Molecular microbiology techniques

Zhu, Xiangyang; Al-Moniee, Mohammed A.

doi:10.1016/b978-0-08-101105-8.00022-x

Cited by 3 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Especially in the water, oil and gas industries, it is necessary to consider the great microbial diversity in the given environment, which cultivation techniques usually significantly skew. To prevent economic losses by microbial corrosion or acidification of oil, it is necessary to monitor the state and presence of different groups of microorganisms in, for example, tanks and pipes [113]. The total number of microorganisms per unit of volume or area can be determined by the DAPI method.…”

Section: Dapi Fluorescent Dyementioning

confidence: 99%

“…From these n bers, the percentage of SRB or even directly targeted species can be calculated [114 Using electron scanning microscopy, the present microbial population's appearance distribution can be further examined [115]. Molecular methods using PCR can then d mine the exact species or their corrosive activity in the sample [113].…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

See 1 more Smart Citation

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Kushkevych

Hýžová

Vítězová

et al. 2021

IJMS

View full text Add to dashboard Cite

This paper is devoted to microscopic methods for the identification of sulfate-reducing bacteria (SRB). In this context, it describes various habitats, morphology and techniques used for the detection and identification of this very heterogeneous group of anaerobic microorganisms. SRB are present in almost every habitat on Earth, including freshwater and marine water, soils, sediments or animals. In the oil, water and gas industries, they can cause considerable economic losses due to their hydrogen sulfide production; in periodontal lesions and the colon of humans, they can cause health complications. Although the role of these bacteria in inflammatory bowel diseases is not entirely known yet, their presence is increased in patients and produced hydrogen sulfide has a cytotoxic effect. For these reasons, methods for the detection of these microorganisms were described. Apart from selected molecular techniques, including metagenomics, fluorescence microscopy was one of the applied methods. Especially fluorescence in situ hybridization (FISH) in various modifications was described. This method enables visual identification of SRB, determining their abundance and spatial distribution in environmental biofilms and gut samples.

show abstract

Section: Dapi Fluorescent Dyementioning

confidence: 99%

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Kushkevych

Hýžová

Vítězová

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

Unveiling the complexities of microbiologically induced corrosion: mechanisms, detection techniques, and mitigation strategies

Ahmed,

Mahmoud,

Mohamed

2024

Front. Environ. Sci. Eng.

View full text Add to dashboard Cite

Preserving Microbial Community Integrity in Oilfield Produced Water

Rachel

Gieg

2020

Front. Microbiol.

View full text Add to dashboard Cite

Determining a representative microbial signature from any given location is dependent on robust sample collection and handling. Different sampling locations and hence sample properties can vary widely; for example, soil would be collected and handled differently compared to liquid samples. In the event that sample material has a low concentration of biomass, large quantities need to be collected for microbial community analysis. This is certainly the case when investigating the microbiology of oilfield systems, wherein produced water (PW) is one of the most common sources for microbial sampling. As the detrimental effects of microbial metabolism within these industrial milieus are becoming increasingly well-established, the characterization of microbial community composition using molecular biological analyses is becoming more commonplace for accurate monitoring. As this field continues to develop, the importance for standardized operating protocols cannot be understated, so that industry can make the most informed operational decisions possible. Accurately identifying oilfield microbial communities is paramount, as improper preservation and storage following sample collection is known to lead to erroneous microbial identifications. Preserving oilfield PW can be challenging, as many locations are remote, requiring lengthy periods of time before samples can be processed and analyzed. While previous studies have characterized the effects of various preservatives on concentrated, filtered, or purified microbial samples, to the best of our knowledge, no such study has been undertaken on low biomass liquid samples. To this end, we investigated the effectiveness of nine different preservation conditions on PW collected from the same sampling location within a heavy-oil producing field, and monitored how the microbial community changed over the period of a month. Our results reveal that the choice of preservative drastically affects microbial community, and should be selected with careful consideration before sampling occurs.

show abstract

Molecular microbiology techniques

Cited by 3 publications

References 55 publications

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Unveiling the complexities of microbiologically induced corrosion: mechanisms, detection techniques, and mitigation strategies

Preserving Microbial Community Integrity in Oilfield Produced Water

Contact Info

Product

Resources

About