Molecular lipidomics of exosomes released by PC-3 prostate cancer cells

Llorente, Alicia; Skotland, Tore; Sylvänne, Tuulia; Kauhanen, Dimple; Róg, Tomasz; Orłowski, Adam; Vattulainen, Ilpo; Ekroos, Kim; Sandvig, Kirsten

doi:10.1016/j.bbalip.2013.04.011

Cited by 616 publications

(630 citation statements)

References 49 publications

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“…6). These data revealed that, as expected from our previous study (39), exosomes released from control PC-3 cells are enriched in CHOL, SM, and glycosphingolipids and contained lower levels of PL than the parent cells (Fig. 6a, left and middle panels), and there is an ϳ6-fold enrichment (6.2 Ϯ 0.3; S.D., n ϭ 3) of lipids/mg of protein in exosomes compared with the parent cells.…”

Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etsupporting

confidence: 80%

“…Exosomes released by PC-3 cells are enriched in CHOL, SM, PS, and glycosphingolipids, although they contain less PC than the parent cells (39). Because the addition of the ether lipid precursor HG modifies the cellular lipidome (Figs.…”

Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etmentioning

confidence: 99%

“…2-4), we decided to investigate whether the lipid composition of exosomes released from PC-3 cells was changed after HG treatment. Exosomes released during a period of 17-19 h from control, HG-, and DP-treated cells were isolated from the cell culture medium by ultracentrifugation as described previously (39). As shown in Fig.…”

Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etmentioning

confidence: 99%

“…Exosome Isolation-Exosomes were isolated from the conditioned media of control and treated cells as described elsewhere (39). Briefly, the conditioned media were centrifuged at 300 ϫ g for 10 min, 1,000 ϫ g for 10 min, and 10,000 ϫ g for 30 min, discarding the pellet at each step.…”

Section: Role Of Ether Lipids In Exosome Releasementioning

confidence: 99%

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The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Phuyal

Skotland

Hessvik

et al. 2015

Journal of Biological Chemistry

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119

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Background: Lipids are emerging as important constituents of the molecular machinery involved in the formation and release of exosomes. Results: The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes. Conclusion: Ether lipids are important modulators of exosome release. Significance: By knowing the machinery involved in exosome release, it would be possible to modulate this process.

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Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etsupporting

confidence: 80%

Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etmentioning

confidence: 99%

Section: Ether Lipid Precursor Hg Increases the Exosomal Levels Of Etmentioning

confidence: 99%

Section: Role Of Ether Lipids In Exosome Releasementioning

confidence: 99%

See 2 more Smart Citations

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Phuyal

Skotland

Hessvik

et al. 2015

Journal of Biological Chemistry

Self Cite

119

View full text Add to dashboard Cite

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“…Lipidomic analysis showed an accumulation of cholesterol, sphingomyelin, glycerophospholipids and phosphatidylserine (PS) in EVs [34]. Certain membrane-associated proteins, for example many tetraspanins (e.g.…”

Section: Biogenesis Cargo Loading and Compositionmentioning

confidence: 99%

Therapeutic and diagnostic applications of extracellular vesicles

Stremersch

Smedt

Raemdonck

2016

Journal of Controlled Release

159

103

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During the past two decades, extracellular vesicles (EVs) have been identified as important mediators of intercellular communication, enabling the functional transfer of bioactive molecules from one cell to another. Consequently, it is becoming increasingly clear that these vesicles are involved in many (patho)physiological processes, providing opportunities for therapeutic applications. Moreover, it is known that the molecular composition of EVs reflects the physiological status of the producing cell and tissue, rationalizing their exploitation as biomarkers in various diseases. In this review the composition, biogenesis and diversity of EVs is discussed in a therapeutic and diagnostic context. We describe emerging therapeutic applications, including the use of EVs as drug delivery vehicles and as cell-free vaccines, and reflect on future challenges for clinical translation. Finally, we discuss the use of EVs as a biomarker source and highlight recent studies and clinical successes.

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The lipid‐binding D4 domain of perfringolysin O facilitates the active loading of exogenous cargo into extracellular vesicles

Opadele,

Nishioka,

et al. 2024

FEBS Letters

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Whereas extracellular vesicles (EVs) have been engineered for cargo loading, innovative strategies for it can still be developed. Here, we describe domain 4 (D4), a cholesterol‐binding domain derived from perfringolysin O, as a viable candidate for EV cargo loading. D4 and its mutants localized to the plasma membrane and the membranes of different vesicular structures in the cytoplasm, and facilitate the transport of proteins of interest (POIs) into EVs. D4‐EVs were internalized by recipient cells analogous to EVs engineered with CD9. Intracellular cargo discharge from D4‐EVs was successfully detected with the assistance of vesicular stomatitis virus glycoprotein. This study presents a novel strategy for recruiting POIs into EVs via a lipid‐binding domain that ensures content release in recipient cells.

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Molecular lipidomics of exosomes released by PC-3 prostate cancer cells

Cited by 616 publications

References 49 publications

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 Cells

Therapeutic and diagnostic applications of extracellular vesicles

The lipid‐binding D4 domain of perfringolysin O facilitates the active loading of exogenous cargo into extracellular vesicles

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