Molecular taxonomy and its application to ecological studies of Pythium species

Kageyama, Koji

doi:10.1007/s10327-014-0526-2

Cited by 35 publications

(16 citation statements)

References 100 publications

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“…are no longer considered to be true fungi; instead, they were found to be more closely related to algae [ 37 ]. The class Oomycetes differ from true fungi in that they have coenocytic hyphae, a diploid vegetative stage, cell wall content of β-glucan and cellulose, biflagellate zoospores with tinsel, and whiplash flagella [ 37 , 38 ]. The Pythium genus contains species that are pathogenic to plants [ 39 , 40 , 41 ], animals [ 42 ], algae [ 43 ], and other fungi [ 44 ].…”

Section: Pythium Species: Causal Agent Of Damping-off and Root Rotmentioning

confidence: 99%

“…Pythium spp.inhabit the soil either as a propagule (Zoospore, oospore, or sporangia) or in the mycelial form. The oospores are the primary survival structure and infecting unit of the many species of the Pythium genus in the soil, which can stay viable in the soil for long durations [ 37 , 55 ]. However, sporangia of some Pythium species have also been reported to be survival propagules in soil [ 56 , 57 ].…”

Section: Pythium Species: Causal Agent Of Damping-off and Root Rotmentioning

confidence: 99%

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Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Arora

Sharma

et al. 2021

Microorganisms

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Capsicum annuum L. is a significant horticulture crop known for its pungent varieties and used as a spice. The pungent character in the plant, known as capsaicinoid, has been discovered to have various health benefits. However, its production has been affected due to various exogenous stresses, including diseases caused by a soil-borne pathogen, Pythium spp. predominantly affecting the Capsicum plant in younger stages and causing damping-off, this pathogen can incite root rot in later plant growth stages. Due to the involvement of multiple Pythium spp. and their capability to disperse through various routes, their detection and diagnosis have become crucial. However, the quest for a point-of-care technology is still far from over. The use of an integrated approach with cultural and biological techniques for the management of Pythium spp. can be the best and most sustainable alternative to the traditionally used and hazardous chemical approach. The lack of race-specific resistance genes against Pythium spp. can be compensated with the candidate quantitative trait loci (QTL) genes in C. annuum L. This review will focus on the epidemiological factors playing a major role in disease spread, the currently available diagnostics in species identification, and the management strategies with a special emphasis on Pythium spp. causing damping-off and root rot in different cultivars of C. annuum L.

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Section: Pythium Species: Causal Agent Of Damping-off and Root Rotmentioning

confidence: 99%

Section: Pythium Species: Causal Agent Of Damping-off and Root Rotmentioning

confidence: 99%

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Arora

Sharma

et al. 2021

Microorganisms

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“…The genus Pythium is highly diverse, containing more than 140 species, and has a cosmopolitan distribution present in terrestrial and aquatic habitats (Kageyama, 2014; Kirk et al , 2008; Tojo & Newsham, 2012). Members of this genus exhibit saprophytic and parasitic life styles (Hong & Moorman, 2005; Martin & Loper, 1999; Nechwatal et al , 2008).…”

Section: Introductionmentioning

confidence: 99%

Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves

Bouket

Arzanlou

Tojo

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Pythium kandovanense sp. nov. (ex-type culture CCTU 1813T = OPU 1626T = CBS 139567T) is a novel oomycete species isolated from Lolium perenne with snow rot symptoms in a natural grassland in East-Azarbaijan province, Iran. Phylogenetic analyses based on sequence data from internal transcribed spacer (ITS)-rDNA, coxI and coxII mitochondrial genes clustered our isolates in Pythium group E as a unique, well supported clade. Pythium kandovanense sp. nov. is phylogenetically and morphologically distinct from the other closely related species in this clade, namely Pythium rostratifingens and Pythium rostratum. Pythium kandovanense sp. nov. can be distinguished from these two species by its cylindrical sporangia and lower temperatures for optimum and maximum growth rate. The development of zoospores released through a shorter discharge tube is an additional morphological feature which can be used to differentiate Pythium kandovanense sp. nov. from Pythium rostratifingens. Laboratory inoculation tests demonstrated the pathogenicity of Pythium kandovanense sp. nov. to L. perenne under wet cold (0–3 °C) conditions.

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“…These reactions require species-specific primers based on the ribosomal DNA internal transcribed spacer (ITS) region, cytochrome oxidase II, and β-tubulin genes (Villa et al 2006;Schroeder et al 2013;Kageyama 2014). Popular detection method for P. inflatum mainly based on booster PCR (Wang et al 2003b).…”

Section: Introductionmentioning

confidence: 99%

“…The genus Pythium consists of more than 140 validly described species (Kageyama 2014). The reliable identification of Pythium spp.…”

Section: Introductionmentioning

confidence: 99%

Rapid and quantitative detection of Pythium inflatum by real-time fluorescence loop-mediated isothermal amplification assay

Cao

et al. 2015

Eur J Plant Pathol

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Pythium inflatum is the causal agent of Pythium maize stalk rot, which is one of the most devastating diseases of maize (Zea mays L.). P. inflatum is currently a major concern in global maize production. To the best of our knowledge, no effective resistance to P. inflatum is known in maize, and no effective measures have been reported for the control of this pathogen once maize plants have been infected. Early and accurate detection of P. inflatum is essential to guide maize planting and to protect maize production. A real-time fluorescence loopmediated isothermal amplification (RealAmp) assay was developed for the rapid quantitative detection of P. inflatum in soil. The detection limit of the RealAmp assay was approximately 0.1 pg/μl plasmid DNA when mixed with extracted soil DNA or 10 3 spores/g of artificially infested soil. No cross-reactions with other related pathogens were observed. Results of the RealAmp assay for quantifying the genomic DNA of P. inflatum were confirmed by testing with artificially and naturally infested samples. The quantification of the soil-borne pathogen DNA of P. inflatum in naturally infested samples was not significantly different compared with classic real-time PCR (P < 0.05). Additionally, the RealAmp assay could be detected via an improved closed-tube visual detection system by adding SYBR Green I fluorescent dye to the inside of the lid prior to amplification. Consequently, the inhibitory effects of the stain on DNA amplification were avoided. Therefore, the assay could be used more conveniently in the field as a simple, rapid, and effective technique and has the potential to become an alternative tool for detecting and monitoring P. inflatum in the field.

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Molecular taxonomy and its application to ecological studies of Pythium species

Cited by 35 publications

References 100 publications

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Pythium Damping-Off and Root Rot of Capsicum annuum L.: Impacts, Diagnosis, and Management

Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves

Rapid and quantitative detection of Pythium inflatum by real-time fluorescence loop-mediated isothermal amplification assay

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