Molecular Mechanisms behind Persistent Presence of Parvovirus B19 in Human Dilated Myocardium

Bironaitė, Daiva; Kažukauskienė, Ieva; Bogomolovas, Julius; Daunoravičius, Dainius; Jakubauskas, Artūras; Vitkus, Dalius; Žurauskas, Edvardas; Ručinskas, Kęstutis; Labeit, Siegfried; Grabauskienė, Virginija

doi:10.1007/5584_2021_702

Cited by 3 publications

(1 citation statement)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When examining B19V viral loads, no significant differences were reported between patients with heart disease and controls ( Schenk et al, 2009 ), suggesting that the amount of virus in the heart tissue cannot be used to predict heart disease. Recently, Bironaitė et al reported that chronic persistence of B19V in the myocardium was not correlated with lymphocyte infiltration; however, the heart tissue samples positive for the viral genome exhibited mitochondrial impairment ( Bironaitė et al, 2022 ), suggesting that another mechanism may be behind B19V-induced heart injury. The inflammatory processes that occur in the aftermath of viral infection can also induce tissue damage.…”

Section: Parvovirus B19mentioning

confidence: 99%

Persistent viral infections and their role in heart disease

2022

View full text Add to dashboard Cite

Viral infections are the culprit of many diseases, including inflammation of the heart muscle, known as myocarditis. Acute myocarditis cases have been described in scientific literature, and viruses, such as parvovirus B19, coxsackievirus B3, or more recently, SARS-CoV-2, were the direct cause of cardiac inflammation. If not treated, myocarditis could progress to dilated cardiomyopathy, which permanently impairs the heart and limits a person’s lifespan. Accumulated evidence suggests that certain viruses may persist in cardiac tissue after the initial infection, which could open up the door to reactivation under favorable conditions. Whether this chronic infection contributes to, or initiates, cardiac damage over time, remains a pressing issue in the field of virus-induced heart pathology, and it is directly tied to patients’ treatment. Previously, large case studies found that a few viruses: parvovirus B19, coxsackievirus, adenovirus, human herpesvirus 6, cytomegalovirus and Epstein–Barr virus, are most commonly found in human endomyocardial biopsy samples derived from patients experiencing cardiac inflammation, or dilated cardiomyopathy. SARS-CoV-2 infection has also been shown to have cardiovascular consequences. This review examines the role of viral persistence in cardiac inflammation and heart disease, and discusses its implications for patients’ outcomes.

show abstract

Section: Parvovirus B19mentioning

confidence: 99%

Persistent viral infections and their role in heart disease

2022

View full text Add to dashboard Cite

show abstract

Detection of Parvovirus B19 genome in human heart tissue samples

Kloc,

Campbell,

Espinoza

2023

BMC Res Notes

View full text Add to dashboard Cite

Objective Identifying viral genomes in human heart tissues is critical for disease diagnosis and assessment of cardiovascular damage. Human heart tissue samples obtained during a biopsy procedure are routinely used to test for the presence of viruses, as guided by clinical manifestations and prognosis. Furthermore, heart tissue samples obtained post-mortem or during a cardiac transplant procedure serve as a valuable research tool, as they allow for an in-depth assessment of cardiac pathology that can aid in our understanding of molecular pathways associated with disease. Because viral nucleic acid constitutes only a small portion of each sample’s genetic material, appropriate methods are necessary for positive viral genome identification. Results Snap-frozen heart tissue samples obtained either post-mortem or during a cardiac transplant procedure were used to develop conditions for detection of Parvovirus B19. Briefly, total DNA was isolated from the heart tissue under varying conditions. A PCR-based assay with Parvovirus B19 specific primers was implemented to detect the presence of the viral genome, followed by Sanger Sequencing. The mechanical disruption of the heart tissue, as well as the cardiac tissue processing methods, had a significant effect on the DNA quality and the ability to detect the Parvovirus B19 genome.

show abstract

Analysis of Parvovirus B19 persistence and reactivation in human heart layers

Badrinath,

Gardere,

Palermo

et al. 2024

Front. Virol.

View full text Add to dashboard Cite

Heart disease is the leading cause of death worldwide. Myocarditis, or inflammation of the cardiac muscle, is estimated to cause up to 1.5 million cases annually, with viral infection being the most common disease culprit. Past studies have shown that Parvovirus B19 is routinely detected in endomyocardial biopsies. This virus has been linked to acute heart inflammation, which can cause cardiac muscle damage. However, because Parvovirus B19 can be found in the heart tissues in the absence of disease symptoms, it is unclear if the long-term presence of the virus contributes to, or initiates, heart disease. Here, we utilized a PCR-based detection assay to assess the presence of the B19V genome and its mRNA intermediates in human heart tissues. The analysis was carried out in three heart layers derived from one individual: epicardium, endocardium and myocardium. We showed the Parvovirus B19 genome presence variability in different heart layers. Similarly, viral transcriptional activity, assessed by the mRNA presence, was detected only in a few of the analyzed samples. Our results suggest that localized sites of Parvovirus B19 infection may exist within individual heart layers, which may have implication for the cardiac muscle inflammation.

show abstract

Molecular Mechanisms behind Persistent Presence of Parvovirus B19 in Human Dilated Myocardium

Cited by 3 publications

References 80 publications

Persistent viral infections and their role in heart disease

Persistent viral infections and their role in heart disease

Detection of Parvovirus B19 genome in human heart tissue samples

Analysis of Parvovirus B19 persistence and reactivation in human heart layers

Contact Info

Product

Resources

About