Molecular Tools and Techniques for Understanding the Microbial Community Dynamics of Vermicomposting

Meghvansi, Mukesh K.; Chaudhary, K. K.; Khan, Mohammad Haneef; Siddiqui, Sazada; Varma, Ajit

doi:10.1007/978-3-030-39173-7_7

Cited by 1 publication

(2 citation statements)

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“…Single-strand conformation polymorphism (SSCP) is based on the separation of DNA fragments such as denatured PCR products on a nondenaturing polyacrylamide gel resulting in single-stranded DNA. The separation is based on the folded conformation of single-stranded DNA that affects electrophoretic movement resulted in measurable differences between sequences [12]. SSCP is much more straightforward compared to DGGE fingerprinting since it does not use any GC clamped primers or a gradient gel.…”

Section: Single-strand Conformation Polymorphism (Sscp)mentioning

confidence: 99%

“…The microbial community structure is crucial in determining the compost maturity as it will modify and evolve during the composting process. Several methods are used to determine microbial community structure in composting such as the conventional biochemical tests, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) [10], terminal restriction fragment length polymorphism (T-RFLP) [11] and single strand-conformation polymorphism (SSCP) [12], microarray analysis [13] and next-generation sequencing (NGS) based 16S ribosomal ribonucleic acid (rRNA) sequencing [14].…”

Section: Introductionmentioning

confidence: 99%

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Food Waste Composting and Microbial Community Structure Profiling

et al. 2020

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Over the last decade, food waste has been one of the major issues globally as it brings a negative impact on the environment and health. Rotting discharges methane, causing greenhouse effect and adverse health effects due to pathogenic microorganisms or toxic leachates that reach agricultural land and water system. As a solution, composting is implemented to manage and reduce food waste in line with global sustainable development goals (SDGs). This review compiles input on the types of organic composting, its characteristics, physico-chemical properties involved, role of microbes and tools available in determining the microbial community structure. Composting types: vermi-composting, windrow composting, aerated static pile composting and in-vessel composting are discussed. The diversity of microorganisms in each of the three stages in composting is highlighted and the techniques used to determine the microbial community structure during composting such as biochemical identification, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), terminal restriction fragment length polymorphism (T-RFLP) and single strand-conformation polymorphism (SSCP), microarray analysis and next-generation sequencing (NGS) are discussed. Overall, a good compost, not only reduces waste issues, but also contributes substantially to the economic and social sectors of a nation.

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Section: Single-strand Conformation Polymorphism (Sscp)mentioning

confidence: 99%