Molecular Methods for Identification and Quantification of Foodborne Pathogens

Zhang, Min; Wu, Jiajia; Shi, Zhaoai; Cao, Aocheng; Fang, Wensheng; Yan, Deyue; Wang, Qiuxia; Li, Yuan

doi:10.3390/molecules27238262

Cited by 12 publications

(11 citation statements)

References 39 publications

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“…As PCR can only detect a single DNA sequence at a time, numerous PCR techniques are created and have been developed to detect the multiple pathogens present in a sample of food that is contaminated. Many of these techniques include multiplex PCR, nested PCR and reverse transcription PCR, as well as quantitative PCR to detect genetic information [124]. As multiplex PCR and quantitative PCR have become increasingly popular tools for detecting and diagnosing foodborne pathogens, our review aims to provide an overview of both techniques.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…A number of benefits are associated with multiplex PCR, including its ability to rapidly identify multiple species of harmful microorganisms, the high level of sensitivity and high specificity for the target organism [124][125][126]. By using this method, it is possible to quickly detect and magnify a wide range of bacteria in a single response, and multiple bacteria can be viewed at the same time.…”

Section: Multiplex Pcrmentioning

confidence: 99%

“…The multiplex PCR method was also successfully applied to identify Listeria monocytogenes in vegetables [128]. Using a multiplex PCR technique coupled with a membrane chip, Zhang et al [124] developed a method that simultaneously detected nine types of foodborne pathogens. A number of other methods have also been created, such as the FilmArrayTM Gastrointestinal Panel (BioFire Diagnostics ® , Salt Lake City, UT, USA) and the Gastrointestinal Pathogen Panel (GPP) created by Applied BioCode ® (Santa Fe Springs, CA, USA) to detect the 22 and 17 most commonly identified foodborne pathogens.…”

Section: Multiplex Pcrmentioning

confidence: 99%

“…It can be argued that due to these factors, the quantitative PCR technique has become one of the most commonly used methods for identifying and classifying foodborne pathogens for its accuracy and effectiveness. As a result of the rapid development of TaqManTM and LightCycleTM probes, quantitative PCR procedures are becoming more frequent [124]. It has previously been demonstrated that quantitative PCR has been used to identify three subtypes of the stx1 and seven subtypes of the stx2 associated with Shigella toxins [133].…”

Section: Quantitative Pcrmentioning

confidence: 99%

See 3 more Smart Citations

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

et al. 2023

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Pathogens found in food are believed to be the leading cause of foodborne illnesses; and they are considered a serious problem with global ramifications. During the last few decades, a lot of attention has been paid to determining the microorganisms that cause foodborne illnesses and developing new methods to identify them. Foodborne pathogen identification technologies have evolved rapidly over the last few decades, with the newer technologies focusing on immunoassays, genome-wide approaches, biosensors, and mass spectrometry as the primary methods of identification. Bacteriophages (phages), probiotics and prebiotics were known to have the ability to combat bacterial diseases since the turn of the 20th century. A primary focus of phage use was the development of medical therapies; however, its use quickly expanded to other applications in biotechnology and industry. A similar argument can be made with regards to the food safety industry, as diseases directly endanger the health of customers. Recently, a lot of attention has been paid to bacteriophages, probiotics and prebiotics most likely due to the exhaustion of traditional antibiotics. Reviewing a variety of current quick identification techniques is the purpose of this study. Using these techniques, we are able to quickly identify foodborne pathogenic bacteria, which forms the basis for future research advances. A review of recent studies on the use of phages, probiotics and prebiotics as a means of combating significant foodborne diseases is also presented. Furthermore, we discussed the advantages of using phages as well as the challenges they face, especially given their prevalent application in food safety.

