Molecular tools for sterile sperm detection to monitor <i>Ceratitis capitata</i> populations under <scp>SIT</scp> programmes

Juan-Blasco, María; Sabater-Muñoz, Beatriz; Argilés, Rafael; Jacas, J. A.; Castañera, Pedro; Urbaneja, Alberto

doi:10.1002/ps.3448

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…Therefore, continuing research into attractants is important to gain knowledge into the incidence and distribution of pest populations, and to easily and reliably detect released versus wild insects in the field (e.g., Juan‐Blasco et al. ). This includes theoretical and empirical investigations of the effects of environmental factors (Dufourd and Dumont ).…”

Section: A Perspective From Evolutionary Biologymentioning

confidence: 99%

“…Trapping and detection methods are also key to successful insect control and just as important to the successful implementation of genetic control methods as the basic genetic technology itself. Therefore, continuing research into attractants is important to gain knowledge into the incidence and distribution of pest populations, and to easily and reliably detect released versus wild insects in the field (e.g., Juan-Blasco et al 2013). This includes theoretical and empirical investigations of the effects of environmental factors (Dufourd and Dumont 2013).…”

Section: A Perspective From Evolutionary Biologymentioning

confidence: 99%

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Evolutionary biology and genetic techniques for insect control

Leftwich

Bolton

Chapman

2015

Evolutionary Applications

104

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The requirement to develop new techniques for insect control that minimize negative environmental impacts has never been more pressing. Here we discuss population suppression and population replacement technologies. These include sterile insect technique, genetic elimination methods such as the release of insects carrying a dominant lethal (RIDL), and gene driving mechanisms offered by intracellular bacteria and homing endonucleases. We also review the potential of newer or underutilized methods such as reproductive interference, CRISPR technology, RNA interference (RNAi), and genetic underdominance. We focus on understanding principles and potential effectiveness from the perspective of evolutionary biology. This offers useful insights into mechanisms through which potential problems may be minimized, in much the same way that an understanding of how resistance evolves is key to slowing the spread of antibiotic and insecticide resistance. We conclude that there is much to gain from applying principles from the study of resistance in these other scenarios – specifically, the adoption of combinatorial approaches to minimize the spread of resistance evolution. We conclude by discussing the focused use of GM for insect pest control in the context of modern conservation planning under land‐sparing scenarios.

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Section: A Perspective From Evolutionary Biologymentioning

confidence: 99%

Section: A Perspective From Evolutionary Biologymentioning

confidence: 99%

Evolutionary biology and genetic techniques for insect control

Leftwich

Bolton

Chapman

2015

Evolutionary Applications

104

View full text Add to dashboard Cite

show abstract

“…The study was conducted from June 2010 to June 2011 in Montcada (Valencia, Spain), where C. capitata is a prevalent pest (Urbaneja et al , 2009) and subjected to an aw-SIT program. Ten field-cage tests following the same handling methods, experimental set-up, and evaluation described in Juan-Blasco et al (2013 b ) were carried out every ∼ 30 days under different environmental conditions.…”

Section: Methodsmentioning

confidence: 99%

“…In Spain, after years of an area-wide control program based on insecticide applications, an aw-integrated pest management program (aw-IPM) has been implemented to reduce the long-term costs of its control. Mass trapping, biological control, selective pesticide applications, chemosterilization, and aw -SIT are used in combination, as part of this aw-IPM (Sabater-Muñoz et al , 2009; Urbaneja et al , 2009; Navarro-Llopis et al , 2011: Tormos et al , 2012; Juan-Blasco et al , 2013 a , b ). The combination of all these management strategies is aimed at reducing C. capitata populations and consequently the presence of infested fruit and its economic implications.…”

Section: Introductionmentioning

confidence: 99%

Estimating SIT-driven population reduction in the Mediterranean fruit fly,Ceratitis capitata, from sterile mating

Juan-Blasco

Sabater-Muñoz

Plá³

et al. 2014

Bull. Entomol. Res.

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Area-wide sterile insect technique (SIT) programs assume that offspring reduction of the target population correlates with the mating success of the sterile males released. However, there is a lack of monitoring tools to prove the success of these programs in real-time. Field-cage tests were conducted under the environmental conditions of the Mediterranean coast of Spain to estimate: (a) the mating success of sterile Vienna-8 (V8) Ceratitis capitata males using molecular markers and (b) their efficacy to reduce C. capitata populations under six release ratios of wild females to wild males to V8 males (1:0:0, 1:1:0, 1:1:1, 1:1:5, 1:1:10, and 1:1:20). Statistical models were developed to predict: (a) the number of females captured in traps, (b) sperm ID (sterile or not) in spermathecae of the trapped females, and (c) the viable offspring produced, using release ratio and temperature as predictors. The number of females captured was affected by relative humidity. However, its influence in the model was low. Female captures were significantly higher in ratios 1:0:0 compared to ratios where V8 males were released. The proportion of V8 sperm in spermathecae increased with temperature and with the number of V8 males released, but leveled off between ratios 1:1:10 and 1:1:20. In all seasons, except winter (no offspring), viable offspring increased with temperature and was lowest for ratio 1:1:20. For the first time, a strong negative relationship between proportion of V8 sperm detected by molecular tools and C. capitata offspring was established. The models obtained should contribute to enhance the efficacy of SIT programs against this pest.

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“…Consequently, it is very important to distinguish new introductions from failures of the eradication practices within the 322 N. Barr et al treatment zone (Roderick and Villablanca 1996). Also, when Sterile Insect Technique (SIT) is used to suppress outbreaks of a pest, it is crucial that the trapped flies be correctly identified as derived from either the lab source or the pest population to evaluate the effectiveness of the program (Aketarawong et al 2011;Juan-Blasco et al 2013). Given that the released sterile flies are the same species as the targeted population, distinguishing between the two is difficult by trap inspection alone.…”

Section: Introductionmentioning

confidence: 99%