Molecular Methods for the Detection and Characterization of Food‐Borne Pathogens

Rusul, Gulam; Chuah, Li‐Oon

doi:10.1002/9781118864463.ch29

Cited by 4 publications

(2 citation statements)

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“…The study analyzed the WGS data of S. enterica isolates from raw milk, cheese, and humans and identified the serotypes, subtypes, and antimicrobial resistance profiles of the isolates. This study also traced the sources and transmission routes of S. enterica in raw milk and cheese production [19][20][21].…”

Section: Some Application Examples Of the Molecular Methods For Ident...mentioning

confidence: 99%

Accurate Identification of Salmonella enterica in Calves

Kabir,

Khan,

Ur Rahman

2024

Salmonella - Current Trends and Perspectives in Detection and Control

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Salmonella enterica is a bacterium that can cause serious infections in humans and animals, especially cattle. The identification and differentiation of S. enterica serotypes in cattle is important for epidemiological surveillance, disease prevention and control, and public health protection. However, the current methods and techniques for S. enterica detection have various challenges and limitations, such as low sensitivity and specificity, high cost and complexity, and the need for viable and pure bacterial cultures. Therefore, there is a need for further research and development of novel and improved methods and techniques that can overcome these challenges and provide reliable and accurate information on S. enterica serotypes in cattle. Such information can help to improve the understanding of the epidemiology, pathogenesis, and transmission of S. enterica in cattle, as well as to design and implement effective strategies for its prevention and control. This chapter reviews the current methods and techniques for S. enterica detection, such as culture-based methods, biochemical methods, molecular methods, phage-based methods, and biosensor methods, and discusses their advantages and disadvantages, as well as their future trends and perspectives.

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Section: Some Application Examples Of the Molecular Methods For Ident...mentioning

confidence: 99%

Accurate Identification of Salmonella enterica in Calves

Kabir,

Khan,

Ur Rahman

2024

Salmonella - Current Trends and Perspectives in Detection and Control

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“…These antibodies can also cross-react with other E. coli serotypes and other members of the Enterobacteriaceae family [9]. In order to reduce the analysis time and improve the rapid, sensitive, and specific detection of pathogens, alternative techniques like molecular methods have been applied for detection from various sample types [10].…”

Section: Introductionmentioning

confidence: 99%

Contrast of Real-Time Fluorescent PCR Methods for Detection of Escherichia coli O157:H7 and of Introducing an Internal Amplification Control

2019

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Various constituents in food specimens can inhibit the PCR assay and lead to false-negative results. An internal amplification control was employed to monitor the presence of false-negative results in PCR amplification. In this study, the objectives were to compare the real-time PCR-based method by introducing a competitive internal amplification control (IAC) for the detection of Escherichia O157:H7 with respect to the specificity of the primers and probes, analytical sensitivity, and detection limits of contamination-simulated drinking water. Additionally, we optimized the real-time fluorescent PCR detection system for E. coli O157:H7. The specificity of primers and probes designed for the rfbE gene was evaluated using four kinds of bacterial strains, including E. coli O157:H7, Staphylococcus aureus, Salmonella and Listeria monocytogenes strains. The real time PCR assay unambiguously distinguished the E. coli O157:H7 strains after 16 cycles. Simultaneously, the lowest detection limit for E. coli O157:H7 in water samples introducing the IAC was 104 CFU/mL. The analytical sensitivity in water samples had no influence on the detection limit compared with that of pure cultures. The inclusion of an internal amplification control in the real-time PCR assay presented a positive IAC amplification signal in artificially simulated water samples. These results indicated that real-time fluorescent PCR combined with the IAC possessed good characteristics of stability, sensitivity, and specificity. Consequently, the adjusted methods have the potential to support the fast and sensitive detection of E. coli O157:H7, enabling accurate quantification and preventing false negative results in E. coli O157:H7 contaminated samples.

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