Molecular Resolution Imaging of Dextran Monolayers Immobilized on Silica by Atomic Force Microscopy

Tasker, S.; Matthijs, Gert; Davies, Martyn C.; Roberts, Clive J.; Schacht, Etienne; Tendler, Saul J. B.

doi:10.1021/la951087i

Cited by 30 publications

(22 citation statements)

References 31 publications

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“…Successful reactions include N,N'-dicyclohexylcarbodiimide (DCC) coupling of pepsin, [91] 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) coupling of DNA, [177] the immobilization of pnitrophenyl chloroformate activated dextrans, [178] reaction of compounds containing acidic protons with diazobenzilidenefunctionalized glass slides, [179] and a photoaffinity reaction. [180] Many research groups have employed a two-step approach, where the amine-functionalized SAM was derivatized with a linker molecule after self-assembly to increase the reactivity of the monolayer.…”

Section: Immobilization Of Biomoleculesmentioning

confidence: 99%

Engineering Silicon Oxide Surfaces Using Self‐Assembled Monolayers

2005

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Although a molecular monolayer is only a few nanometers thick it can completely change the properties of a surface. Molecular monolayers can be readily prepared using the Langmuir-Blodgett methodology or by chemisorption on metal and oxide surfaces. This Review focuses on the use of chemisorbed self-assembled monolayers (SAMs) as a platform for the functionalization of silicon oxide surfaces. The controlled organization of molecules and molecular assemblies on silicon oxide will have a prominent place in "bottom-up" nanofabrication, which could revolutionize fields such as nanoelectronics and biotechnology in the near future. In recent years, self-assembled monolayers on silicon oxide have reached a high level of sophistication and have been combined with various lithographic patterning methods to develop new nanofabrication protocols and biological arrays. Nanoscale control over surface properties is of paramount importance to advance from 2D patterning to 3D fabrication.

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Section: Immobilization Of Biomoleculesmentioning

confidence: 99%

Engineering Silicon Oxide Surfaces Using Self‐Assembled Monolayers

2005

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“…Zu diesen zählen die N,N'-Dicyclohexylcarbodiimid(DCC)-Kupplung von Pepsin, [91] die 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimid(EDC)-Kupplung von DNA, [177] die Immobilisierung von Chlorameisensäure-4-nitrophenylester-aktivierten Dextranen, [178] Reaktionen von sauren HAtomen enthaltenden Verbindungen mit Diazobenzilidenfunktionalisierten Glassubstraten [179] sowie eine Photoaffinitätsreaktion. [180] Oftmals wurden zweistufige Verfahren eingesetzt, bei denen nach erfolgter Selbstorganisation die Amin-SAM mit einem Linker-Molekül derivatisiert wurde, um die Reaktivität der Monoschicht zu erhöhen.…”

Section: Sensorschichtenunclassified

Gestaltung der Siliciumoxidoberfläche durch selbstorganisierte Monoschichten

2005

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Nur wenige Nanometer dicke molekulare Monoschichten können die Eigenschaften von Oberflächen vollkommen verändern. Die Herstellung dieser molekularen Monoschichten kann leicht durch die Langmuir‐Blodgett‐Methode oder durch chemische Adsorption (Chemisorption) an Metall‐ und Oxidoberflächen erfolgen. Dieser Aufsatz konzentriert sich auf chemisorbierte selbstorganisierte Monoschichten (SAMs) als Plattformen für die Funktionalisierung von Siliciumoxidoberflächen. Die Organisation von Molekülen und molekularen Aufbauten auf Siliciumoxid wird in der “Bottom‐Up”‐Nanofabrikation einen außerordentlichen Platz einnehmen und könnte Gebiete wie die Nanoelektronik und die Biotechnologie in naher Zukunft revolutionieren. In den letzten Jahren wurden selbstorganisierte Monoschichten auf Siliciumoxid so weit entwickelt und mit verschiedenen lithographischen Methoden kombiniert, dass neue Nanofabrikationsstrategien und biologische Arrays entwickelt werden können. Will man Fortschritte auf dem Weg von der Bildung zweidimensionaler Muster hin zur dreidimensionalen Fabrikation erzielen, ist die Kontrolle von Oberflächeneigenschaften im Nanometerbereich von größter Bedeutung.

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“…Furthermore, interaction of the sample with areas of the probe tip other than the apex may lead to ''tip self-imaging'' and prevent optimum profiling of the edges of molecular features. 40 The results indicate that AFM can observe the aggregates and single chains simultaneously, since AFM is a sensitive instrument and the desired concentration is extremely dilute. In view of the above result, the size and shape of the aggregates and individual molecules of hyperbranched polysaccharide in two solutions have been determined by using SEC-LLS combined with microscopy.…”

Section: Molecular Morphologymentioning

confidence: 94%

Determination of molecular size and shape of hyperbranched polysaccharide in solution

Tao

Zhang

2006

Biopolymers

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The chemical structure of a water-soluble polysaccharide, coded as TM3b, extracted from sclerotia of Pleurotus tuber-rigium was analyzed to be a hyperbranched beta-D-glucan with beta-(1-->6), beta-(1-->4), and beta-(1-->3)-linked residues, with degree of branching (DB) of 57.6%. The results from size-exclusion chromatography combined with laser light scattering (SEC-LLS) revealed that the hyperbranched polysaccharide easily aggregated in 0.15 M aqueous NaCl, whereas it dispersed as individual chains in DMSO. The weight-average molecular weight (M(w)), radius of gyration, intrinsic viscosity, and chain density of TM3b in DMSO and in 0.15 M aqueous NaCl were measured with SEC-LLS, LLS, and viscometry. The results indicated that single chains and aggregates with aggregation number of 12 coexisted in the aqueous solution, whereas individual molecules of TM3b occurred in DMSO. In view of the molecular parameters, the aggregates in aqueous solution exhibited more compact chain structure than the individual molecules in DMSO. Furthermore, transmission electron microscopy and atomic force microscopy showed that all of the aggregates and individual molecules exhibited spherical particles in the solutions. This work provided the valuable information of chain conformation and molecular morphology of the hyperbranched polysaccharide in different solvents.

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Molecular Resolution Imaging of Dextran Monolayers Immobilized on Silica by Atomic Force Microscopy

Cited by 30 publications

References 31 publications

Engineering Silicon Oxide Surfaces Using Self‐Assembled Monolayers

Engineering Silicon Oxide Surfaces Using Self‐Assembled Monolayers

Gestaltung der Siliciumoxidoberfläche durch selbstorganisierte Monoschichten

Determination of molecular size and shape of hyperbranched polysaccharide in solution

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