Molecularly defined circuits for cardiovascular and cardiopulmonary control

Abstract

The sympathetic and parasympathetic nervous systems powerfully regulate internal organs 1 , but the molecular and functional diversity of their constituent neurons and circuits remains largely unknown. Here we use retrograde neuronal tracing, single-cell RNA sequencing, optogenetics, and physiological experiments to dissect the cardiac parasympathetic control circuit in mice. We show that cardiac-innervating neurons in the brainstem nucleus ambiguus (Amb) are comprised of two molecularly, anatomically, and functionally distinct subtypes. One we call ACV (ambiguus cardiovascular) neurons (~35 neurons per Amb), define the classical cardiac parasympathetic circuit. They selectively innervate a subset of cardiac parasympathetic ganglion neurons and mediate the baroreceptor reflex, slowing heart rate and atrioventricular node conduction in response to increased blood pressure. The other, ACP (ambiguus cardiopulmonary) neurons (~15 neurons per Amb) innervate cardiac ganglion neurons intermingled with and functionally indistinguishable from those innervated by ACV neurons, but surprisingly also innervate most or all lung parasympathetic ganglion neurons; clonal labeling shows individual ACP neurons innervate both organs. ACP neurons mediate the dive reflex, the simultaneous bradycardia and bronchoconstriction that follows water immersion. Thus, parasympathetic control of the heart is organized into two parallel circuits, one that selectively controls cardiac function (ACV circuit) and another that coordinates cardiac and pulmonary function (ACP circuit). This new understanding of cardiac control has implications for treating cardiac and pulmonary diseases and for elucidating the control and coordination circuits of other organs.

BrainstemDbh+Neurons Control Chronic Allergen-Induced Airway Hyperreactivity

“…6c-h). Our neuron number and neuron type clusters are comparable to the two recent published NA single cell datasets 45,46 .…”

Microglia-mediated inflammatory destruction of neuro-cardiovascular dysfunction after stroke

“…Amb is composed of two molecules that are anatomically and functionally distinct subtypes called ambiguus cardiopulmonary (ACP) and ambiguus cardiovascular (ACV). ACV will sense changes in the peripheral blood pressure and selectively activate the cholinergic cardiovascular ganglion neurons, which regulate the cardiovascular function, whereas ACP is recently found and activates the dive reflex which can also induce bronchoconstriction simultaneously (Veerakumar et al, 2022 ). Ching-Yi Tsai used DTI to revise the baroreflex neural circuits that consist of nucleus tractus solitarii (NTS) and Amb or the rostral ventrolateral medulla (RVLM) and the caudal ventrolateral medulla (CVLM).…”

Ageing impairs the neuro-vascular interface in the heart

“…Next, we assessed which types of nerves are affected by aging. The heart is innervated by sympathetic, parasympathetic and sensory fibers, which are commonly stained for tyrosine hydroxylase (TH) 16,17 , choline acetyltransferase (ChAT) 18,19 and calcitonin gene-related peptide (CGRP) 20 , respectively. TH-positive nerves were present in both ventricles, but were selectively reduced in the left ventricle of aged mice ( Fig.1f ).…”