Molecular Techniques and Current Research Approaches

Walker, Laura M.; Hoppe, Thomas; Silliker, Margaret E.

doi:10.1016/b978-0-12-805089-7.00005-6

Cited by 4 publications

(5 citation statements)

References 138 publications

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“…Denaturing gradient gel electrophoresis (DGGE) is a molecular technique that can be incorporated into the PCR to increase the separation of mixture of DNA fragments in complex microbial communities and thus enhance the wound infection identification. 83 Oates et al 84 made use of PCR-DGGE to access the microbial diversity of diabetic chronic foot wounds, involving tissue debridement samples from 26 chronic diabetic foot wounds. Semiquantitative culture counts and Gram staining were performed as reference.…”

Section: Molecular Methods (Pcr)mentioning

confidence: 99%

Detecting bacterial infections in wounds: a review of biosensors and wearable sensors in comparison with conventional laboratory methods

Salleh

Tanaka

Sutarlie

2022

Analyst

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Section: Molecular Methods (Pcr)mentioning

confidence: 99%

Detecting bacterial infections in wounds: a review of biosensors and wearable sensors in comparison with conventional laboratory methods

Salleh

Tanaka

Sutarlie

2022

Analyst

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“…Although there is a long tradition of myxomycete studies, especially intense since the last decade of the19th century when the first monograph of the group appeared (Rostafiński 1874(Rostafiński , 1875, molecular methods were introduced in studies of this group relatively late in comparison with other eukaryotes (Walker et al 2017). Consequently, the bright-spored group of Myxomycetes still suffers from scarcity of reference DNA sequences (Fiore-Donno et al 2013;Schnittler et al 2017) necessary to elucidate the phylogenetic position of species and find a proper delimitation of genera.…”

Section: Phylogenetic Affinity Of the Andean Endemics In The Context Of The Phylogeny Of Trichiales-mentioning

confidence: 99%

Nivicolous Trichiales from the austral Andes: unexpected diversity including two new species

et al. 2020

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Nivicolous myxomycetes are a group of amoebozoan protists dependent on long-lasting snow cover worldwide. Recent fine-scale analysis of species diversity from the austral Andes revealed high intraspecific variability of most taxa, suggesting independent evolutionary processes and significant differences in species compositions between the Northern (NH) and Southern (SH) Hemispheres. The present study is the second part of this analysis based on representatives of Trichiales. A total of 173 South American collections were studied based on morphological and molecular data, and 15 taxa have been identified. Two of them, Hemitrichia crassifila and Perichaena patagonica, are proposed as new species confirmed by a phylogeny of Trichiales. However, their affinity to the genera in which they are proposed are not confirmed due to polyphyletic character of all genera of Trichiales. Four species, Dianema subretisporum, Trichia contorta var. karstenii, T. nivicola, and T. sordida, are reported for the first time from the Southern Hemisphere. One species, T. alpina, is new for Argentina. Additionally, we provide the first record of Perichaena megaspora from Chile. Specimen frequency and species diversity of Trichiales found at nivicolous localities in the austral Andes are unexpectedly high, exceeding those of Stemonitidales, the most numerous group in the Northern Hemisphere, where Trichiales play a marginal role. By contrast, Trichiales appear the main component of nivicolous assemblages in the Andes. Results of the present work, together with the earlier analysis of Stemonitidales, indicate that the Andes constitute an exceptionally important evolutionary hot spot for nivicolous myxomycetes characterized by an outstanding species diversity.

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“…The 1960s marked the beginning of studies with molecular approaches to myxomycetes studies (Fig. 4), with the use of isozyme patterns in the investigation of relationships between closely related species (Walker et al 2017). However, the use of the moist chamber technique remains the most widely used, especially for fimicolous myxomycetes, where there is a need to induce the appearance of sporangia to attest to the occurrence record.…”

Section: Discussionmentioning

confidence: 99%

“…It is recommended in some cases, to extend the moist chamber study period to up to 6 months. Since Gilbert and Martin (1933), whose work is often attributed as a diffuser of the technique, the taxonomy of myxomycetes is practically based on the traditional method of morphologically defined species, due to the scarcity of welldefined molecular techniques, inputs, and resources, such as specific DNA isolation kits for dealing with environmental myxomycetes samples (Walker et al 2017). Therefore, misidentified records are understandable to some extent.…”

Section: T Papillatamentioning

confidence: 99%

“…ncbi.nlm.nih.gov/genbank/), has increased in recent years, there is still a shortage of sequences of fimicolous species. Walker et al (2017) describe the main techniques used for the ecological and biological study that, added to the new approach proposed by Janik et al (2020), should contribute to improving the knowledge of the species of fimicolous myxomycetes. Among these technologies, the terminal restriction fragment length polymorphism (TRFLP) with myxomycete SSU, denaturing gradient gel electrophoresis (DGGE) and highthroughput sequencing (e.g.…”

Section: T Papillatamentioning

confidence: 99%

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Fimicolous myxomycetes: overview of their global distribution and scientific production

Calaça

Araújo

Cacialli³

et al. 2020

Biologia

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Myxomycetes (Amoebozoa) can be found on a wide range of substrates and among these, the dung of several animal species, primarily herbivorous, in which case they are considered fimicolous. Dung can be a favourable substrate for myxomycete due its relatively high content of water, nutrients and microorganisms. Despite efforts to study fimicolous myxomycetes, there are still informational gaps on the geographical distribution and microhabitat details. Also, scientometric information on these organisms is scarce. This work was aimed to compile the occurrence of fimicolous myxomycetes, from published literature, for the period between 1900 and 2017, resulting in an update on their biogeographical and ecological information. Scientific production involving fimicolous myxomycetes is also discussed. Ninety-eight articles were retrieved, from which authors recorded a total of 544 occurrences classified in 126 myxomycetes species. These records were geographically associated with 51 countries, located primarily on the northern hemisphere. Most occurrences were reported on herbivore dung, mainly from cattle, horse, deer, rabbit/hare, sheep and camel. Arcyria cinerea, Didymium difforme, D. iridis, D. squamulosum, Fuligo cinerea, Kelleromyxa fimicola, Licea tenera, Perichaena chrysosperma, P. corticalis, P. depressa, Physarum apiculosporum, Ph. compressum, and Ph. didermoides were the most frequent species, with at least 10 records each. Despite an increase in scientific production on fimicolous myxomycetes during the studied period, the number of researchers dedicated to this group is low and the inter-institutional collaboration could be improved. It was observed that most authors have produced only one publication, claimed not to be specialists on the group and that tropical fimicolous myxomycetes have clearly been understudied. It is suggested that thematic networks and methodological standardization in molecular studies could increase and improve research on fimicolous myxomycetes. We highlight the importance of the inter-institutional partnership between researchers interested in the study of fimicolous myxomycetes, once access to these technologies is limited for many researchers, especially those in underdeveloped countries, such as Latin American, the formation of collaborative networks may facilitate the application of molecular approaches.

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Molecular Techniques and Current Research Approaches

Cited by 4 publications

References 138 publications

Detecting bacterial infections in wounds: a review of biosensors and wearable sensors in comparison with conventional laboratory methods

Detecting bacterial infections in wounds: a review of biosensors and wearable sensors in comparison with conventional laboratory methods

Nivicolous Trichiales from the austral Andes: unexpected diversity including two new species

Fimicolous myxomycetes: overview of their global distribution and scientific production

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