Molecular, Morphological, and Pathogenicity Characterization of Sphaceloma Ampelinum Isolates From Thailand

Tharapreuksapong, Akkawat; Poolsawat, O.; Jenweerawat, Sujin; Wongkaew, Sopone; Thipyapong, Piyada

doi:10.17660/actahortic.2009.827.107

Cited by 5 publications

(10 citation statements)

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“…Eighty-five F 1 hybrids (16 'Black Queen x NY88.0517.01', 11 'Black Queen x NY65.0550.04', 22 'Black Queen x NY65.0551.05', 11 'Carolina Black Rose x NY88.0517.01', 9 'Carolina Black Rose x NY65.0550.04', and 16 'Carolina Black Rose x NY65.0551.05') and five parents were assessed for anthracnose resistance by the excised leaf assay described by Tharapreuksapong et al (2009). One representative fungal isolate from Nakhon Ratchasima (Nk4-1) was used for the analysis.…”

Section: Anthracnose Resistance Evaluationmentioning

confidence: 99%

Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines

Poolsawat

Mahanil²,

Laosuwan

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) are two of the major diseases of most grapevine (Vitis vinifera L.) cultivars grown in Thailand. Therefore, breeding grapevines for improved downy mildew and anthracnose resistance is crucial. Factorial crosses were made between three downy mildew and/or anthracnose resistant lines ('NY88.0517.01', 'NY65.0550.04', and 'NY65.0551.05'; male parents) and two or three susceptible cultivars of V. vinifera ('Black Queen', 'Carolina Black Rose', and/or 'Italia'; female parents). F 1 hybrid seedlings were evaluated for downy mildew and anthracnose resistance using a detached/excised leaf assay. For both diseases, the general combining Inheritance of grape downy mildew and anthracnose resistance ability (GCA) variance among male parents was significant, while the variance of GCA among females and the specific combining ability (SCA) variance were not significant, indicating the prevalence of additive over non-additive gene actions. The estimated narrow sense heritabilities of downy mildew and anthracnose resistance were 55.6 and 79.2%, respectively, suggesting that downy mildew/anthracnose resistance gene(s) were highly heritable. The 'Carolina Black Rose x NY65.0550.04' cross combination is recommended for future use.

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Section: Anthracnose Resistance Evaluationmentioning

confidence: 99%

Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines

Poolsawat

Mahanil²,

Laosuwan

et al. 2013

Genet. Mol. Res.

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“…F 1 hybrids of seven crosses were assessed for anthracnose resistance by the excised leaf assay described by Tharapreuksapong et al (2009). Two S. ampelinum singleconidial isolates from Nakhon Ratchasima (Nk4-1) and Ratchaburi (Rc2-1) Provinces as described by Poolsawat et al (2009) were used for the analysis.…”

Section: Anthracnose Resistance Evaluationmentioning

confidence: 99%

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Tantasawat¹,

Poolsawat²,

Prajongjai³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) are two major diseases that severely affect most grapevine (Vitis vinifera) cultivars grown commercially in Thailand. Progress of conventional breeding programs of grapevine for improved resistance to these diseases can be speeded up by selection of molecular markers associated with resistance traits. We evaluated the association between 13 resistance gene analog (RGA)-single-strand conformation polymorphism (SSCP) markers with resistance to downy mildew and anthracnose in 71 segregating progenies of seven cross combinations between susceptible cultivars and resistant lines. F 1 hybrids from each cross were assessed for resistance to downy mildew and anthracnose (isolates Nk4-1 and Rc2-1) under laboratory conditions. Association of resistance traits with RGA-SSCP markers was evaluated using simple linear regression analysis. Three RGA-SSCP markers were found to be significantly correlated with anthracnose resistance, whereas significant correlation with downy mildew resistance was observed for only one RGA-SSCP marker. These results demonstrate the usefulness of RGA-SSCP markers. Four candidate markers with significant associations to resistance to these two major diseases of grapevine were identified. However, these putative associations between markers and resistance need to be verified with larger segregating populations before they can be used for marker-assisted selection.

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“…(2009), using six primers; RAPD‐1 (5′GGCACTGAGG3′), OPA‐1 (5′CAGGCCCTTC3′), OPA‐2 (5′TGCCGAGCTG3′), OPA‐3 (5′AGTCAGCCAC3′), MUNG‐1 (5′GGTGCGGAA3′) and MUNG‐2 (5′GTAGACCCGT3′). Only repeatable DNA bands from 2 to 4 experiments were used for genetic similarity analysis and phylogenetic tree construction following Tharapreuksapong et al. (2009).…”

Section: Methodsmentioning

confidence: 99%

“…Only repeatable DNA bands from 2 to 4 experiments were used for genetic similarity analysis and phylogenetic tree construction following Tharapreuksapong et al (2009).…”

Section: Molecular Characterizationmentioning

confidence: 99%

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Genetic Diversity and Pathogenicity Analysis of Sphaceloma ampelinum Causing Grape Anthracnose in Thailand

Poolsawat

Tharapreuksapong

Wongkaew

et al. 2010

Journal of Phytopathology

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Anthracnose is one of the major diseases affecting grape (Vitis vinifera L.) cultivars in Thailand. Isolates of Sphaceloma ampelinum, the anamorph stage of Elsinoe ampelina, were collected from various regions of Thailand. Nineteen single-conidial isolates were evaluated for differences in conidial morphology, DNA patterns and pathogenicity. These isolates could not be unambiguously distinguished based on conidial morphology; however, they were genetically differentiated using random amplified polymorphic DNA markers. Cluster analysis by the unweighted paired grouped mean arithmetic average classified these isolates into four groups. Pathogenicity analysis using nine grape genotypes and five S. ampelinum isolates showed that ÔWilcox321Õ and ÔIllinois547-1 were highly resistant to all isolates, suggesting their usefulness as resistant sources in future breeding programmes.

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Molecular, Morphological, and Pathogenicity Characterization of Sphaceloma Ampelinum Isolates From Thailand

Cited by 5 publications

References 0 publications

Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines

Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Genetic Diversity and Pathogenicity Analysis of Sphaceloma ampelinum Causing Grape Anthracnose in Thailand

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