Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein

Bartold, Katarzyna; Iskierko, Zofia; Borowicz, Paweł; Noworyta, Krzysztof; Lin, Chu-Yun; Kalecki, Jakub; Sharma, Piyush Sindhu; Lin, Hung-Yin; Kutner, Włodzimierz

doi:10.1016/j.bios.2022.114203

Cited by 34 publications

(25 citation statements)

References 29 publications

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“…Molecularly imprinted polymers (MIPs) have been synthesized for the recognition of target molecules, and have been widely used in bioseparations [ 8 , 9 , 10 ], biosensing [ 8 , 11 , 12 , 13 , 14 ], and medicines [ 15 , 16 ]. Zhang’s group developed epitope-imprinted nanoparticles for the recognition of oxytocin [ 17 ], bovine serum albumin [ 18 , 19 ], tyrosine phosphopeptide [ 20 ], human serum albumin [ 21 ], and cytochrome c [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Lee

Jan

Thomas

et al. 2022

Cells

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Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.

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Section: Introductionmentioning

confidence: 99%

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Lee

Jan

Thomas

et al. 2022

Cells

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“…That decreased the differential pulse voltammetry (DPV) peak current, resulting in a sensor signal that varied linearly with the protein concentration of 1 to 200 ng/mL, with a limit of detection of 0.3 ng/mL [ 23 ]. Our previous work demonstrated the optimized molar ratio of functional monomers to templates with the linear dynamic concentration range of 50–500 nM [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Peptide Selection of MMP-1 for Electrochemical Sensing with Epitope-Imprinted Poly(TPARA-co-EDOT)s

et al. 2022

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Instead of molecularly imprinting a whole protein molecule, imprinting protein epitopes is gaining popularity due to cost and solubility issues. Belonging to the matrix metalloproteinase protein family, MMP-1 is an interstitial collagenase that degrades collagen and may be involved in cell migration, cell proliferation, the pro-inflammatory effect, and cancer progression. Hence, it can serve as a disease protein biomarker and thus be useful in early diagnosis. Herein, epitopes of MMP-1 were identified by screening its crystal structure. To identify possible epitopes for imprinting, MMP-1 was cleaved in silico with trypsin, pepsin at pH = 1.3, and pepsin at pH > 2.0 using Peptide Cutter, generating peptide fragments containing 8 to 12 amino acids. Five criteria were applied to select the peptides most suitable as potential epitopes for MMP-1. The triphenylamine rhodanine-3-acetic acid (TPARA) functional monomer was synthesized to form a stable pre-polymerization complex with a selected template epitope. The complexed functional monomer was then copolymerized with 3,4-ethoxylenedioxythiophene (EDOT) using potentiodynamic electropolymerization onto indium–tin–oxide (ITO) electrodes. The composition of the molecularly imprinted poly(TPARA-co-EDOT) (MIP) was optimized by maximizing the film’s electrical conductivity. Cyclic voltammetry was used to determine MMP-1 concentration in the presence of the Fe(CN)63−/Fe(CN)64− redox probe actuating the “gate effect.” A calibration curve was constructed and used to determine the usable concentration range and the limit of detection as ca. 0.001 to 10.0 pg/mL and 0.2 fg/mL MMP-1, respectively. Finally, the MMP-1 concentration in the A549 human lung (carcinoma) culture medium was measured, and this determination accuracy was confirmed using an ELISA assay.

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“…Such a standard MOSFET can also be used in liquid-sample FET-based (bio)sensing in an extended-gate configuration (EGFET) [8][9][10] (see the scheme in Fig. 1B).…”

Section: Introductionmentioning

confidence: 99%

“…Here, a MOSFET acts as a transducer with the sensing surface attached to its gate (typically either a glass pH electrode used herein or e.g. a gold electrode with thiol chemistry coupled specific receptors [8][9][10] ) forming an extended gate, which is submerged in the liquid solution along with a reference electrode (e.g. Ag/AgCl).…”

Section: Introductionmentioning

confidence: 99%

Facilitating field-effect transistor based (bio)sensor research with a miniaturized bipotentiostat

Filipiak

Wróblewska

Suranglikar

et al. 2022

Preprint

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Field-effect transistors (FETs) have been around for more than half a century and are at the heart of everyday electronics, but field-effect transistor based (bio)sensors are an emerging field with a huge potential that is yet to be explored. These (bio)sensors are useful in the detection of various analytes including ions, small organic molecules, DNA, proteins, and cells. However, the equipment such as parameter analysers or source meters used to characterize FET-based (bio)sensors is typi-cally bulky, expensive and complex, limiting the access to their research and development. There is a need for a compact and portable solution to this problem. It is worth noting that an answer to this conundrum may lie in the knowledge gap between the research communities of electrical/material engineers or physicists working on FETs and the electrochemists focused on potentiometry, which herein we aim to bridge. We present an economical and versatile solution based on an original equipment manufacturer (OEM) product from a leading portable potentiostat developer – PalmSens. Together with their engineers, we repurpose the flexible and affordable EmStat Pico bipotentiostat module to measure a variety of differ-ent commercially available FET sensor architectures – starting from a simple metal-oxide-semiconductor FET (MOSFET) which is then used as a transducer in extended-gate configuration. We then move towards a more complex ion-sensitive FET and a state-of-the-art single-sheet graphene electrolyte gated FET. Using a simple proprietary scripting language, we perform proof-of-concept experiments aimed at popularizing the FET field among electrochemists. We believe that using the EmStat Pico module can help accelerate FET-based (bio)sensor research and bridge the knowledge gap between FET researchers: physicists, electrical/material engineers and chemists. On top of that, the mentioned bipotentiostat is ready to implement in an end-user device, accelerating the commercialization of FET (bio)sensors for e.g. medical diagnostics.

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Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein

Cited by 34 publications

References 29 publications

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Peptide Selection of MMP-1 for Electrochemical Sensing with Epitope-Imprinted Poly(TPARA-co-EDOT)s

Facilitating field-effect transistor based (bio)sensor research with a miniaturized bipotentiostat

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