Molecularly imprinted “bulk” copolymers as selective sorbents for gallic acid

Nicolescu, Tanta-Verona; Sârbu, Andrei; Dima, Ștefan-Ovidiu; Nicolae, Cristian‐Andi; Donescu, Dan

doi:10.1002/app.37528

Cited by 30 publications

(28 citation statements)

References 35 publications

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“…The AIFI of MIPs in the presence of BSA or BGG was similar to all NIP samples with overlapping SD values; thus, there was no statistical difference between any of the controls. These factors compare favourably with literature where imprinting factors of 4-8 and selectivity coefficients of 5-7 have been reported [29][30][31]. All p-values were below 0.0015 when comparing MIPs to NIPs or any of its controls.…”

Section: Mip Binding Characteristicssupporting

confidence: 84%

Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays

Rincon

Santillan

Infante

et al. 2017

J Biotechnol Biomater

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A new molecular imprinting technique was developed for molecularly imprinted polymer particles (MIPs). Particles were synthesized using organic silane chemistries by a sol-gel process, where the relative amount of active monomers was complementary matched to the relative amount of surface charges of the West Nile antibody template. Synthesized MIPs showed specific binding to affinity purified polyclonal West Nile antibodies (WNA) with a loading capacity of 80 µg/mg, while MIPs absorbed non-specific proteins at a loading capacity of 28 µg/mg. A dissociation constant of Kd=57.45 μM was measured from the binding isotherms. MIPs selectively absorbed 27 times more WNA than either albumin or immunoglobulin, while MIPs absorbed 16 times more WNA than nonimprinted particles (NIPs). Finally, fluorescently labeled MIPs were incubated in a high bind 96 well plate previously loaded with template, albumin, or immunoglobulin as an immunoassay test. Fluorescent MIPs significantly bound more to wells with WNA than any other control. Thus, the development of new affordable and robust immunoassays with MIPs would be possible in the future.

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Section: Mip Binding Characteristicssupporting

confidence: 84%

Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays

Rincon

Santillan

Infante

et al. 2017

J Biotechnol Biomater

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“…Scientists have proved that GA has in vitro and in vivo anticancer efficacy against breast, prostate, and lung cells without harming healthy cells . GA is an important precursor for many synthetic compounds and also usually used in manufacturing antioxidants, ink dyes, fuels, and paper‐making . Owing to its pharmaceutical and industrial significance, the demand for GA has been increased significantly.…”

Section: Introductionmentioning

confidence: 99%

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Zhang

Yue

et al. 2017

J of Separation Science

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With the combined surface imprinting technique and immobilized template strategy, molecularly imprinted magnetic nanoparticles were successfully prepared and coupled with high-performance liquid chromatography to selectively separate and determine gallic acid from the pomegranate rind. On the surface of carboxyl-functionalized magnetic nanospheres, thin imprinting shells were formed using dopamine as monomer and crosslinker. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of good crystallinity, satisfactory magnetism, and uniform morphology of the obtained polymers, they had rapid binding kinetics, high adsorption capacity, and favorable reusability. In the mixed solution of four hydroxybenzoic acids, the prepared nanomaterials have an excellent selectivity to gallic acid with an imprinting factor of as high as 17.5. Therefore, the polymers have great potentials in specific extraction and enrichment of gallic acid from the complex natural resources.

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“…This step appears in the DTG diagram as a shoulder or a small semi-convoluted peak. The second thermal degradation step, situated in the interval 375-650°C, is common to all systems and represents the acrylonitrile aromatization and graphitization [9][10][11][12][13][14]. The imprinted polymer is the most thermally sensitive, meaning that sclareol, similar to diosgenin, is the one which is firstly decomposing (with a maximum at 112.7°C).…”

Section: Tg and Dtg Analyses Of Mips And Nipsmentioning

confidence: 99%

“…Among these types of analyses, an important role is represented by thermal analysis. The thermal methods most often used for MIP characterization are the thermogravimetric analysis (TG), sometimes coupled with mass spectrometry (MS) or Fourier transform infrared spectroscopy (FT-IR), the differential scanning calorimetry (DSC), pyrolysis, and dynamic mechanical thermal analysis (DMTA) [7][8][9][10][11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Thermal analyses as tools for proving the molecular imprinting with diosgenin and sclareol in acrylic copolymer matrices

Dima

Nicolae

Iordache

et al. 2015

J Therm Anal Calorim

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Thermogravimetric analysis (TG), derivative thermogravimetry (DTG), differential scanning calorimetry (DSC), dynamic mechanical thermal analysis, simultaneous differential thermogravimetry (SDT) coupled with mass spectrometry (MS), and pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC/MS) were used to characterize molecularly imprinted polymers (MIPs) obtained with two anti-cancerous template molecules, diosgenin and sclareol, imprinted in acrylonitrileacrylic acid copolymer matrices. First of all, TG analyses revealed a specific mass loss in the temperature range 220-300°C characteristic to diosgenin-MIPs, while for the sclareol-MIPs, the specific interval was 100-240°C. Further, the DSC analyses evidenced two major aspects: first, that the copolymer matrices have a single glass transition temperature (T g ), which confirms the random statistic copolymer structure, an essential characteristic of a homogenous MIP matrix; secondly, both templates influence the T g values of the MIPs, fact that is an additional proof of molecular imprinting. The SDT results confirmed the TG and DSC results and, additionally, proved that the system with higher acrylic acid content generates stronger template-matrix interactions via hydrogen, van der Waals, and other non-covalent forces, fact that appears as an increased thermal stability on the TG curve and a higher maximum for template decomposition on the DTG curve. The MS results evidenced that a single mass fragment, 139 ± 1 m/z, characteristic for the class of steroid sapogenines of which diosgenin belongs, is enough to prove, qualitatively and quantitatively, the molecular imprinting and the template elution. As global conclusion, the applied thermal analyses proved to be exceptional tools for analyzing these molecularly imprinted polymers.

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Molecularly imprinted “bulk” copolymers as selective sorbents for gallic acid

Cited by 30 publications

References 35 publications

Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays

Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Thermal analyses as tools for proving the molecular imprinting with diosgenin and sclareol in acrylic copolymer matrices

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