Molecularly imprinted and cladded nanoparticles for high-affinity recognition of structurally closed gangliosides

Synthesis of molecularly imprinted polymer based on cooperative imprinting for enrichment of gallic acid in Puer tea

“…39 The absorption peaks of AAPBA MIP and AAPBA NIP at 3700-3200 cm À1 may be assign to the stretching vibrations of borate groups or the hydrogen bond that AAPBA self-polymerize formed. 37,38 all FTIR spectra of MIPs presented a characteristic absorption peak at around 1636 cm À1 may be assigned to the residual C C stretching vibration group of AAPBA and MAA. It was observed that the intensity of the peak increased than that of NIPs, indicating that most of the monomer was polymerized in MIPs.…”

“…As shown in Figure 2, the MAA MIP displayed the worst bond capacity, which may be caused by the embedding of imprinted cavities under the conventional imprinted strategy. 36 Similarly, the AAPBA MIP exhibited the lowest imprinting factor (1.078), although the adsorption capacity (0.067 ± 0.001 mmol g À1 ) was higher than that of MAA MIP, the probable explanation may be that the AAPBA also acted as self-polymerizing monomer, which leaded to the less specific imprinted sites and displayed the nonspecific adsorption for GA. 37,38 Yet, we turned our attention to the incorporation of MAA and AAPBA in the system to form a ternary polymer of poly (MAA-EDMA-AAPBA), it could be observed the AAPBAco-MAA MIP demonstrated stronger imprinting effect (Q e = 0.217 ± 0.018 mmol g À1 , IF = 2.21) than any other single-monomer MIPs, which was primarily attributed to the double affinity of covalent and non-covalent recognition interactions enhanced the selectivity of polymer. In comparison with the other MIPs based on different monomers reported in the previous literature, as shown in Table 2, the adsorption capacity of GA on AAPBA-co-MAA MIP prepared in this work was improved significantly.…”

Glycan-specific molecularly imprinted polymers towards cancer diagnostics: merits, applications, and future perspectives

“…Despite extensive research on the preparation of glycanspecific MIPs, [30][31][32][33][34] their incorporation into liquid biopsybased diagnostics has not been widely explored. This could be attributed to the challenges involved in obtaining pure glycan templates that are specifically associated with diseases and the incompatibility of traditional MIPs, which are often hydrophobic and unsuitable for aqueous-based biological applications.…”

“…Compared to natural receptors, MIPs offer significant advantages primarily due to their broad generality. Reported studies have demonstrated the capability of MIPs to bind a wide range of glycans, encompassing N-glycans, 30 O-glycans, 31 glycosaminoglycans, 32,33 gangliosides 34 and so on. Importantly, MIPs exhibit selectivity and affinity towards these targets that can be comparable to antibodies and lectins.…”

“…Molecularly imprinted polymers (MIPs), a category of artificial receptors with custom-designed binding sites for targeted molecules, 29 present a viable solution to address these challenges. Specifically, customized MIPs possess the ability to selectively bind to a wide range of anomalous glycans, including N-glycans, 30 O-glycans, 31 glycosaminoglycans, 32,33 and gangliosides, 34 with remarkable affinity. This capacity holds the potential to enable clinical translation of numerous MSidentified glycans that lack specific antibodies, effectively broadening the scope and applicability of glycan-based diagnostics.…”