Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment

Rastogi, Gurdeep; Sani, Rajesh K.

doi:10.1007/978-1-4419-7931-5_2

Cited by 212 publications

(174 citation statements)

References 71 publications

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“…The development of biotech brought revolutionary progress in the studies of microbial diversity, which made us be able to learn microbial diversity by checking DNA sequence [9]. Although the method based microbial DNA is a more accurate way to acquire the information of diversity [10], yet in International Microbiology Congress 2014, some scholars claimed that Biolog technique based on living microorganism is a more practical way to show microbial function in ecosystem.…”

Section: Introductionmentioning

confidence: 99%

Application and Optimization of Biolog EcoPlates in Functional Diversity Studies of Soil Microbial Communities

Poudel

2015

MATEC Web of Conferences

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Section: Introductionmentioning

confidence: 99%

Application and Optimization of Biolog EcoPlates in Functional Diversity Studies of Soil Microbial Communities

Poudel

2015

MATEC Web of Conferences

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“…Corresponding neighbour joining (NJ) tree showed clearly that strain CLB32 fell into the group of Gliomastix polychroma with strong support (Figure 3). ITS rDNA sequence profiling is one of the cost-effective tools that enable the researchers to identify the complex microbial communities in species level at exceptional depth and resolution (Rastogi and Sani 2011). The number of previously phylogenetically unstudied fungi is large (Summerbell et al 2011); however, morphological identification has long been the basis and tradition, which is, beyond doubt, of great importance (Wang et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Molecular profiling and antimicrobial potential of endophyticGliomastix polychromaCLB32 inhabitingCombretum latifoliumBlume

et al. 2015

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Fungal endophytes as a source of bioactive metabolites have led to the development of pharmaceutical products finding new applications. In a survey of endophytic fungal biodiversity, an antimicrobial endophytic strain CLB32 was isolated from the leaf of Combretum latifolium Blume (Combretaceae) from the Western Ghats of Southern India. CLB32 was then identified as Gliomastix polychroma (KR704576) by morphological and phylogenetic analysis based on internal transcribed spacer (ITS) nuclear rDNA and intervening 5.8S rRNA gene. CLB32 here constituted the first report on incidence of endophytic fungi from C. latifolium Blume. Ethyl acetate fraction of strain CLB32 was evaluated for antimicrobial activity by disc diffusion assay. Secondary metabolites produced effectively inhibited methicillin-resistant Staphylococcus aureus (18.33 ± 0.33 mm), Pseudomonas aeruginosa (14.66 ± 0.33 mm) and Candida albicans (14.00 ± 0.57 mm). Biosynthesis of these antimicrobial compounds was detected by analytical TLC-bioautography method as depicted by zone of inhibition on intensive the band. These findings suggest that G. polychroma CLB32, as a producer of natural antimicrobial drugs, could help to combat against multidrug-resistant infections and also provide baseline information for industrial applications.

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“…Microorganisms that inhabit the soil make up very diverse populations and with recent advances in molecular biology even more complex levels of diversity have been discovered (Rastogi and Sani 2011).…”

Section: Molecular Techniques For the Study Of Functional Groups Of Smentioning

confidence: 99%

“…The combined use of different molecular techniques based on the small-subunit rRNA has generated important data on the diversity, structure and dynamics of microbial communities (Rastogi and Sani 2011;Rajendhran and Gunasekaran 2008). For example, DNA-based molecular studies have shown that clone libraries constructed from soil can be composed almost entirely of single members of microbial communities (Zhou et al 2002).…”

Section: Molecular Techniques For the Study Of Functional Groups Of Smentioning

confidence: 99%

Plant and microbe genomics and beyond: potential for developing a novel molecular plant nutrition approach

2015

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Classical soil science approaches have enabled us to establish basic principles of how the soil system functions and have answered numerous practical agricultural application questions. In recent years, efforts have been refocused on better understanding, managing and benefiting from this system that contains one of the most complex biological communities of the planet. Soil biology is seen as being at the center of scientific research of this century, with novel research objectives and goals being set. In addition, plant nutrition has enabled us to understand nutrient uptake, transport and mobilization mechanisms in plants, and both disciplines have converged on the area of microorganism-mediated plant nutrition. The challenge for these scientific areas is to identify microorganism communities and the roles they play in their habitats, as well as the mechanisms that plants have to make better use of nutrients. Genomics and metagenomics, along with microbiological techniques, are contributing greatly to advances in our understanding of living systems that exist in the soil and their interaction with plants. For its part, molecular plant nutrition has made significant progress in understanding the use of nutrients by plant cells, and has identified molecular mechanisms that can improve nutrient use efficiency. Together, molecular soil microbiology and molecular plant nutrition are projected to be a driving force in agriculture and sustainable food production in the coming years. Herewith, we aim to integrate recent literature on basic and applied research concerning plant and microbe genomics in terms of their potential for developing a novel molecular plant nutrition approach, with special emphasis on nitrogen, potassium and phosphorous as the major macronutrients for crop plants.

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Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment

Cited by 212 publications

References 71 publications

Application and Optimization of Biolog EcoPlates in Functional Diversity Studies of Soil Microbial Communities

Application and Optimization of Biolog EcoPlates in Functional Diversity Studies of Soil Microbial Communities

Molecular profiling and antimicrobial potential of endophyticGliomastix polychromaCLB32 inhabitingCombretum latifoliumBlume

Plant and microbe genomics and beyond: potential for developing a novel molecular plant nutrition approach

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