Molecularly imprinted polymer thin film based surface plasmon resonance sensor to detect hemoglobin

Wang, Yang; Zhang, Qingwen; Ren, Yamin; Jing, Lijing; Wei, Tianxin

doi:10.1007/s40242-013-3330-1

Cited by 26 publications

(15 citation statements)

References 47 publications

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“…The application of pulsed potential aids also the replenishment of the depleted solution layer adjacent to the electrode by the low diffusivity macromolecular template [79], [83], and thus to enhance the incorporation of the protein template into the growing polymer.…”

Section: Functional Monomers For the Electrosynthesis Of Protein Mipsmentioning

confidence: 99%

“…This opportunity, although well exploited in case of chemically synthesized pAPBA MIPs [75], [76], has not been employed with electropolymerized films, which have only been prepared to date for the recognition of BSA [77], lysozyme and cytochrome c [78]; all non-glycated proteins. Wang et al imprinted pAPBA with hemoglobin, a protein that exists to a minor extent in glycated form as well, but there is no mention on the exact form of the protein [79]. Future works with electrosynthesized pAPBA MIPs for glycoprotein recognition should consider prior immobilization of the template glycoprotein, which was found essential in obtaining satisfactory selectivity against other glycoproteins [76].…”

Section: Functional Monomers For the Electrosynthesis Of Protein Mipsmentioning

confidence: 99%

“…Wang et al incubated the electrode in the monomer-template mixture to allow for protein adsorption on the surface before starting the polymerization [79] (Fig. 3B).…”

Section: Strategies For the Electrosynthesis Of Protein-imprinted Polmentioning

confidence: 99%

“…The essential prerequisite for any procedure is not to alter the polymer structure in an extent that would affect the rebinding of the template. This is not as straightforward as many studies suggest and in fact the choice of the regeneration solvent is a trade-off between complete removal of the imprinted protein [56], [71] and preservation of the integrity of the binding sites [79], [87]. Accordingly, different methods were applied that range from the application of chaotropic agents to the denaturation, dissociation or lysis of the protein.…”

Section: Template Removalmentioning

confidence: 99%

“…This method is applicable to imprints created with free or physically immobilized proteins. A wide range of treatments were reported including the use of highly acidic [6], [84], [85] or basic [55] solutions and/or surfactants (sodium dodecylsulfate (SDS) [68], [89] or Tween 20 [78], [79]), sometimes at elevated temperatures [62], [6], [70]. For oligomeric proteins the dissociation into monomeric units by changing the pH and/or employing detergents may also be used to generate free binding sites in the MIP.…”

Section: Template Removalmentioning

confidence: 99%

See 4 more Smart Citations

Electrosynthesized molecularly imprinted polymers for protein recognition

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TrAC Trends in Analytical Chemistry

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Section: Functional Monomers For the Electrosynthesis Of Protein Mipsmentioning

confidence: 99%

Section: Functional Monomers For the Electrosynthesis Of Protein Mipsmentioning

confidence: 99%

“…Wang et al incubated the electrode in the monomer-template mixture to allow for protein adsorption on the surface before starting the polymerization [79] (Fig. 3B).…”

Section: Strategies For the Electrosynthesis Of Protein-imprinted Polmentioning

confidence: 99%

Section: Template Removalmentioning

confidence: 99%

Section: Template Removalmentioning

confidence: 99%

See 3 more Smart Citations

Electrosynthesized molecularly imprinted polymers for protein recognition

Erdőssy

Horváth²,

Yarman

et al. 2016

TrAC Trends in Analytical Chemistry

155

133

View full text Add to dashboard Cite

Molecular Imprinting Technique for Biomimetic Sensing and Diagnostics

Zhang

Zhao

Yang

2016

Biomedical Nanomaterials

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Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

et al. 2015

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Hybrid architectures which combine a MIP with an immobilized “affinity ligand” or a biocatalyst sum up the advantages of both components. In this paper, hybrid architectures combining a layer of a molecularly imprinted electropolymer with a mini‐enzyme or a self‐assembled monolayer will be presented. (i) Microperoxidase‐11 (MP‐11) catalyzed oxidation of the drug aminopyrine on a product‐imprinted sublayer: The peroxide dependent conversion of the analyte aminopyrine takes place in the MP‐11 containing layer on top of a product‐imprinted electropolymer on the indicator electrode. The hierarchical architecture resulted in the elimination of interfering signals for ascorbic acid and uric acid. An advantage of the new hierarchical structure is the separation of MIP formation by electropolymerization and immobilization of the catalyst. In this way it was for the first time possible to integrate an enzyme with a MIP layer in a sensor configuration. This combination has the potential to be transferred to other enzymes, e.g. P450, opening the way to clinically important analytes. (ii) Epitope‐imprinted poly‐scopoletin layer for binding of the C‐terminal peptide and cytochrome c (Cyt c): The MIP binds both the target peptide and the parent protein almost eight times stronger than the non‐imprinted polymer with affinities in the lower micromolar range. Exchange of only one amino acid in the peptide decreases the binding by a factor of five. (iii) MUA‐poly‐scopoletin MIP for cytochrome c: Cyt c bound to the MIP covered gold electrode exhibits direct electron transfer with a redox potential and rate constant typical for the native protein. The MIP cover layer suppresses the displacement of the target protein by BSA or myoglobin. The combination of protein imprinted polymers with an efficient electron transfer is a new concept for characterizing electroactive proteins such as Cyt c. The competition with other proteins shows that the MIP binds its target Cyt c preferentially and that molecular shape and the charge of protein determine the binding of interfering proteins.

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Molecularly imprinted polymer thin film based surface plasmon resonance sensor to detect hemoglobin

Cited by 26 publications

References 47 publications

Electrosynthesized molecularly imprinted polymers for protein recognition

Electrosynthesized molecularly imprinted polymers for protein recognition

Molecular Imprinting Technique for Biomimetic Sensing and Diagnostics

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

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