Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat

Dholakia, Bhushan B.; Rajwade, Ashwini V.; Hosmani, Prashant S.; Khan, Rashad Rasool; Chavan, Sachin G.; Reddy, Devarapalli Mohan; Lagu, M. D.; Bansal, Urmil; Saini, R. G.; Gupta, Vidya S.

doi:10.1007/s11032-012-9813-9

Cited by 19 publications

(7 citation statements)

References 28 publications

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“…This would suggest that Capo has a race specific response at this interval. Several leaf rust genes are assigned to 2DS of which Lr15 , Lr22 (alleles Lr22a, Lr22b) and Lr39 have been mapped to telomeric regions of chromosome 2DS (Dholakia et al 2013 ; Hiebert et al 2007 ; Raupp et al 2001 ), whereas Lr2 (alleles Lr2a , Lr 2 b , Lr 2 c ) is more proximal (McIntosh et al 1995 ). Capo does not carry Lr2a , Lr2c and Lr15 (Winzeler et al 2000 ; Park et al 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals co-location of three QTL conferring resistance to both rust pathogens

et al. 2014

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Key messageWe detected several, most likely novel QTL for adult plant resistance to rusts. Notably three QTL improved resistance to leaf rust and stripe rust simultaneously indicating broad spectrum resistance QTL.AbstractThe rusts of wheat (Puccinia spp.) are destructive fungal wheat diseases. The deployment of resistant cultivars plays a central role in integrated rust disease management. Durability of resistance would be preferred, but is difficult to analyse. The Austrian winter wheat cultivar Capo was released in the 1989 and grown on a large acreage during more than two decades and maintained a good level of quantitative leaf rust and stripe rust resistance. Two bi-parental mapping populations: Capo × Arina and Capo × Furore were tested in multiple environments for severity of leaf rust and stripe rust at the adult plant stage in replicated field experiments. Quantitative trait loci associated with leaf rust and stripe rust severity were mapped using DArT and SSR markers. Five QTL were detected in multiple environments associated with resistance to leaf rust designated as QLr.ifa-2AL, QLr.ifa-2BL, QLr.ifa-2BS, QLr.ifa-3BS, and QLr.ifa-5BL, and five for resistance to stripe rust QYr.ifa-2AL, QYr.ifa-2BL, QYr.ifa-3AS, QYr.ifa-3BS, and QYr.ifa-5A. For all QTL apart from two (QYr.ifa-3AS, QLr.ifa-5BL) Capo contributed the resistance improving allele. The leaf rust and stripe rust resistance QTL on 2AL, 2BL and 3BS mapped to the same chromosome positions, indicating either closely linked genes or pleiotropic gene action. These three multiple disease resistance QTL (QLr.ifa-2AL/QYr.ifa-2AL, QLr.ifa.2BL/QYr.ifa-2BL, QLr.ifa-3BS/QYr.ifa.3BS) potentially contribute novel resistance sources for stripe rust and leaf rust. The long-lasting resistance of Capo apparently rests upon a combination of several genes. The described germplasm, QTL and markers are applicable for simultaneous resistance improvement against leaf rust and stripe rust.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2357-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals co-location of three QTL conferring resistance to both rust pathogens

et al. 2014

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“…Diagnostic markers have also been developed for the above three Yr genes; similar markers can also be developed for other non-allelic Lr and Sr genes which may accelerate the haplotype analysis and expedite stacking of different genes through MAS. (iii) MQTL2D.1 is co-localized with a seedling LR resistance gene (Lr15) (Dholakia et al 2013), two adult plant YR resistance genes (Yr16, Yr54) and an all-stage YR resistance gene (Yr55) (Rani et al 2019). (iv) MQTL3A.1 overlap with Lr63, Yr76; Lr63 conditions low to intermediate infection types to most P. triticina isolates, however in combinations with other effective seedling or AP resistance genes, it can be used to develop wheat cultivars with highly effective LR resistance.…”

Section: Discussionmentioning

confidence: 99%

Meta-QTLs for multiple disease resistance involving three rusts in common wheat (Triticum aestivum L.)

