Molecular Imprinted Polymers for Mass Sensitive Sensors: Comparation of Performance Toward Immuno-Sensing Strategies

Brimo, Nura; Serdaroğlu, Dilek Çökeliler

doi:10.1016/b978-0-12-822117-4.00013-7

Cited by 2 publications

(1 citation statement)

References 127 publications

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“…Recent studies involved the use of different polymers for MI. , In fact, some authors presented different MI products for different purposes, such as food applications, enzyme degradation, or amino acid-specific recognition. − In this sense, the main novelty of our work is the combined use of synthetic polymers with natural polymers to develop MI products with specific sites for biological recognition. Only a few studies have combined these two techniques since the use of proteins and other biomacromolecules still poses an important challenge …”

Section: Introductionmentioning

confidence: 99%

(Macro)Molecular Imprinting of Proteins on PCL Electrospun Scaffolds

Perez‐Puyana

Wieringa

Guerrero

et al. 2021

ACS Appl. Mater. Interfaces

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Biological recognition sites are very useful for biomedical purposes and, more specifically, for polymeric scaffolds. However, synthetic polymers are not capable of providing specific biological recognition sites. To solve this inconvenience, functionalization of biological moieties is typically performed, oftentimes via peptide binding. In this sense, the main task is capturing the biological complexity of a protein. This study proposes a possible alternative solution to this challenge. Our approach is based on the combination of molecular imprinting (MI) and electrospinning processes. We propose here an alternative MI approach with polymeric structures, instead of using cross-linkers and monomers as conventionally performed. Different PCL–protein scaffolds were produced via electrospinning before performing MI. Gelatin, collagen, and elastin were used as proteins. Results evidenced that the MI process conducted with PCL electrospun membranes was carried out with ionic interactions between the desired molecules and the recognition sites formed. In addition, it has been proved that MI was more efficient when using gelatin as a template. This approach opens a new stage in the development of recognition sites in scaffolds obtained with synthetic polymers and their application for biomedical purposes.

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Section: Introductionmentioning

confidence: 99%

(Macro)Molecular Imprinting of Proteins on PCL Electrospun Scaffolds

Perez‐Puyana

Wieringa

Guerrero

et al. 2021

ACS Appl. Mater. Interfaces

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Detection of Immunochemical Reactions Using Piezoquartz Immunosensor. Regeneration of the Electrode Bio-layer (Review)

Gogina,

Smirnova,

Stanishevskii

et al. 2024

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. One of the most promising types of immunosensors is quartz crystal microbalance immunosensors (QCM immunosensors). Single-use biosensors are financially demanding, thus rendering the regeneration of the biosensor surface a pertinent issue for QCM immunosensors. Regeneration plays a pivotal role in sustaining the functionality of the sensor and enabling its reusability. In this article, "immunosensor" and "immunobiosensor" are interchangeable terms and are used to denote the same type of biosensors operating based on immunochemical interactions between antigens and antibodies.Text. This review discusses the features, operational principles, and applications of QCM immunosensors. Particular attention is directed toward the challenge of regenerating the biosensor surface as a key aspect ensuring their effective operation and the potential for multiple uses. Various regeneration methods and their advantages are examined. The reactivation of the biosensing layer on the QCM electrode secures its stability and functionality over extended periods, which is especially valuable in clinical and scientific research. The possibility of reusing the biosensor reduces material costs and waste production, aligning with ecological and economic concerns. Furthermore, the ability to analyze different analytes on the same surface fosters versatility in multiparametric investigations. It is essential to emphasize that the removal of residual analytes and the biosensor's regeneration process enhance reliability, selectivity, heightened sensitivity, and the potential for reproducible measurements.Conclusion. An analysis of scientific literature underscores the pivotal role of biosensor regeneration in maintaining functionality and reusability. The strength of the antigen-antibody interaction determines the conditions, which must be tailored individually for each antigen-antibody pair. The review thoroughly explores three primary approaches to the regeneration of piezoelectric transducers, including the use of a chemical method, oxygen plasma-based techniques, and the application of Piranha solution.

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Molecular Imprinted Polymers for Mass Sensitive Sensors: Comparation of Performance Toward Immuno-Sensing Strategies

Cited by 2 publications

References 127 publications

(Macro)Molecular Imprinting of Proteins on PCL Electrospun Scaffolds

(Macro)Molecular Imprinting of Proteins on PCL Electrospun Scaffolds

Detection of Immunochemical Reactions Using Piezoquartz Immunosensor. Regeneration of the Electrode Bio-layer (Review)

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