Molecular modelling and biological evaluation of phyto-molecules as potential activators of gluconolactone oxidase (GULO)

Sivadas, Sneha; Mohanty, Amaresh Kumar; Rajesh, Saranga; Muthuvel, Suresh Kumar; Vasanthi, Hannah R.

doi:10.1080/07391102.2023.2187227

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…DHA is transported to the blood through GLUT. 99 The brain, muscles, adrenal glands, and liver absorb vitamin C via SVCT2, making the tens of mM compared with the tens of μM in the blood. Thus, vitamin C-decorated NDDSs can transport drugs to various organs via SVCT-mediated oral absorption and organ targeting, and can be applied to treat diseases in the brain, liver, and so on.…”

Section: Future Directions Beyond Gi Transporter/receptor-mediated Nd...mentioning

confidence: 99%

Beyond nanoparticle-based oral drug delivery: transporter-mediated absorption and disease targeting

Cho,

Huh,

Cho

et al. 2024

Biomater. Sci.

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Section: Future Directions Beyond Gi Transporter/receptor-mediated Nd...mentioning

confidence: 99%

Beyond nanoparticle-based oral drug delivery: transporter-mediated absorption and disease targeting

Cho,

Huh,

Cho

et al. 2024

Biomater. Sci.

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“…For example, vegetables are rich in vitamin C [4]. The human body needs a sufficient amount of vitamin C for normal activities, but the human body cannot synthesize it and can only obtain it through external sources [5]. A lack of vitamin C can lead to fatigue, ulcerations, and other symptoms [6].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen and Phosphorus Co-Doped Carbon Dots for the Growth Promotion of Water Spinach

Yang

She

et al. 2023

Symmetry

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Carbon dots have received much attention due to their unique physicochemical properties and diverse applications in bioimaging, optoelectronic devices, catalysis, and agriculture. Here, in this work, we report a simple hydrothermal synthesis of nitrogen and phosphorus−doped carbon dots (N, P−CDs). The optical and physical properties of the synthesized N, P−CDs are analyzed using systematical spectroscopy and electrical characterization. The synthesized N, P−CDs show strong photoluminescence at 626 nm and demonstrate high stability under UV light and other conditions. Moreover, we incorporate the synthesized N, P−CDs into water spinach by root spraying and leaf spraying. It is found that N, P−CDs could effectively promote the growth of water spinach by accelerating the photosynthetic rate, and increasing the content of total phenols, anthocyanins, and flavonoids in water spinach.

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L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis

Gad,

Sirko

2024

CIMB

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L-ascorbic acid (AsA, vitamin C) plays a vital role in preventing various diseases, particularly scurvy. AsA is known for its antioxidant properties, which help protect against reactive oxygen species generated from metabolic activities; however, at high doses, it may exhibit pro-oxidative effects. The final step in AsA biosynthesis is catalyzed by L-gulono-γ-lactone oxidase (GULO). This enzyme is present in many organisms, but some animals, including humans, guinea pigs, bats, and other primates, are unable to synthesize AsA due to the absence of a functional GULO gene. The GULO enzyme belongs to the family of aldonolactone oxidoreductases (AlORs) and contains two conserved domains, an N-terminal FAD-binding region and a C-terminal HWXK motif capable of binding the flavin cofactor. In this review, we explore AsA production, the biosynthetic pathways of AsA, and the localization of GULO-like enzymes in both animal and plant cells. Additionally, we compare the amino acid sequences of AlORs across different species and summarize the findings related to their enzymatic activity. Interestingly, a recombinant C-terminal rat GULO (the cytoplasmic domain of the rat GULO expressed in Escherichia coli) demonstrated enzymatic activity. This suggests that the binding of the flavin cofactor to the HWXK motif at the C-terminus is sufficient for the formation of the enzyme’s active site. Another enzyme, GULLO7 from Arabidopsis thaliana, also lacks the N-terminal FAD-binding domain and is strongly expressed in mature pollen, although its activity has not been specifically measured.

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Molecular modelling and biological evaluation of phyto-molecules as potential activators of gluconolactone oxidase (GULO)

Cited by 3 publications

References 25 publications

Beyond nanoparticle-based oral drug delivery: transporter-mediated absorption and disease targeting

Beyond nanoparticle-based oral drug delivery: transporter-mediated absorption and disease targeting

Nitrogen and Phosphorus Co-Doped Carbon Dots for the Growth Promotion of Water Spinach

L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis

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