Molecular study of intraspecific differences among Sauropus androgynus (L.) Merr. from Indonesia revealed by ITS region variability

Abstract

DNA sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region was exploited for its applications in the authentication of Sauropus androgynus (L.) Merr. as raw-material herbal medicine and supplement. Leaf samples from 12 different geographic locations in Indonesia were collected. Twelve DNA sequences from the samples were analysed. The sequences were trimmed before sequence alignment. The sequences ranged from 626 to 664 bp in length after trimming. Based on the multiple sequence alignment, a phylogenetic tree was generated using the MrBayes program. It consisted of two groups: one group comprised sequences from Trawas and the other one comprised sequences from Batu, Trawas and Surabaya. These results strongly suggest that ITS sequence analysis is a valuable tool for distinguishing intraspecific differences among S. androgynus cultivars from Indonesia.

Molecular characterization of red ginger varieties (Zingiber officinale Roxb. var. rubrum) by DNA markers

“…The DNA extraction from red ginger rhizome was performed using a specific set of reagents provided in the Nucleospin Plant II kit (Macherey-Nagel GmbH & Co. Germany) according to the previous research (Yunita et al 2016). A quantity of 100 milligrams of plant powder was put into a sterile tube.…”

“…The rhizome samples of red ginger were stored in a refrigerator set at a temperature of -20°C, following the methodology employed by Yunita et al (2016), until the commencement of the sample analysis procedure. The sample is cooled in this manner due to the inactivation of enzymes responsible for the degradation of nucleic acids and plant metabolites at a temperature of -20°C.…”

Karakterisasi Morfologis dan Molekuler Varietas Kedelai (Glycine max) untuk Identifikasi Bahan Baku Obat Tradisional Indonesia

“…Hal ini diduga karena adanya kolom Nucleospin hijau pada kit. Nucleospin hijau ini terdiri dari kolom dengan fase diam silika yang dapat menahan DNA melalui terbentuknya ikatan antara DNA dengan silika (23).…”

“…Berdasarkan hasil optimasi penambahan PE dilakukan dua tahap elusi masing-masing 25 µl. Tahapan elusi yang dilakukan dua kali biasanya menghasilkan DNA yang lebih banyak dibandingkan dengan elusi yang dilakukan satu kali dengan volume total yang sama (17,23).…”

Characterization of Spray Dried Extract Katuk (Sauropus androgynus)

“…Unfortunately, few literature reviews have discussed methods for distinguishing S. androgynus cultivars based on their morphological characteristics. However, previous studies revealed that chromatography (Yunita et al, 2019) and several DNA-based methods such as random amplified polymorphic DNA (RAPD) (Yunita & Sulisetiorini, 2013) and sequencing of internal transcribed spacers (ITS) (Yunita et al, 2016) successfully discriminate between geographically sensitive S. androgynus cultivars.…”