Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3

Danagoudar, Ananda; Joshi, Chandrashekhar G.; Kumar, Rahul; Poyya, Jagadeesha; Nivya, T; Hulikere, M. Manjunath; Appaiah, K. A. Anu

doi:10.1080/21501203.2017.1281358

Cited by 22 publications

(7 citation statements)

References 49 publications

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“…As shown in Figure 4, most fermentation experiments produced colored compounds, indicating that the production was affected by the culture media constituents and the evaluation conditions. Thus, the use of PDB promotes the formation of colored compounds in endophytes, as reported by several other studies (Danagoudar et al, 2017;Xu et al, 2018).…”

Section: Primary Screeningsupporting

confidence: 69%

Evaluation of the Antibacterial Activity of Crude Extracts Obtained From Cultivation of Native Endophytic Fungi Belonging to a Tropical Montane Rainforest in Colombia

et al. 2021

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Bioactive secondary metabolite production from endophytic fungi has gained a recurring research focus in recent decades as these microorganisms represent an unexplored biological niche for their diverse biotechnological potential. Despite this focus, studies involving tropical endophytes remain scarce, particularly those isolated from medicinal plants of these ecosystems. In addition, the state of the art of the pharmaceutical industry has experienced stagnation in the past 30years, which has pushed pathogenic infections to get one step ahead, resulting in the development of resistance to existing treatments. Here, five fungal endophytes were isolated from the medicinal plant Otoba gracilipes (Myristicaceae), which corresponded to the genera Xylaria and Diaporthe, and screened to demonstrate the promissory potential of these microorganisms for producing bioactive secondary metabolites with broad-spectrum antibacterial activities. Thus, the evaluation of crude organic extracts obtained from the mycelia and exhaust medium allowed the elucidation of Xylaria sp. and Diaporthe endophytica potential toward providing crude extracellular extracts with promising bioactivities against reference strains of Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923), according to the determined half-maximum inhibitory concentration (IC50) with values down to 3.91 and 10.50mg/ml against each pathogen, respectively. Follow-up studies provided insights into the polarity nature of bioactive compounds in the crude extracts through bioactivity guided fractionation using a polymeric resin absorbent alternative extraction procedure. In addition, evaluation of the co-culturing methods demonstrated how this strategy can enhance endophytes biosynthetic capacity and improve their antibacterial potential with a 10-fold decrease in the IC50 values against both pathogens compared to the obtained values in the preliminary evaluations of Xylaria sp. and D. endophytica crude extracts. These results support the potential of Colombian native biodiversity to provide new approaches concerning the global emergence of antibiotics resistance and future production of undiscovered compounds different from the currently used antibiotics classes and simultaneously call for the value of preserving native habitats due to their promising ecosystemic applications in the biotechnological and pharmaceutical industries.

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Section: Primary Screeningsupporting

confidence: 69%

Evaluation of the Antibacterial Activity of Crude Extracts Obtained From Cultivation of Native Endophytic Fungi Belonging to a Tropical Montane Rainforest in Colombia

et al. 2021

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“…It is present in plant seeds and leaves as a component of lignocelluloses, conferring rigidity to the cell wall by making the crosslink between polysaccharides and lignin (45,87). Ferulic acid has been detected in and/or isolated from culture extracts of endophytic fungi like Annulohypoxylon stygium (88) and Aspergillus austroafricanus (89). Ferulic acid has been reported to show several pharmacological activities including antioxidant anti-diabetic, antihypertensive, anticancer, antiinflammatory, hepatoprotective, and antimicrobial activities.…”

Section: Discussionmentioning

confidence: 99%

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Eze

Nnanna

Okezie

et al. 2019

The EuroBiotech Journal

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Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of Newbouldia laevis, Ocimum gratissimum, and Carica papaya The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from N. laevis (NL-L1 and NL-L2), one from O. gratissimum (SL-L1), and two from C. papaya (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against S. aureus, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi C. albicans and A. fumigatus HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.

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“…The antioxidant activity, the lower the IC 50 value (Pratiwi et al, 2013). A previous study by Danagoudar et al (2017) showed that Aspergillus austroafricanus of Zingiber officinale had an antioxidant activity with an IC 50 value of 82 ± 0.78 µg mg -1 . The IC 50 of the endophytic isolates of Malaysia accessions were expressed in graphical form, as shown in Figure 2.…”

Section: Antioxidant Activity Test With a Uv-vis Spectrophotometermentioning

confidence: 96%

Potential of Endophytic Fungi Deriving From Asiatic Pennyworth to Produce Antioxidants

Susilowati¹,

Radiastuti

Nurhasni

et al. 2021

Indones. J. Agric. Sci.

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Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by Aspergillus austroafricanus MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and A. oryzae MM 13 (IC50 = 10.29 ppm) from Malaysia accession. A. austroafricanus MB 1 produced more antioxidant compounds (seven) than A. oryzae MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that A. austroafricanus MB 1 and A. oryzae MM 13 could be further developed as sources of antioxidants.

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Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3

Cited by 22 publications

References 49 publications

Evaluation of the Antibacterial Activity of Crude Extracts Obtained From Cultivation of Native Endophytic Fungi Belonging to a Tropical Montane Rainforest in Colombia

Evaluation of the Antibacterial Activity of Crude Extracts Obtained From Cultivation of Native Endophytic Fungi Belonging to a Tropical Montane Rainforest in Colombia

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Potential of Endophytic Fungi Deriving From Asiatic Pennyworth to Produce Antioxidants

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