Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

Graner, Andreas; Streng, S.; Kellermann, A.; Schiemann, A.; Bauer, Eva; Waugh, Robbie; Pellio, Bettina; Ordon, Frank

doi:10.1007/s001220051070

Cited by 77 publications

(49 citation statements)

References 23 publications

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“…It was partially and acceptably resistant to BaYMV-III. Chromosomal locations of rym1 (Miyazaki et al 2001) and rym5 (Graner et al 1995, 1999, Miyazaki et al 2001 on RFLP linkage map have been identified. A new (7HS) QTL for BaYMV resistance was also identified in Mokusekko 3 (Miyazaki et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Interaction between Barley Yellow Mosaic Disease-resistance Genes rym1 and rym5, in the Response to BaYMV Strains

et al. 2004

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A Chinese landrace of barley, Mokusekko 3, is completely resistant to all the present strains of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), and has at least two resistance genes, rym1 and rym5. However, rym1 has not been used for BaYMV-resistance breeding of barley in Japan so far. In our previous study, rym1 was completely resistant to BaYMV-I, -II, BaMMV-Ka1 and -Na1, and was acceptably resistant to BaYMV-III. Complete resistance of Mokusekko 3 was estimated to be due to the interaction between rym1 and rym5. To estimate pyramiding effects of these genes using DNA markers, we investigated the resistance reaction of lines with or without these genes to BaYMV-I and -III. All the pyramiding lines showed complete resistance to BaYMV-I and -III. This study anticipated the resistance to BaYMV-III of Mokusekko 3 by the multiple effect of the rym1 and rym5 genes.

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Section: Introductionmentioning

confidence: 99%

Interaction between Barley Yellow Mosaic Disease-resistance Genes rym1 and rym5, in the Response to BaYMV Strains

et al. 2004

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“…Although at least seven independent loci conferring resistance to BYMV have been identified in barley to date (Ordon et al 2005), European breeding has relied heavily on rym4 and rym5, both of which originate from single germplasm accessions. The Dalmatian landrace Ragusa is the source of rym4 (Huth 1985), while rym5 derives from the Chinese landrace Mokusekko 3 (Konishi et al 1997;Graner et al 1999;Friedt et al 2000).The cloning of Hv-eIF4E Wicker et al 2005) provides a focus for an LD analysis. Taking into consideration that breeding for resistance in Europe did not start until the 1980s, and that the Hv-eIF4E locus has been subjected to a high selection pressure over a short timescale, this case study reveals much of how genome structure and dynamics can be shaped by plant breeding.…”

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confidence: 99%

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confidence: 99%

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Stracke

Presterl²,

Stein

et al. 2007

Genetics

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We present a detailed analysis of linkage disequilibrium (LD) in the physical and genetic context of the barley gene Hv-eIF4E, which confers resistance to the barley yellow mosaic virus (BYMV) complex. Eightythree SNPs distributed over 132 kb of Hv-eIF4E and six additional fragments genetically mapped to its flanking region were used to derive haplotypes from 131 accessions. Three haplogroups were recognized, discriminating between the alleles rym4 and rym5, which each encode for a spectrum of resistance to BYMV. With increasing map distance, haplotypes of susceptible genotypes displayed diverse patterns driven mainly by recombination, whereas haplotype diversity within the subgroups of resistant genotypes was limited. We conclude that the breakdown of LD within 1 cM of the resistance gene was generated mainly by susceptible genotypes. Despite the LD decay, a significant association between haplotype and resistance to BYMV was detected up to a distance of 5.5 cM from the resistance gene. The LD pattern and the haplotype structure of the target chromosomal region are the result of interplay between low recombination and recent breeding history.T HE ever-increasing availability of nucleotide sequence, and the concomitant improvement that this brings to our understanding of the organization of complex crop plant genomes, has created the opportunity to identify trait-related genes and to analyze their allelic diversity. The association between phenotype and genotype in diverse populations represents a powerful approach, but the applicability and design of such analyses depend critically on the extent and pattern of linkage disequilibrium (LD) present in the study population. Cultivated barley (Hordeum vulgare ssp. vulgare) has many of the hallmarks known to be associated with a high level of LD. Its effective recombination rate is dramatically reduced by its predominantly inbreeding habit, with an estimated outcrossing rate of 5% in winter barley and ,0.5% in spring barley (Giles et al. 1974;Doll 1987;Abdel-Ghani et al. 2005). Domestication and intensive selection have introduced major bottlenecks in genetic variation, and these are thought to be largely responsible for the perceived narrowness of the modern gene pool (Badr et al. 2000;Russell et al. 2000;Matus and Hayes 2002). LD in modern spring barley, as estimated from a whole-genome survey, extends over distances of at least 10 cM, indicative of an extensive conservation of the genetic identity of barley chromosomes (Kraakman et al. 2004). However, estimates of genomewide LD conceal localized variation, which, as has been shown for a number of species, can be substantial and independent of the mating system (Gupta et al. 2005). In the self-pollinating species Arabidopsis thaliana LD varies from ,10 kb in global (Tian et al. 2002) to 50-250 kb in local populations (Nordborg et al. , 2005Aranzana et al. 2005). Within a given set of maize (an outbreeding species) accessions, the extent of LD has been documented to vary widely around different genes and chr...

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“…On chromosome 2HS, a QTL for resistance to net blotch of adult plants has been described (Pecchioni et al 1996;Steffenson et al 1996). Other qualitative genes for disease resistance including Ryd2 for resistance to barley yellow dwarf virus (Collins et al 1996), Rhy, Rh, and Rrs for scald resistance Graner et al 2000;Genger et al 2003, this issue), and rym 4, rym5, and rym11 for virus resistance to strains of BaMMV and BaYMV (Graner and Bauer 1993;Bauer et al 1997;Graner et al 1999), also mapped close to Pt.a/Pt.1 gene for net blotch resistance on the proximal region of 3HL. On chromosome 4H, a large region of the genetic map of Sloop/Halcyon covering ~50 cM exhibited significant association with NFNB resistance (Fig.…”

Section: Discussionmentioning

confidence: 99%

Mapping of genomic regions associated with net form of netblotch resistance in barley

Raman¹,

Platz²,

Chalmers³

et al. 2003

Aust. J. Agric. Res.

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Abstract. Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9-17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population. In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F 2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon.A R 0 3 0 2 6 M a p p i n g n e t b l o t c h r e s i s t a n c e H . R a m a n e t a l .

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Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex

Cited by 77 publications

References 23 publications

Interaction between Barley Yellow Mosaic Disease-resistance Genes rym1 and rym5, in the Response to BaYMV Strains

Interaction between Barley Yellow Mosaic Disease-resistance Genes rym1 and rym5, in the Response to BaYMV Strains

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Mapping of genomic regions associated with net form of netblotch resistance in barley

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