubilizing Bacteria from Mining Effluents and Their Application in Mineral Beneficiation

Cited by 61 publications

References 24 publications

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Culture‐dependent hunt and characterization of iron‐oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction

Bacillus spp. characterization and his intervention as a possible non-traditional etiology of chronic renal insufficiency in Tierra Blanca, Veracruz, Mexico

Bioleaching of manganese from mining waste residues using Acinetobacter sp.

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