Molecular Mechanisms Underlying Micronutrient Utilization in Marine Diatoms

Coale, Tyler H.; Bertrand, Erin M.; Lampe, Robert H.; Allen, Andrew E.

doi:10.1007/978-3-030-92499-7_20

Cited by 6 publications

(6 citation statements)

References 289 publications

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“…Genome‐enabled exploration of Phaeodactylum tricorutum has also revealed important features of diatom nutrient metabolism (Coale et al., 2022; Kroth & Matsuda, 2022; Smith & Allen, 2022). Examples include the discovery of a functional urea cycle involved in the metabolism optimization under nitrogen limitation (Allen et al., 2011), or the role of the nitrate reductase in controlling the balance between nitrate transport and assimilation, and the distribution of carbon flux (Levitan et al., 2015; McCarthy et al., 2017).…”

Section: Insight Into the Biology Of Diatomsmentioning

confidence: 99%

“…It is also worth mentioning that the first transcriptomic studies performed in P. tricornutum cells under iron‐limited conditions led to the identification of novel regulators of the iron response, such as the iron starvation‐induced proteins (ISIPs; Allen et al., 2008). Following later functional studies (Coale et al., 2022), ISIPs are now widely used to predict the response to nutrient availability in environmental studies (Nef et al., 2022).…”

Section: Insight Into the Biology Of Diatomsmentioning

confidence: 99%

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Phaeodactylum tricornutum: An established model species for diatom molecular research and an emerging chassis for algal synthetic biology

Russo,

Rogato,

Jaubert

et al. 2023

Journal of Phycology

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Diatoms are prominent and highly diverse microalgae in aquatic environments. Compared with other diatom species, Phaeodactylum tricornutum is an “atypical diatom” displaying three different morphotypes and lacking the usual silica shell. Despite being of limited ecological relevance, its ease of growth in the laboratory and well‐known physiology, alongside the steady increase in genome‐enabled information coupled with effective tools for manipulating gene expression, have meant it has gained increased recognition as a powerful experimental model for molecular research on diatoms. We here present a brief overview of how over the last 25 years P. tricornutum has contributed to the unveiling of fundamental aspects of diatom biology, while also emerging as a new tool for algal process engineering and synthetic biology.

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Section: Insight Into the Biology Of Diatomsmentioning

confidence: 99%

Section: Insight Into the Biology Of Diatomsmentioning

confidence: 99%

Phaeodactylum tricornutum: An established model species for diatom molecular research and an emerging chassis for algal synthetic biology

Russo,

Rogato,

Jaubert

et al. 2023

Journal of Phycology

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“…Additional strategies include lower respiration rates, the use of proteorhodopsins, Fe-free proteins which may allow the production of additional ATP, and the replacement of the Fe-containing photosynthetic proteins, i.e., cytochrome c6 and ferredoxin, with the copper protein plastocyanin or the nonmetal protein flavodoxin, respectively, under Fe limitation. Fe-saving strategies have been studied mainly in diatoms and are discussed by Strzepek et al [79] and Coale et al [80].…”

Section: Lightmentioning

confidence: 99%

Southern Ocean Iron Limitation of Primary Production between Past Knowledge and Future Projections

Bazzani

Lauritano

Saggiomo

2023

JMSE

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Primary production in the Southern Ocean highly depends on phytoplankton and has been reported to be limited by the availability of the micronutrient iron. The aim of this review is to summarize the past and current knowledge on iron limitation in the Southern Ocean, and specifically how it affects primary producers, thus influencing the whole Southern Ocean community structure, carbon cycling, and large-scale ocean biogeochemistry. In this region, extensive variability exists between different areas regarding iron availability, but also between seasons. Moreover, co-limitations with other abiotic environmental factors exist, further complicating the assessment of the role of iron as limiting factor for phytoplankton productivity. Currently, climate change is altering the Southern Ocean environment. How these changes will affect resident phytoplankton is still not clear, possibly modifying the iron supply mechanisms. Existing projections point towards a possible partial relief of iron stress on phytoplankton, but the interactions between different environmental changes, and the cascade effects they will have, are still poorly understood, and some aspects understudied. Here we try to synthetize the available predictions and uncertainties concerning this topic.

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“…ZIP and CDF-family transporters can transport multiple divalent metal cations 79 ; therefore, if localized to the cell surface, they may serve as low affinity Fe(II) permeases to take advantage of potentially increased Fe(II) 25,83 . Direct Fe(II) uptake has been demonstrated in eukaryotic microalgae including diatoms supporting this role 84 .…”

Section: Differential Expression Of Transcripts and Proteins Among Co...mentioning

confidence: 99%

“…Other differentially expressed genes indicate an adoption of strategies that reduce cellular iron demand. Due to its versatile redox chemistry, iron plays an essential role in photosynthesis-related proteins in diatoms, and reductions in photosynthetic proteins can be related to iron stress 83 . In centric diatoms, higher CO 2 reduced protein abundances of the photosynthetic proteins cytochrome b6 (petB) at Experiment 1 as well as apocytochrome f (petA) and photosystem II D1 (psbA) at Experiment 3 (Fig.…”

Section: Differential Expression Of Transcripts and Proteins Among Co...mentioning

confidence: 99%

Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton

Lampe,

Coale,

Forsch

et al. 2023

Nat Commun

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Coastal upwelling regions are among the most productive marine ecosystems but may be threatened by amplified ocean acidification. Increased acidification is hypothesized to reduce iron bioavailability for phytoplankton thereby expanding iron limitation and impacting primary production. Here we show from community to molecular levels that phytoplankton in an upwelling region respond to short-term acidification exposure with iron uptake pathways and strategies that reduce cellular iron demand. A combined physiological and multi-omics approach was applied to trace metal clean incubations that introduced 1200 ppm CO2 for up to four days. Although variable, molecular-level responses indicate a prioritization of iron uptake pathways that are less hindered by acidification and reductions in iron utilization. Growth, nutrient uptake, and community compositions remained largely unaffected suggesting that these mechanisms may confer short-term resistance to acidification; however, we speculate that cellular iron demand is only temporarily satisfied, and longer-term acidification exposure without increased iron inputs may result in increased iron stress.

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Molecular Mechanisms Underlying Micronutrient Utilization in Marine Diatoms

Cited by 6 publications

References 289 publications

Phaeodactylum tricornutum: An established model species for diatom molecular research and an emerging chassis for algal synthetic biology

Phaeodactylum tricornutum: An established model species for diatom molecular research and an emerging chassis for algal synthetic biology

Southern Ocean Iron Limitation of Primary Production between Past Knowledge and Future Projections

Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton

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