Molecular interactions in the retinal basement membrane system: A proteomic approach

Balasubramani, Manimalha; Schreiber, Emanuel M.; Candiello, Joseph; Balasubramani, Goundappa K.; Kurtz, Justin; Halfter, Willi

doi:10.1016/j.matbio.2010.04.002

Cited by 55 publications

(52 citation statements)

References 61 publications

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“…2). Most likely this is because the laminin β2 chain is the predominant β subunit in the ILM (Balasubramani et al, 2010), and other laminin β subunits fail to compensate for its loss in the Lamb2 and Lamb2:c3 mutants.…”

Section: Discussionmentioning

confidence: 99%

“…The laminin α1, α5, β2 and γ1 chains are the predominant laminin subunits present in the ILM (Balasubramani et al, 2010); thus, the dominant laminins in the ILM are likely to be 121 and 521. The treatment of Lamb2 and Lamb2:c3-null retinal cultures with EHSlaminin (containing mostly laminin 111) or laminin 521 provided physiologically relevant ligands for integrin expression and activation regulating migration and spatial positioning.…”

Section: Discussionmentioning

confidence: 99%

“…We next tested whether the addition of laminin-521, a laminin composed of the predominant laminin chains found in the native ILM (Balasubramani et al, 2010) would restore astrocyte behavior. In untreated controls, astrocytes were clumped together and did not have their normal morphology (supplementary material Fig.…”

Section: Research Articlementioning

confidence: 99%

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Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina

et al. 2013

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SUMMARYPathologies of retinal blood vessels are among the major causes of blindness worldwide. A key cell type that regulates retinal vascular development is the astrocyte. Generated extrinsically to the retina, astrocytes migrate into the retina through the optic nerve head. Even though there is a strong correlation between astrocyte distribution and retinal vascular development, the factors that guide astrocytes into the retina remain unclear. In this study, we show that astrocytes migrate within a laminin-containing basement membrane -the inner limiting membrane. Genetic deletion of the laminin β2 and γ3 chains affects astrocyte migration and spatial distribution. We show that laminins act as haptotactic factors in vitro in an isoform-specific manner, inducing astrocyte migration and promoting astrocyte differentiation. The addition of exogenous laminins to laminin-null retinal explants rescues astrocyte migration and spatial patterning. Furthermore, we show that the loss of laminins reduces β1 integrin expression in astrocytes. Culturing lamininnull retinal astrocytes on laminin substrates restores focal localization of β1 integrin. Finally, we show that laminins containing β2 and γ3 chains regulate subsequent retinal blood vessel growth and maintain vascular integrity. These in vivo and in vitro studies demonstrate clearly that laminins containing β2 and γ3 chains are indispensable for migration and spatial organization of astrocytes and that they play a crucial role during retinal angiogenesis in vivo. RESEARCH ARTICLELaminins regulate angiogenesis treatment with exogenous laminins rescues astrocyte migration and spatial patterning phenotype ex vivo. Together, these data support the hypothesis that β2-and γ3-containing laminins are important cues in retinal vascular development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina

et al. 2013

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“…The epithelial/mesenchymal interface of most tissues is marked by the presence of a basement membrane. These thin, uniform insoluble sheets of highly specialized extracellular matrix proteins are found in all multicellular organisms [66], but vary in composition in a tissue-specific manner [67]. Basement membranes serve as substrates for epithelial cells, endothelial cells and myotubes [68].…”

Section: Ilm Materials Properties In Chromovitrectomymentioning

confidence: 99%

Material Properties of the Internal Limiting Membrane and Their Significance in Chromovitrectomy

Henrich

Monnier²,

Loparić

et al. 2013

Ophthalmologica

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Intraoperative visualization of the internal limiting membrane (ILM), the choice of a point of vantage for lifting an initial flap, the precision with which the ILM is grasped, adhesion between the forceps and the ILM, thickness, stiffness and elasticity of the ILM as well as monitoring of the completeness of ILM removal are all important factors for safety and efficacy of a chromovitrectomy intervention. The understanding of the underlying physical features of the ILM, such as contrast behavior and bioanatomical and biomechanical properties represent, thus, useful prerequisites for successful macular surgery. New analytical tools, such as atomic force microscopy and chromaticity analysis, allow new insights into ILM material characteristics, permitting a systematic approach to refinement of surgical technique.

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“…Proteomic characterization of mammary gland extracts identified basement membrane components able to support epithelial morphogenesis [23]. Novel nidogen and heparin interactions, identified by proteomic analysis of retinal basement membranes, indicated new roles for heparan sulfate proteoglycans in stabilizing the vitreous-basement membrane interface [33]. Comprehensive analysis of decellularized vascular ECM revealed a cohort of novel components, including sclerostin, a glycoprotein more commonly associated with regulating bone mineralization [25].…”

Section: Novel Approaches To Target the Ecm Proteomementioning

confidence: 99%

The extracellular matrix: an underexplored but important proteome

Wilson¹

2010

Expert Review of Proteomics

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Molecular interactions in the retinal basement membrane system: A proteomic approach

Cited by 55 publications

References 61 publications

Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina

Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina

Material Properties of the Internal Limiting Membrane and Their Significance in Chromovitrectomy

The extracellular matrix: an underexplored but important proteome

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