Molecular Identification of Insect Pests of Horticultural Crops

Mani, M.; Venkatesan, T.; Chethan, B. R.

doi:10.1007/978-981-19-0343-4_1

Cited by 2 publications

(2 citation statements)

References 92 publications

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“…DNA barcoding can discriminate various life stages of insects, i.e., egg, nymph, and adult. This technique also needs a small amount of sample and could determine new variations in the undescribed species (Mani et al, 2022). This study aimed to identify the Helopeltis sp.…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene

Pancaningtyas¹,

Sari²,

Rahayu³

2022

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Helopeltis sp. is one of the major pests of the cocoa plant. Helopeltis can decrease cocoa yield by 50 to 60%. Several species of Helopeltis spp. are still often misidentified due to similarity in morphology such as size and color. This study aimed to identify Helopeltis sp. through DNA barcoding using the mitochondrial cyto- chrome C oxidase subunit 1 (COI) gene. Analysis was carried out on 100 adults Helopeltis sp. from Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. Specimens were preserved in 96% ethyl alcohol at 4oC until molecular studies were carried out. PCR amplification was conducted through specific primer from COI gene. Sequences data analysis using MEGA 5.05 software was carried out. The study results using DNA barcoding showed that Helopeltis samples in Kaliwining cocoa plantations are Helopeltis bradyi with a percentage identity above 99% and E value of 0; this sequence as significantly matched. Mean length of the sequences was 679.5 base pairs. Phy- logenetic tree data also shows that the Helopeltis species analysed were also of the same descent, H. bradyi with the accession number KT189562.1. Helopeltis sp. specimens were clustered together. No taxonomic deviation was detected at the species level, indicating that most of the examined species of Helopeltis sp. could be authenticated by the barcode approach using the COI gene. In addi- tion, Helopeltis specimens were also aligned with H. antonii and H. theivora from the genebank to determine their genetic relationship.

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Section: Introductionmentioning

confidence: 99%

DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene

Pancaningtyas¹,

Sari²,

Rahayu³

2022

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“…Extensive research has shown that DNA barcoding can identify a wide range of animal species, including mammals, reptiles, birds, fishes, amphibians and crustaceans 12,28,30 . The classification and identification of various life forms, particularly insects have been a major challenge to the scientific community especially with the dwindling interest and funding for taxonomy 16 . DNA barcoding provides an alternative approach, allowing expedited examination of species richness of all animal lineages at comparatively low cost.…”

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confidence: 99%

Molecular Identification of the Diversity of Insects, Spiders, Lizards, Birds and Mammals of Tuensang District, Nagaland, India

Phom,

Somasundaram

2024

Biosci., Biotech. Res. Asia

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DNA barcoding is a method of species identification that revolutionized the way we study and understand biodiversity. With advancements in molecular genetics and DNA sequence archives, it has become possible to use short DNA sequences to identify species, even when the specimens are difficult to distinguish by traditional morphological methods. In this article, we explore the utility of DNA barcoding in the Tuensang ecosystem of Nagaland, India and evaluate its effectiveness for species identification, informing ongoing conservation of populations and species. A total of 62 species, which included insects, spiders, lizards, birds and mammals, were collected from Tuensang areas of northeast India and identified using DNA barcodes. DNA was extracted from muscle tissue and PCR was done with two pairs of primers targeting the mitochondrial COI gene. Sanger sequencing was employed and the obtained sequences were analysed to identify the species and reconstruct the evolutionary relationships amongst them. Our results provided molecular characterization of species from Tuensang areas of Nagaland for the first time.

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Molecular Identification of Insect Pests of Horticultural Crops

Cited by 2 publications

References 92 publications

DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene

DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene

Molecular Identification of the Diversity of Insects, Spiders, Lizards, Birds and Mammals of Tuensang District, Nagaland, India

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