Molecularly imprinted polymer with mixed-mode mechanism for selective extraction and on-line detection of ochratoxin A in beer sample

Liu, Yue; Su, Zhiping; Wang, Jun; Gong, Zhaoning; Lyu, Haixia; Xie, Zenghong

doi:10.1016/j.microc.2021.106696

Cited by 11 publications

(5 citation statements)

References 42 publications

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“…In all these cases (except [ 105 ], where ochratoxin A is directly quantified without any further separative step), as the extraction step is followed by separation on an HPLC analytical column, the critical step for a successful method consists of the use of a desorption eluent compatible with the analytical eluent used in the HPLC column. This step therefore involves methanol/triethylamine as the desorption element and methanol/ammonium buffer as the analytical eluent [ 100 , 101 , 102 ], methanol/acetic acid as both the desorption and analytical eluent [ 103 ], methanol/acetic acid as the desorption element, and acetonitrile/water/acetic acid as the analytical eluent [ 104 , 105 , 106 ]. The on-line extraction of beer [ 103 ], wheat extracts [ 105 , 106 ], and red wine [ 100 , 101 , 102 ] resulted in being selective for the target analyte and allowed for the quantification of the contamination levels in these matrices being well below the legal limits established by the health authorities.…”

Section: Use Of Mips In Sample Preparation For Mycotoxin Detectionmentioning

confidence: 99%

“…This step therefore involves methanol/triethylamine as the desorption element and methanol/ammonium buffer as the analytical eluent [ 100 , 101 , 102 ], methanol/acetic acid as both the desorption and analytical eluent [ 103 ], methanol/acetic acid as the desorption element, and acetonitrile/water/acetic acid as the analytical eluent [ 104 , 105 , 106 ]. The on-line extraction of beer [ 103 ], wheat extracts [ 105 , 106 ], and red wine [ 100 , 101 , 102 ] resulted in being selective for the target analyte and allowed for the quantification of the contamination levels in these matrices being well below the legal limits established by the health authorities. Aside from ochratoxin A, on-line SPE in a packed microcolumn has been reported for citrinin in red yeast rice extracts [ 107 ] and patulin in apple juice [ 108 , 109 ].…”

Section: Use Of Mips In Sample Preparation For Mycotoxin Detectionmentioning

confidence: 99%

“…In this case, the typology of the imprinted stationary phases appears to be quite varied and includes stainless steel frits grafted with a thin layer of electropolymerized polypyrrole [100][101][102] and an imprinted monolith prepared in a bare fused-sil-Ochratoxin A is a target analyte towards which numerous examples of on-line SPE have been reported. In this case, the typology of the imprinted stationary phases appears to be quite varied and includes stainless steel frits grafted with a thin of electropolymerized polypyrrole [100][101][102] and an imprinted monolith prepared in a bare fused-silica capillary [103] or a HPLC microcolumn [104], but also traditional HPLC precolumns packed with an MIP prepared via bulk polymerization [104,105]. In all these cases (except [105], where ochratoxin A is directly quantified without any further separative step), as the extraction step is followed by separation on an HPLC analytical column, the critical step for a successful method consists of the use of a desorption eluent compatible with the analytical eluent used in the HPLC column.…”

Section: On-line Solid-phase Extractionmentioning

confidence: 99%

See 2 more Smart Citations

Mycotoxins-Imprinted Polymers: A State-of-the-Art Review

Cavalera,

Anfossi,

Di Nardo

et al. 2024

Toxins

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Mycotoxins are toxic metabolites of molds which can contaminate food and beverages. Because of their acute and chronic toxicity, they can have harmful effects when ingested or inhaled, posing severe risks to human health. Contemporary analytical methods have the sensitivity required for contamination detection and quantification, but the direct application of these methods on real samples is not straightforward because of matrix complexity, and clean-up and preconcentration steps are needed, more and more requiring the application of highly selective solid-phase extraction materials. Molecularly imprinted polymers (MIPs) are artificial receptors mimicking the natural antibodies that are increasingly being used as a solid phase in extraction methods where selectivity towards target analytes is mandatory. In this review, the state-of-the-art about molecularly imprinted polymers as solid-phase extraction materials in mycotoxin contamination analysis will be discussed, with particular attention paid to the use of mimic molecules in the synthesis of mycotoxin-imprinted materials, to the application of these materials to food real samples, and to the development of advanced extraction methods involving molecular imprinting technology.

