Molecular Property-Tailored Soy Protein Extraction Process Using a Deep Eutectic Solvent

Abstract

Using soy protein as a model and deep eutectic solvent as the extraction solvent, the effects of key factors on the extraction of soy protein based on monitoring its molecular properties were systematically studied. The conditions for the recovery of soy protein from deep eutectic solvents were explored and optimized using the response surface methodology. In addition, the influence of the deep eutectic solvent on the structure and physicochemical properties of soy protein was studied using various characterization techniques. The results showed that the hydrogen bonding interaction in the deep eutectic solvent could affect the protein extraction, and the hydrophobic interaction also played an important role in the protein extraction. Moreover, the deep eutectic solvent caused protein denaturation and other changes in the protein, thus affecting the further applications of soy proteins. This study should provide reference and guidance for green extraction of other proteins by deep eutectic solvents in laboratory or industry and provide a basis for the utilization of soy or other proteins extracted from such processes.

Application of deep eutectic solvents in protein extraction and purification

“…They compared the extraction efficiency obtained with this mixture to a method using an aqueous solution of sodium hydroxide. The experiment resulted in an extraction yield enhanced by 60%, which ultimately provides another proof of DESs’ suitability for the extraction of proteins from renewable raw biomasses ( Chen et al, 2021 ).…”

“…Convergently, Chen et al (2021) mixed ChCl with glycerol for the extraction of soy protein and observed that DES-based extraction showed a yield higher by 10% than that of the acid-based precipitation method. Soy protein extracted by means of DESs also showed improved heat resistance and better hydrophobicity than native soy protein, indicating that DESs can enhance the physical properties of the proteins they solubilize, and thus, they can be further used as functional ingredients.…”

Deep Eutectic Solvent as Green Solvent in Extraction of Biological Macromolecules: A Review

“…Chen, Chaihu, Yao, Cao, Bi, Lin, and Chen [ 91 ] used CHCL:glycerol DES as the extraction solvent to extract soy proteins and noticed that DES-extraction resulted in approximately 10% higher yield ( w/w ) as compared to the conventional alkali solution acid precipitation method. In addition, the soy proteins extracted by DES exhibit better heat resistance and stronger hydrophobicity than commercial soy proteins, indicating that DES can improve the functional properties of proteins and the extracted proteins may be used as new functional material.…”

Deep eutectic-like solvents: Promising green media for biomass treatment and preparation of nanomaterials

“…Some DESs are easily recovered by adding water, or PEER-REVIEWED REVIEW ARTICLE bioresources.com with vacuum distillation (Luo et al 2022;Tian et al 2017;Shen et al 2019). There are multifarious substances that can be dissolved in DESs, such as metals (Abbott et al 2004), bioactive substances (Wagle et al 2014;Gállego et al 2015), and protein (Chen et al 2021). It is interesting to find that when it is used to extract proteins, DESs have much higher extraction efficiency and recovery rate than water and conventional organic solvents (Nakhle et al 2021).…”