Molecular mechanisms underlying postoperative peritoneal tumor dissemination may differ between a laparotomy and carbon dioxide pneumoperitoneum: a syngeneic mouse model with controlled respiratory support

Matsuzaki, Sachiko; Bourdel, Nicolas; Darcha, Claude; Déchelotte, P.; Bazin, Jean-Étienne; Pouly, Jean-Luc; Mage, G.; Canis, Michel

doi:10.1007/s00464-008-0041-7

Cited by 20 publications

(39 citation statements)

References 35 publications

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“…This line readily forms tumors that spread in the peritoneal cavity, similar to stages III and IV, in immunocompetent mice [9,10,12]. They were maintained in Dulbecco's modified eagle medium (DMEM) (Invitrogen), supplemented with 4% fetal calf serum (Invitrogen), 100 UI/mL penicillin, 100 lg/mL streptomycin, 5 lg/mL insulin, 5 lg/mL transferrin, and 5 lg/mL sodium selenite (ITS, Invitrogen).…”

Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

“…Anesthesia and videoendoscopy-assisted endotracheal intubation [7][8][9][10] All mice were anaesthetized in a container with 3-5% vaporized isoflurane with air. Each spontaneously breathing, anaesthetized animal was placed in the supine position on a surgical warming table.…”

Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

“…Homeothermia [7][8][9][10] After the induction of anaesthesia; all procedures were performed on a thermostatically regulated, feedbackcontrolled heating pad (Homeothermic Blanket Control Unit, Harvard Apparatus GmbH). After intubation, a temperature probe was inserted into the rectum and homeothermia was maintained during the surgical procedure.…”

Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

“…However, a serious limitation of many of the laparoscopic experiments performed in rodent models is the lack of attention paid to confounding factors, including whether or not respiratory support was used, and what insufflation pressures were used in the peritoneum [7,8]. Therefore, we developed a syngenic mouse model of postoperative peritoneal dissemination of ovarian cancer cells with controlled respiratory support (CRS) [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…In this model, we recently investigated the impact of surgical peritoneal environment on postoperative peritoneal dissemination [10]. The ovarian cancer cell line was inoculated just prior to surgery to mimic the spillage of tumor cells during surgery.…”

Section: Introductionmentioning

confidence: 99%

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Impact of surgical peritoneal environment on postoperative tumor growth and dissemination in a preimplanted tumor model

et al. 2008

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Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

Section: Inoculation Of Mouse Ovarian Cancer Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impact of surgical peritoneal environment on postoperative tumor growth and dissemination in a preimplanted tumor model

et al. 2008

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Experimental study of delivery of humidified-warm carbon dioxide during open abdominal surgery

Carpinteri

Sampurno

Malaterre

et al. 2018

Journal of British Surgery

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BackgroundThe aim of this study was to monitor the effect of humidified‐warm carbon dioxide (HWCO2) delivered into the open abdomen of mice, simulating laparotomy.MethodsMice were anaesthetized, ventilated and subjected to an abdominal incision followed by wound retraction. In the experimental group, a diffuser device was used to deliver HWCO2; the control group was exposed to passive air flow. In each group of mice, surgical damage was produced on one side of the peritoneal wall. Vital signs and core temperature were monitored throughout the 1‐h procedure. The peritoneum was closed and mice were allowed to recover for 24 h or 10 days. Tumour cells were delivered into half of the mice in each cohort. Tissue was then examined using scanning electron microscopy and immunohistochemistry.ResultsPassive air flow generated ultrastructural damage including mesothelial cell bulging/retraction and loss of microvilli, as assessed at 24 h. Evidence of surgical damage was still measurable on day 10. HWCO2 maintained normothermia, whereas open surgery alone led to hypothermia. The degree of tissue damage was significantly reduced by HWCO2 compared with that in controls. Peritoneal expression of hypoxia inducible factor 1α and vascular endothelial growth factor A was lowered by HWCO2. These effects were also evident at the surgical damage sites, where protection from tissue trauma extended to 10 days. HWCO2 did not reduce tumorigenesis in surgically damaged sites compared with passive air flow.ConclusionHWCO2 diffusion into the abdomen in the context of open surgery afforded tissue protection and accelerated tissue repair in mice, while preserving normothermia. Surgical relevanceDamage to the peritoneum always occurs during open abdominal surgery, by exposure to desiccating air and by mechanical trauma/damage owing to the surgical intervention. Previous experimental studies showed that humidified‐warm carbon dioxide (HWCO2) reduced peritoneal damage during laparoscopic insufflation. Additionally, this intervention decreased experimental peritoneal carcinomatosis compared with the use of conventional dry‐cold carbon dioxide.In the present experimental study, the simple delivery of HWCO2 into the open abdomen reduced the amount of cellular damage and inflammation, and accelerated tissue repair. Sites of surgical intervention serve as ideal locations for cancer cell adhesion and subsequent tumour formation, but this was not changed measurably by the delivery of HWCO2.

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Carbon dioxide pneumoperitoneum, intraperitoneal pressure, and peritoneal tissue hypoxia: a mouse study with controlled respiratory support

et al. 2010

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Molecular mechanisms underlying postoperative peritoneal tumor dissemination may differ between a laparotomy and carbon dioxide pneumoperitoneum: a syngeneic mouse model with controlled respiratory support

Cited by 20 publications

References 35 publications

Impact of surgical peritoneal environment on postoperative tumor growth and dissemination in a preimplanted tumor model

Impact of surgical peritoneal environment on postoperative tumor growth and dissemination in a preimplanted tumor model

Experimental study of delivery of humidified-warm carbon dioxide during open abdominal surgery

Carbon dioxide pneumoperitoneum, intraperitoneal pressure, and peritoneal tissue hypoxia: a mouse study with controlled respiratory support

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