Molecular Typing Methods for the Genus Pseudomonas

Libisch, Balázs

doi:10.1007/978-1-62703-185-1_24

Cited by 2 publications

(1 citation statement)

References 62 publications

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“…Besides, PFGE is a relatively inexpensive approach with excellent typeability, high sensitivity ( Abu-Taleb et al, 2013 ), intra-laboratory reproducibility and easy interpretation ( Römling and Tümmler, 2000 ; Ballarini et al, 2012 ). PFGE-SpeI approach is the recommended method for P. aeruginosa genomes study ( Morales et al, 2004 ; Libisch, 2013 ). Macrorestriction patterns generated by restriction enzyme SpeI showed 100% reproducibility and the typeability was in between 95 and 100% ( Grundmann et al, 1995 ).…”

Section: Molecular Typing Methods Of Pseudomonas Aeruginosamentioning

confidence: 99%

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

et al. 2018

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Pseudomonas aeruginosa is a rod-shaped Gram-negative bacterium which is notably known as a pathogen in humans, animals, and plants. Infections caused by P. aeruginosa especially in hospitalized patients are often life-threatening and rapidly increasing worldwide throughout the years. Recently, multidrug-resistant P. aeruginosa has taken a toll on humans’ health due to the inefficiency of antimicrobial agents. Therefore, the rapid and advanced diagnostic techniques to accurately detect this bacterium particularly in clinical samples are indeed necessary to ensure timely and effective treatments and to prevent outbreaks. This review aims to discuss most recent of state-of-the-art molecular diagnostic techniques enabling fast and accurate detection and identification of P. aeruginosa based on well-developed genotyping techniques, e.g., polymerase chain reaction, pulse-field gel electrophoresis, and next generation sequencing. The advantages and limitations of each of the methods are also reviewed.

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Section: Molecular Typing Methods Of Pseudomonas Aeruginosamentioning

confidence: 99%

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

et al. 2018

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Quorum Sensing and Genetic Lineages in Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>

Shafigh,

Pournajaf,

Izadi Amoli

et al. 2024

Jundishapur J Microbiol

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Background: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has become a major global concern. Quorum sensing (QS) regulates the expression of biofilm formation genes and virulence factors. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) is widely used in epidemiological molecular studies. Objectives: The purpose of the present study was to determine the QS characteristics and genetic relatedness of CRPA. Methods: A total of 57 non-duplicative CRPA isolates were collected. A microtiter plate assay was used to assess biofilm formation. After DNA extraction, PCR was performed to detect resistance elements and QS-encoded genes. Enterobacterial repetitive intergenic ERIC-PCR was conducted using specific primers. Results: The biofilm formation assay revealed that 10.5%, 19.3%, and 70.2% of isolates formed weak, moderate, and strong biofilms, respectively. Of the isolates, 75.4%, 64.9%, 12.3%, and 8.7% carried the blaIMP, blaVIM, blaNDM, and blaKPC genes, respectively. Additionally, 73.7%, 7.0%, and 1.7% of CRPA isolates carried the blaOXA-48-like, blaOXA-23-like, and blaOXA-20/40-like genes, respectively. The prevalence of the lasR, lasI, rhlI, rhlR, aprR, aprA, and rhlAB genes were 100%, 96.5%, 92.9%, 89.5%, 84.2%, 73.6%, and 63.2%, respectively. Enterobacterial repetitive intergenic ERIC-PCR revealed eight distinct clusters (A, B, C, D, E, F, G, and H) using a similarity cut-off of ≥ 60%. Conclusions: The findings indicate a high prevalence of strong biofilm formation and quorum-sensing genes among CRPA isolates. The study highlights the importance of biofilm production and genetic diversity in CRPA isolates, underscoring the challenges in infection control and treatment strategies.

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Molecular Typing Methods for the Genus Pseudomonas

Cited by 2 publications

References 62 publications

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

Quorum Sensing and Genetic Lineages in Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>

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