Molecular Recognition-Triggered Aptazyme Sensor Using a Co-MOF@MCA Hybrid Nanostructure as Signal Labels for Adenosine Triphosphate Detection in Food Samples

Abstract

Developing rapid detection technology for adenosine triphosphate (ATP) is crucial in quality supervision and food safety. Herein, an electrochemical aptasensor based on an aptazyme-catalyzed signal amplification strategy is constructed for ATP detection using polyethyleneimine-functionalized molybdenum disulfide (PEI-MoS 2 )/Au@PtPd nanobipyramids (MoS 2 /Au@PtPd NBPs) as a modification material. Additionally, a novel kind of nitrogen-rich covalent organic framework (COF) is prepared using melamine and cyanuric acid (MCA). We synthesize MCA and the Co-based metal organic framework (Co-MOF) as the signal label. Due to the fact that π−π stacking interactions of Co-MOF@MCA can expand the load efficiency and surface concentration of the signal label, the signal response is an order of magnitude higher than that of Co-MOF or MCA as the signal label. Target ATP changes the conformation of the aptazyme, and it becomes activated. With the assistance of metal ions, the signal label is circularly cleaved, causing an amplification of the signal. Among them, MoS 2 /Au@PtPd NBPs have a large specific surface area and good electrical conductivity and can carry substantial DNA strands and amplify the redox signal of methylene blue (MB). Under optimal conditions, the aptasensor can detect ATP from 10 pM to 100 μM with a low limit of detection of 7.37 × 10 −10 μM. Therefore, the novel aptasensor has extensive application prospects in quality supervision and food safety.

Co-MOF@MWCNTs/GCE for the sensitive detection of TBHQ in food samples

“…3D reveals the XPS spectrum of O 1s, and its characteristic peaks at 530.6, 532.5 and 533.8 eV represent the groups associated with the metal oxide bond, coordination lattice oxygen and organic CO bond, respectively. 28…”

“…3D reveals the XPS spectrum of O 1s, and its characteristic peaks at 530.6, 532.5 and 533.8 eV represent the groups associated with the metal oxide bond, coordination lattice oxygen and organic CvO bond, respectively. 28 To obtain a deeper insight into the vibrational modes of the molecules, the non-destructive technique of RS was used to characterize the synthesized materials. Fig.…”

Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis

“…Recently, emerging novel nanomaterials, such as metal–organic frameworks (MOFs), nanoclusters, covalent–organic frameworks, and MXenes have also been reported for food safety assays. Among them, MOFs with diverse skeleton composition, abundant organic ligands, high specific surface area and good biocompatibility have broad application prospects .…”

“…Recently, emerging novel nanomaterials, such as metal− organic frameworks (MOFs), 67 nanoclusters, 68 covalent− organic frameworks, 69 and MXenes 70 skeleton composition, abundant organic ligands, high specific surface area and good biocompatibility have broad application prospects. 71 Ma et al established a real-time platform for seafood freshness monitoring based on MOF and advanced computer technology.…”

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

“…[9][10][11] Among them, gold@platinum palladium nanoparticles (Au@PtPd NPs) have a dendrite morphology and uniform size, and show an excellent peroxidase-mimic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2 ). 12,13 Recently, Au@PtPd NPs were used to detect ATP 14 and tumor biomarkers, 15 but were rarely used to detect bacteria. A recent example was reported to use Au@PtPd NPs in lateral flow immunoassay with direct chromatographic readout, which was verified for quantification of diaminochlorotriazine with a detection limit of 0.7 ng mL −1 .…”