Molecular Typing by Genus-Specific PCR and Rapd Profiling of Diverse<i>Lactobacillus Delbrueckii</i>Strains Isolated from Cow, Sheep and Buffalo Yoghurts

Ivanova, P.; Peykov, Slavil; Dimitrova, Albena; Dimov, Svetoslav

doi:10.1080/13102818.2008.10817545

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…These basic phenotypic methods include morphology, Gram staining and biochemical tests such as fermentation of carbohydrates or growth at varying temperatures and salt concentrations [ 12 ]. Morphology screening for differentiation seems problematic, as it is known that lactobacilli have diverse morphotypes within the same species [ 13 ]. Fortunately, for species-specific identification of strains based on physiological properties other modern tools have become available over the last years such as the API system from Biomérieux (France), the Diatabs system (Rosco, Denmark) or the BIOLOG GP MicroPlate System (BIOLOG Inc., USA).…”

Section: Introductionmentioning

confidence: 99%

Timely approaches to identify probiotic species of the genus Lactobacillus

et al. 2013

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Over the past decades the use of probiotics in food has increased largely due to the manufacturer’s interest in placing “healthy” food on the market based on the consumer’s ambitions to live healthy. Due to this trend, health benefits of products containing probiotic strains such as lactobacilli are promoted and probiotic strains have been established in many different products with their numbers increasing steadily. Probiotics are used as starter cultures in dairy products such as cheese or yoghurts and in addition they are also utilized in non-dairy products such as fermented vegetables, fermented meat and pharmaceuticals, thereby, covering a large variety of products.To assure quality management, several pheno-, physico- and genotyping methods have been established to unambiguously identify probiotic lactobacilli. These methods are often specific enough to identify the probiotic strains at genus and species levels. However, the probiotic ability is often strain dependent and it is impossible to distinguish strains by basic microbiological methods.Therefore, this review aims to critically summarize and evaluate conventional identification methods for the genus Lactobacillus, complemented by techniques that are currently being developed.

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Section: Introductionmentioning

confidence: 99%

Timely approaches to identify probiotic species of the genus Lactobacillus

et al. 2013

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“…Sobotka et al (2004) concluded that AFLP method detected polymorphisms in oilseed rape cultivars more efficiently than either RAPD or SSR methods, but also stated the importance to detect informative markers and confirmed that the results obtained by different marker systems were often similar. The main disadvantage of RAPD method is often reported to be a low reproducibility, but improved laboratory techniques and more exact band scoring help upgrading the poor reproducibility in RAPD analyses (Ivanova et al, 2008). Genetic diversity is best estimated if other markers -agro-morphological, biochemical are also studied (Yu et al, 2005;Galović et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

Paulauskas

Jodinskienė

Griciuvienė

et al. 2013

Zemdirbyste-Agriculture

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Winter oilseed rape is an important crop grown in many countries for its valuable oil and protein. One of the main problems of cultivating rapeseed in northern countries is winter survival. The objective of this study was to assess morphological traits and genetic diversity of eleven cultivars of winter oilseed rape (Brassica napus L.) grown in experimental fields and to determine the correlation between phenotypic variation, deoxyribonucleic acid (DNA) polymorphism and overwintering characteristics. Oilseed rape stand density, height of growth point from the soil surface, thickness of root neck as well as the number of developed leaves per plant were observed for morphological traits analysis. The number of owerwintered plants was evaluated. The genetic variability of 11 oilseed rape cultivars was evaluated by random amplified polymorphic DNA (RAPD) screening 14 primers, 9 of them being polymorphic and used for further analysis. A total of 84 reproducible RAPD bands were identified among 134 individuals, 73 of the detected DNA bands were polymorphic. Among cultivars Nei's gene diversity ranged in the interval 0.1344-0.0313, Shannon's information index was 0.2022-0.0449. An analysis of molecular variance (AMOVA) showed a significant genetic diversity among populations (Phi PT = 0.684, p < 0.01). Unweighted pair group with arithmetic mean (UPGMA) cluster analysis dendrogram generated on the basis of 73 polymorphic RAPD bands showed a clear separation of all analysed cultivars to different clusters. Dendrogram constructed using five morphological traits divided 11 oilseed rape cultivars into two clusters with one stand-alone cultivar 'DK Secure'. No significant correlation of genetic diversity with morphological traits was obtained. A strong and significant correlation between the oilseed rape overwintering capability and the number of polymorphic loci was obtained only in 2008-2009 season (r = 0.81, p = 0.03).

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“…The isolates were first identified on the basis of Gram stain, morphology, catalase reaction and advanced biochemical tests. For molecular identification of the bacteria, genomic DNA of the isolates was extracted using a genomic DNA purification kit (Fermentas), and the isolates were identified by primers specific for the 16S rDNA gene sequences (forward 5 0 -TGG AAA CAG RTG CTA ATA CCG-3 0 and reverse 5 0 -GTC CAT TGT GGA AGA TTC CC-3 0 ; Ivanova et al 2008). The 16S rDNA fragment was amplified using HotStarTaq Master Mix (Qiagen, Ankara, Turkey) in a Techne TC-512 thermocycler (Techne, UK) under the following conditions: 94°C for 2 min, 30 cycles of 94°C for 20 s, 59°C for 30 s, 72°C for 1 min and finally 72°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Determination of the relationship between oxalate degradation and exopolysaccharide production by different Lactobacillus probiotic strains

Sönmez¹,

Darilmaz

Beyatlı³

2018

Int J of Dairy Tech

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The aim of this study was to evaluate the protective effects of exopolysaccharide (EPS) production on resistance to gastrointestinal digestive conditions and oxalate by probiotic strains of the species Lactobacillus rhamnosus, Lactobacillus fermentum and Lactobacillus brevis. The oxalate‐degrading ability of the strains was determined using an enzymatic assay. The correlation between oxalate degradation rate and EPS production was not significant (P > 0.05); however, the high‐EPS‐producing L. fermentumIP5 and L. brevisYG7 strains showed high oxalate‐degrading activity, whereas the low‐EPS‐producing strain L. fermentumBP5 demonstrated low oxalate‐degrading activity. The present findings suggest that dietary supplementation with the probiotic strain L. fermentumIP5 could be a promising strategy for the prevention of oxalate stone disease.

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Molecular Typing by Genus-Specific PCR and Rapd Profiling of DiverseLactobacillus DelbrueckiiStrains Isolated from Cow, Sheep and Buffalo Yoghurts

Cited by 10 publications

References 13 publications

Timely approaches to identify probiotic species of the genus Lactobacillus

Timely approaches to identify probiotic species of the genus Lactobacillus

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

Determination of the relationship between oxalate degradation and exopolysaccharide production by different Lactobacillus probiotic strains

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