Molecularly Imprinted Electroimpedance Sensor for Detection of 8-isoprostane in Exhaled Breath Condensate

Abstract

Development of low-cost, rapid response time capacitive sensors have a valuable role in the creation of point-of-care systems. A novel approach in the materials and application of Molecularly Imprinted Polymers was investigated for the detection of 8isoprostane in exhaled breath condensate. The detection method is based on the quantifiable capacitance change that occurs between two electrodes as the target molecule binds on the MIPs surface, which is detected through electrochemical impedance spectroscopy. This work focuses on the use of a generic polymer material for the sensing layer, as opposed to a traditional synthesized polymer material. PVA-SbQ was spun onto a custom IDE and then imprinted to detect 8-isoprostance. With aerosolized samples, the sensor was proven to detect a physiologically relevant concentration of 1 to 100 pg/mL. A fully integrated multiplexed system was then developed for point-of-care health monitoring.