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Section: Use Of Mips In Sample Preparation For Mycotoxin Detectionmentioning

confidence: 99%

Section: Use Of Mips In Sample Preparation For Mycotoxin Detectionmentioning

confidence: 99%

Section: On-line Solid-phase Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Mycotoxins-Imprinted Polymers: A State-of-the-Art Review

Cavalera,

Anfossi,

Di Nardo

et al. 2024

Toxins

View full text Add to dashboard Cite

show abstract

“…The A MIP in a capillary with an internal diameter of 100 μm was also used for extraction and online detection of ochratoxin A in the beer sample. 117 The MIPs were prepared by the radical polymerization reaction of acrylamide-2-methylpropanesulfonic acid, methacrylate substituted polyhedral oligomeric silsesquioxanes, ethylene glycol dimethacrylate and ochratoxin A as a template molecule. The recovery rate of MIP for ochratoxin A was determined to be 84.8%, while nonimprinted polymer provided only 4.5%.…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

Current trends in the development of polymer‐based monolithic stationary phases

Nechvátalová

Urban

2022

Analytical Science Advances

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This review focuses on the development and applications of organic polymer monoliths, with special attention to the literature published in 2021. The latest protocols in the preparation of polymer monoliths are discussed. In particular, tailored surface modification using nanomaterials, the development of chiral stationary phases and development of stationary phases for capillary electrochromatography are reviewed.Furthermore, the optimization of pore forming solvents composition is also discussed.Finally, the use of monolithic stationary phases in sample treatment using solid-phase extraction and enrichment methods, molecularly imprinted polymers and enzymatic reactors is mentioned.

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“…Various types of solid-phase extraction and liquid-liquid extraction methods have been used for sample preparation in food control laboratories and routine practice (Visconti et al 2001;Pena et al 2005;Aresta et al 2006). The analytical parameters of other methods for OTA analysis are summarized in Table 1 (Tessini et al 2010;Liu et al 2021;Gupta et al 2017;Lv et al 2017;Lv et al 2014;Karczmarczyk et al 2016;Anfossi et al 2013;Khan and Dhayal 2008;Dai et al 2016;Taradolsirithitikul et al 2017;Zou et al 2016). In the present study, a one-step immunoassay based on switching peptides was applied for effective OTA analysis in wines with a far shorter analysis time than the HPLC method.…”

Section: Introductionmentioning

confidence: 99%

One-step immunoassay based on switching peptides for analyzing ochratoxin A in wines

et al. 2022

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A one-step immunoassay is presented for the detection of ochratoxin A (OTA) using an antibody complex with switching peptides. Because the switching peptides (fluorescence-labeled) were able to bind the frame region of antibodies (IgGs), they were dissociated from antibodies immediately when target analytes were bound to the binding pockets of antibodies. From the fluorescence signal measurements of switching peptides, a quantitative analysis of target analytes, via a one-step immunoassay without any washing steps, could be performed. As the first step, the binding constant (KD) of OTA to the antibodies was estimated under the continuous flow conditions of a surface plasmon resonance biosensor. Then, the optimal switching peptide, among four types of switching peptides, and the reaction condition for complex formation with the switching peptide were determined for the one-step immunoassay for OTA analysis. Additionally, the selectivity test of one-step immunoassay for OTA was carried out in comparison with phenylalanine and zearalenone. For the application to the one-step immunoassay to detect OTA in wines, two types of sample pre-treatment methods were compared: (1) a liquid extraction was carried out using chloroform as a solvent with subsequent resuspension in phosphate-buffered saline (total analysis time < 1 h); (2) direct dilution of the wine sample (total analysis time < 0.5 h). Finally, the direct dilution method was found to be effective for the one-step immunoassay based on the switching peptide assay for OTA in wines with a markedly improved total analysis time (< 0.5 h). Additionally, the assay results were compared with commercial lateral flow immunoassay.

show abstract

Molecularly imprinted polymer with mixed-mode mechanism for selective extraction and on-line detection of ochratoxin A in beer sample

Cited by 11 publications

References 42 publications

Mycotoxins-Imprinted Polymers: A State-of-the-Art Review

Mycotoxins-Imprinted Polymers: A State-of-the-Art Review

Current trends in the development of polymer‐based monolithic stationary phases

One-step immunoassay based on switching peptides for analyzing ochratoxin A in wines

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