Pal

Jan

Saini

et al. 2022

Preprint

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Rust diseases in wheat are a major threat to global food security, therefore, the development of multiple disease-resistant cultivars (resistant to all three rusts) is a major goal in all wheat breeding programs worldwide. In the present study, meta-QTLs and candidate genes for multiple disease resistance (MDR) involving all the three rusts were identified using 152 individual QTL mapping studies for resistance to leaf rust (LR), stem rust (SR), and yellow rust (YR). From these 152 studies, a total of 1,146 QTLs for resistance to the three rusts were retrieved, which included 368 QTLs for LR, 291 QTLs for SR, and 487 QTLs for YR. Of these 1,146 QTLs, only 718 QTLs could be projected onto the consensus map saturated with 234,619 markers. Meta-analysis of the projected QTLs resulted in the identification of 86 MQTLs, which included 71 MDR-MQTLs. Ten of these MDR-MQTLs were referred to as the ‘Breeders’ MQTLs’. Seventy-eight of the 86 MQTLs could also be anchored to the physical map of the wheat genome and 54 MQTLs were validated by marker-trait associations identified during earlier genome-wide association studies. Twenty MQTLs (including 17 MDR-MQTLs) identified in the present study were co-localized with 42 known R genes. In silico expression analysis allowed identification of several differentially expressed candidate genes encoding proteins carrying different domains including the following: NBS-LRR, WRKY domains, F-box domains, sugar transporters, transferases, etc. The introgression of these MDR loci into high-yielding cultivars should prove useful for developing high yielding cultivars with resistance to all three rusts.

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“…The molecular markers for majority of approximately 69 Lr genes (Huerta Espino et al 2011) were reported (Purnhauser et al 2000) while further were extended by other ones mostly at 2D (Dholakia et al 2013) or 7D chromosomes (Huseinova et al 2013) where various Lr genes were located (Mc Intosh 1998). Diagnostic value of molecular markers regarding vertical resistance was discussed by Sefing et al (2011).…”

Section: Introductionmentioning

confidence: 99%

Tolerance of wheat varieties seedlings by glyphosate accelerated degradation

Jerkovic¹,

Prijic²,

Jevtić³

et al. 2014

Ratar i povrt

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Summary:Lengths of seedlings aboveground parts of nine wheat varieties grown in greenhouse were reduced by 4-20%. They were tested during April at around 20 o C after twice daily treatments with glyphosate based herbicide (0.5 ml in 1l water solution, 0.5 dl on 0.5 m 2 ). When results of permanent artificially and daily lighted trials were compared, four groups of varieties were recognized same as was when near isogenic lines containing different leaf rust resistance genes (Lr NILs) were focused in winter. Growth of variety Pobeda decreased more when seedlings were not permanent lighted which confirmed the common herbicide effect. Tolerance of other varieties was again explained by enzymatic degradation of glyphosate and chlorophyll inhibition by consequential phosphorus acids. Varieties Simonida, Tavita and Rapsodia were similar by reaction to glyphosate as Lr 1 and Lr 21 NILs. NS 40S contained Lr 3a with increased hydrolytic stability compared to Lr 24 but able to cleave external single phosphorus for the difference of Lr 19 or Lr 29. Their final effect was similar to previous stated of Lr 15 NIL during the winter. Varieties Gora and Metka had gene Lr 19, Enigma Lr 29 while Ilina Lr 24. Focusing herbicide residua transfer in seed probability, accounting optimal time for recovering ability of chlorophyll and temporary higher temperatures necessary for Lr genes even parasite free activity the varieties Gora, Metka and Enigma appeared to be possible solution for glyphosate apply near wheat heading in semiarid regions.

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Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat

Cited by 19 publications

References 28 publications

Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals co-location of three QTL conferring resistance to both rust pathogens

Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals co-location of three QTL conferring resistance to both rust pathogens

Meta-QTLs for multiple disease resistance involving three rusts in common wheat (Triticum aestivum L.)

Tolerance of wheat varieties seedlings by glyphosate accelerated degradation

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