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Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Section: Multiplex Pcrmentioning

confidence: 99%

Section: Multiplex Pcrmentioning

confidence: 99%

Section: Quantitative Pcrmentioning

confidence: 99%

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An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

et al. 2023

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“…In this study, the primer pairs used did not display the loss of amplification efficiency according to the optimization results. It is important to optimize the annealing condition to avoid any non-specific amplification and the low amplification efficiency scenario due to the interaction of two primer pairs in the multiplex PCR reaction [32,33]. Moreover, this PCR-LFD approach has been previously employed by other studies for a similar purpose.…”

Section: Discussionmentioning

confidence: 99%

Multiplex PCR-Lateral Flow Dipstick Method for Detection of Thermostable Direct Hemolysin (TDH) Producing V. parahaemolyticus

et al. 2023

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Vibrio parahaemolyticus is usually found in seafood and causes acute gastroenteritis in humans. Therefore, a detection method of pathogenic V. parahaemolyticus is necessary. Multiplex PCR combined with lateral flow dipstick (LFD) assay was developed to detect pathogenic V. parahaemolyticus. Biotin-, FAM-, and Dig-conjugated primers targeting thermolabile hemolysin (TLH) and thermostable direct hemolysin (TDH) genes were used for multiplex PCR amplification. The condition of the method was optimized and evaluated by agarose gel electrophoresis and universal lateral flow dipstick. The specificity assay was evaluated using strains belonging to seven foodborne pathogen species. The sensitivity of the method was also evaluated using DNA in the concentration range of 0.39–100 ng/reaction. The artificial spiking experiment was performed using 10 g of shrimp samples with an enrichment time of 0, 4, and 8 h with 101, 102, and 103 CFU of V. parahaemolyticus. The developed multiplex PCR-LFD assay showed no non-specific amplification with a limit of the detection of 0.78 ng DNA/reaction visualized by agarose gel electrophoresis and 0.39 ng DNA with LFD assay. The artificial spiking experiment demonstrated that this method could detect pathogenic V. parahaemolyticus at 10 CFU/10 g shrimp samples following a 4 h of enrichment. Multiplex PCR-LFD assay was therefore established for detecting pathogenic V. parahaemolyticus with high sensitivity and specificity and might be a useful tool to develop a detection kit used in the food safety sector.

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Detection of Foodborne Pathogens Through Volatile Organic Compounds Sensing via Metal Oxide Gas Sensors

Lawaniya,

Awasthi,

Menezes

et al. 2024

Advanced Sensor Research

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Foodborne pathogens are a crucial diagnostic target for the food, beverage, and healthcare sectors due to their ubiquity and the potential damage they may do to the public's well‐being, food safety, and the economy. Over the past few decades, there has been an increased focus on developing highly precise and trusted biosensors in an effort to eliminate the discrepancy between reporting demands and currently used traditional detection approaches. Metal oxide semiconductor (MOS)‐based gas sensors have rapidly advanced in recent years, becoming a dominating technology for developing devices in food‐quality management, biomedical research, and diagnostics. This review systematically explores recent advancements in gas sensing technologies utilizing metal oxide‐based sensors for the detection of foodborne pathogens through the analysis of volatile organic compounds (VOCs). The comprehensive discussion encompasses insights into various foodborne pathogens, their implications for human health, diverse metal oxide characteristics, strategies for enhancing their sensing capabilities, and the distinctive features of VOCs. Furthermore, a thorough examination of the utilization of different metal oxides in VOC sensing is provided, addressing both existing challenges and potential future developments. In summary, employing gas sensing techniques for foodborne pathogen detection holds substantial commercial promise compared to alternative bio‐sensing approaches.

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Molecular Methods for Identification and Quantification of Foodborne Pathogens

Cited by 12 publications

References 39 publications

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

Multiplex PCR-Lateral Flow Dipstick Method for Detection of Thermostable Direct Hemolysin (TDH) Producing V. parahaemolyticus

Detection of Foodborne Pathogens Through Volatile Organic Compounds Sensing via Metal Oxide Gas Sensors

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